We narrowed to 24,458 results for: nes

1. UbC-Halo-SFPQY527A

   Plasmid

   #166947

   Purpose

   Lentiviral plasmid expressing Halo-tagged SFPQ Y527A protein from the UbC promoter

   Depositor

   Rosalind Segal

   Article

   Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

   Insert

   SFPQ-Y527A (SFPQ Human)
   

   Use

   Lentiviral

   Tags

   Halo

   Expression

   Mutation

   SFPQ-Y527A

   Promoter

   UbC

   Available since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. KLC1N343S-Myc

   Plasmid

   #166963

   Purpose

   Expresses Myc-tagged KLC1 N343S protein in pcDNA3.1

   Depositor

   Rosalind Segal

   Article

   Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

   Insert

   KLC1-N343S (Klc1 Mouse)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   KLC1-N343S

   Promoter

   CMV

   Available since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pETM33_Nsp3c_SUD-C

   Plasmid

   #156471

   Purpose

   Bacterial expression of Sars-CoV2 Nsp3c_SUD-C protein with His-tag and GST-tag

   Depositor

   Ylva Ivarsson

   Article

   Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

   Insert

   Nsp3c_SUD-C (ORF1ab Synthetic)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Gene insert is codon optimized for Ecoli.

   Promoter

   T7/LacO

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pETM33_Nsp3e_NAB

   Plasmid

   #156473

   Purpose

   Bacterial expression of Sars-CoV2 Nsp3e_NAB protein with His-tag and GST-tag

   Depositor

   Ylva Ivarsson

   Article

   Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

   Insert

   Nsp3e_NAB (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Gene insert is codon optimized for Ecoli.

   Promoter

   T7/LacO

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCC_11 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dxCas9-NLS-2A-Puro-WPRE

   Plasmid

   #139096

   Purpose

   Expresses human codon-optimized inactive xCas9 3.7 fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

   Depositor

   Neville Sanjana

   Article

   Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

   Insert

   KRAB-dxCas9 3.7
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A, A262T, R324L,S409I, E480K, E543D, M6…

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. DLG3_PDZ_2

   Plasmid

   #103947

   Purpose

   Protein expression and purification of NE-DLG PDZ2 domain

   Depositor

   Sachdev Sidhu

   Article

   Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

   Insert

   DLG3 (DLG3 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn-GRAB_NE1m

   Plasmid

   #123308

   Purpose

   Expresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m in neurons

   Depositor

   Yulong Li

   Article

   Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d

   Has Service

   AAV Retrograde and AAV9

   Insert

   GPCR activation based NE sensor GRAB_NE1m
   

   Use

   AAV

   Tags

   Expression

   Mutation

   contains a glycine-to-threonine mutation at posit…

   Promoter

   hSyn

   Available since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-hACE2-hygro

   Plasmid

   #161758

   Purpose

   Expresses human ACE2 in mammalian cells

   Depositor

   Neville Sanjana

   Article

   Daniloski et al Cell. 2020 Oct 24. pii: S0092-8674(20)31

   Insert

   Human ACE2 (ACE2 Human)
   

   Use

   Lentiviral

   Tags

   P2A

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Nov. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pHR_PGK-hACE2

   Plasmid

   #161612

   Purpose

   Expression of human ACE2 in mammalian cells

   Depositor

   Brad Rosenberg

   Article

   Daniloski et al Cell. 2020 Oct 24. pii: S0092-8674(20)31

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOT_4 - lenti-EFS-FMC6.3-BBz-2A-puro-2A-LTBR

    Plasmid

    #181973

    Purpose

    Expresses anti-CD19 CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A and to human LTBR via T2A for lentiviral delivery.

    Depositor

    Neville Sanjana

    Article

    Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

    Insert

    FMC6.3-BBz CAR; LTBR (LTBR Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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