We narrowed to 2,385 results for: LARGE;

1. pB_Tet-Off_Flex_DEST_EFS_mODC_tTA (JDW 936)

   Plasmid

   #229828

   Purpose

   A Piggybac compatible, cre dependent, Tet-off destination vector with a downstream WPRE. EFS driving destabilized tTA

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pME Lamin B Vhh mNeonGreen-pA (JDW 1312)

   Plasmid

   #224501

   Purpose

   Gateway compatible middle entry clone containing Lamin B nanobody fused to mNeonGreen with HA Tag (For visualizing the nuclear lamina, lamin chromobody)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   Lamin B Vhh mNeonGreen-pA
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. JDW 1475 (pLenti-BLAST-GATA4-P2A-H2A-mCherry)

   Plasmid

   #233549

   Purpose

   A CMV driven human GATA4 followed by a P2A cleavage peptide and an H2A mCherry fusion protein.

   Depositor

   Joshua Wythe

   Article

   Cantu Gutierrez et al bioRxiv 2021.11.15.468651

   Insert

   GATA4 (GATA4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. p5E-dr-myl7-pro (JDW 1148)

   Plasmid

   #229836

   Purpose

   A gateway compatible 5' entry clone containing the mysoin light chain seven (myl7/cmlc2) promoter for cardiomyocyte specific expression.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   cmlc2/myl7
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pME-DreERT2 (JDW 1344)

   Plasmid

   #224523

   Purpose

   A Gateway-compatible middle entry clone containing Dre recombinase fused to mutant ERT2 for tamoxifen-inducible recombinase activity

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   DreERT2
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pME-Sun1-2xsfGFP-6xMYC-pA (JDW 681)

   Plasmid

   #224489

   Purpose

   Gateway compatible middle entry clone containing Sun1 reporter fusion to 2 copies of sfGFP and 6 MYC tags (Nuclear envelope reporter and affinity tag)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   Sun1-2xsfGFP-6xMYC-pA
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pME Actin-Vhh sfGFP (JDW 1215)

   Plasmid

   #224495

   Purpose

   Gateway compatible middle entry clone with Actin nanobody fused to sfGFP (GFP actin reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   Actin-Vhh-sfGFP
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pME-3xNLS-mTagBFP2-Cre (JDW 918)

   Plasmid

   #224517

   Purpose

   A Gateway-compatible middle entry clone containing a 3xNLS-mTagBFP2-Cre fusion

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   3xNLS-mTagBFP2-Cre
   

   Use

   Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CMV-T7-hOsa2

   Plasmid

   #17987

   Depositor

   Naoko Tanese

   Article

   Inoue et al J Biol Chem. 2002 Nov 1. 277(44):41674-8

   Insert

   Osa2 (ARID1B Human)
   

   Use

   Tags

   T7 epitope

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 19, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pME Actin Vhh mNeonGreen-HA-pA (JDW 1248)

    Plasmid

    #224500

    Purpose

    Gateway compatible middle entry clone containing Actin nanobody fused to mNeonGreen with an HA tag (Visualizing actin, actin chromobody)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    Actin-Vhh-mNeonGreen-HA-pA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pME-EGFP-P2A-iCre (JDW 921)

    Plasmid

    #224519

    Purpose

    A Gateway compatible middle entry clone containing EGFP P2A iCre (codon optimized cre)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    EGFP-P2A-iCre
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. p3E-IRES-3xNLS-mScarlet-I3-pA (JDW 1360)

    Plasmid

    #224536

    Purpose

    A Gateway compatible 3' entry clone containing IRES-FLAG- NLS-mScarlet-I3

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    IRES-FLAG- NLS-mScarlet-I3
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pME-mTurquoise-P2A-iCre (JDW 920)

    Plasmid

    #224518

    Purpose

    A Gateway compatible middle entry clone containing mTurquoise P2A iCre (codon optimized Cre)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    mTurquoise-P2A-iCre
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pME-lox-3xSTOP-lox (JDW 869)

    Plasmid

    #224521

    Purpose

    A Gateway compatible middle entry clone containing a loxP flanked SV40 polyA, bGH polyA, and SV40 pA stop cassette

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    LoxP-3xStop-LoxP
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pME-mScarlet-I-P2A-iCre (JDW 917)

    Plasmid

    #224516

    Purpose

    A Gateway-compatible middle entry clone containing mScarlet-I P2A-iCre (codon optimized)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    mScarlet-I-P2A-iCre
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pME-Lifeact-EGFP-3xHA (JDW 1322)

    Plasmid

    #224494

    Purpose

    Gateway compatible middle entry clone containing Lifeact-EGFP (EGFP F-actin reporter)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    Liefact-EGFP-3xHA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pME-3xNLS-(G2SG2)2-Cre (JDW 1149)

    Plasmid

    #224514

    Purpose

    A Gateway compatible middle entry clone containing a 3x nuclear localization signal followed by a multiple cloning site and a flexible linker fused to Cre

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    3xNLS-(G2SG2)2-Cre
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p5E-TetO8x-CMVmin (JDW 1087)

    Plasmid

    #224475

    Purpose

    Gateway compatible 5' entry clone containing 8 tet operons (TREs) upstream of a minimal CMV promoter

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    TetO(8x)-CMV promoter
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CMV-T7-hOsa1

    Plasmid

    #17986

    Depositor

    Naoko Tanese

    Article

    Inoue et al J Biol Chem. 2002 Nov 1. 277(44):41674-8

    Insert

    Osa1 (ARID1A Human)
    

    Use

    Tags

    T7 epitope

    Expression

    Mammalian

    Mutation

    Missing 5' end

    Promoter

    Available since

    Aug. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

20. myc-POMP

    Plasmid

    #86764

    Purpose

    N-terminal myc-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

    Insert

    human POMP (POMP Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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