We narrowed to 34,848 results for: CaS;

1. pCAG-T3-SpCAS9-NG-pA

   Plasmid

   #131465

   Purpose

   Expresses SpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroys

   Depositor

   Wataru Fujii

   Article

   Fujii et al Sci Rep. 2019 Sep 9;9(1):12878. doi: 10.

   Insert

   Codon optimized SpCas9-NG
   

   Use

   CRISPR

   Tags

   Flag, NLS, and polyA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. dSaCas9-KRAB

   Plasmid

   #205250

   Purpose

   In vitro transcription plasmid for production of dSaCas9-KOX1 KRAB mRNA for transcriptional activation (KRAB = Krüppel-associated box). Insert is derived from Addgene plasmid #106219.

   Depositor

   Rasmus Bak

   Insert

   dSaCas9-KRAB
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRhaQCas-BsaI

   Plasmid

   #170631

   Purpose

   Expression of CRISPR-associated transposases

   Depositor

   Sheng Yang

   Article

   Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

   Insert

   VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(BsaI)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pXFokI-dCas9

   Plasmid

   #60901

   Purpose

   Dual Expression Vector for FokI-dCas9 and gRNA

   Depositor

   Bruce Conklin

   Article

   Miyaoka et al Sci Rep. 2016 Mar 31;6:23549. doi: 10.10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available Since

   March 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. 2X_pX458_pSpCas9(BB)-2A-GFP_SOX17_bKO

   Plasmid

   #172225

   Purpose

   Cas9-2A-GFP expression vector bearing two sgRNAs targeting the centromeric human SOX17 CTCF-boundary.

   Depositor

   Alexander Meissner

   Article

   Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

   Insert

   sgRNA
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available Since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX165-evoCas9

   Plasmid

   #140569

   Purpose

   Expresses evoCas9 in mammalian cells

   Depositor

   Feng Zhang

   Article

   Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Mutation

   M495V Y515N K526E R661Q

   Promoter

   Available Since

   May 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. UAST-LbCas12a

   Plasmid

   #140621

   Purpose

   Gal4 dependent expression of LbCas12a in Drosophila

   Depositor

   Michael Boutros

   Article

   Port et al Proc Natl Acad Sci U S A. 2020 Aug 25. p

   Insert

   LbCas12a
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   UAST

   Available Since

   Nov. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLBH546_Tet-Cas14b1Locus

   Plasmid

   #112503

   Purpose

   Expresses Cas14b1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

   Depositor

   Jennifer Doudna

   Article

   Harrington et al Science. 2018 Oct 18. pii: science.aav42

   Insert

   Cas14b1
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Tet

   Available Since

   Oct. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV_Anc689-nCas9

   Plasmid

   #163526

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   Anc689-nCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)

   Promoter

   Available Since

   Dec. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYPQ-NF-L1-dMb2Cas12a

    Plasmid

    #177683

    Purpose

    Gateway entry clone (attL1 & attR5) for dimeric FokI-dMb2Cas12a genome editing

    Depositor

    Yiping Qi

    Article

    Cheng et al Biotechnol J. 2022 Apr 4:e2100571. doi:

    Insert

    FokI-dMb2Cas12a
    

    Use

    CRISPR; Gateway compatible foki-dmb2cas12a entry …

    Tags

    NLS

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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