We narrowed to 2,463 results for: T2A

1. pMSCVpuro-Gcn5(D608A)

   Plasmid

   #63707

   Purpose

   retroviral expression of enzyme-dead mouse Gcn5 in mammalian cells

   Depositor

   Kai Ge

   Article

   Jin et al EMBO Rep. 2014 Nov;15(11):1192-201. doi:

   Insert

   Gcn5 (Kat2a Mouse)
   

   Use

   Retroviral

   Tags

   None

   Expression

   Mammalian

   Mutation

   D608A mutation

   Available Since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

2. HTR2A-DuET

   Plasmid

   #213321

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   HTR2A (HTR2A Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT22iU66CriT(#387)

   Plasmid

   #184061

   Purpose

   cyclofen-inducible CRE activation in zebrafish permanent transgenic

   Depositor

   Sophie Vriz

   Article

   Sinha et al Zebrafish. 2010 Jun;7(2):199-204. doi: 1

   Insert

   CRE-ERT2
   

   Use

   Zebrafish transgenesis (blue eyes)

   Mutation

   ERT2: G400V, M543A, L544A, deleted 1-281;

   Promoter

   Zf-Ubi

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Kif1bβ-pcDNA3

   Plasmid

   #22534

   Depositor

   William Kaelin

   Article

   Schlisio et al Genes Dev. 2008 Apr 1. 22(7):884-93.

   Insert

   Kif1bβ (KIF1B Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   wt cDNA

   Available Since

   Dec. 3, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1(+)-FLAG-RGS14

   Plasmid

   #236567

   Purpose

   Expresses Flag-RGS14 in mammalian cells

   Depositor

   John Hepler

   Article

   Friedman et al J Biol Chem. 2022 May;298(5):101836. doi

   Insert

   Homo sapiens regulator of G-protein signaling 14 (RGS14 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSCVpuro-F-Gcn5-NLSm

   Plasmid

   #63708

   Purpose

   retroviral expression of cytoplasm-localized mouse Gcn5 in mammalian cells

   Depositor

   Kai Ge

   Article

   Jin et al EMBO Rep. 2014 Nov;15(11):1192-201. doi:

   Insert

   Gcn5 (Kat2a Mouse)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   mutated K425, K427 and K438 to R (arginine)

   Available Since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pT22gcfUbm5En25EE4PCh(#552)

   Plasmid

   #184092

   Purpose

   cyclofen-inducible chicken EN2 (mut) activation in zebrafish permanent transgenic

   Depositor

   Sophie Vriz

   Article

   Amblard et al Commun Biol. 2020 Sep 29;3(1):536. doi:

   Insert

   5myc-chkEn2(5E)-ERT2-P2A-mCherry
   

   Use

   Zebrafish transgenesis (blue eyes)

   Tags

   5xMyc

   Mutation

   chkEn2: S150E, S151E, S153E, S155E, S156E; ERT2: …

   Promoter

   Zf-Ubi

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT22Ubm4Eng2bKRPC(#493)

   Plasmid

   #184091

   Purpose

   cyclofen-inducible fish Eng2b (mut) activation in zebrafish permanent transgenic

   Depositor

   Sophie Vriz

   Article

   Amblard et al Commun Biol. 2020 Sep 29;3(1):536. doi:

   Insert

   4myc-ZfEng2b(WW>KK)-ERT2-P2A-mCherry
   

   Use

   Zebrafish transgenesis (blue eyes)

   Tags

   4xMyc

   Mutation

   ZfEng2b: W141K, W144K; ERT2: G400V, M543A, L544A,…

   Promoter

   Zf-Ubi

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT22UbGfpnE4PCh(#301)

   Plasmid

   #184066

   Purpose

   cyclofen-inducible GFP nuclear relocation in zebrafish permanent transgenic

   Depositor

   Sophie Vriz

   Article

   Zhang et al Chembiochem. 2018 Jun 18;19(12):1232-123

   Insert

   eGFP-nls-ERT2-P2A-mCherry
   

   Use

   Zebrafish transgenesis (blue eyes)

   Mutation

   ERT2: G400V, M543A, L544A, deleted 1-281;

   Promoter

   Zf-Ubi

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOT_6 - lenti-EFS-FMC6.3-BBz-2A-puro-2A-tNGFR

    Plasmid

    #181975

    Purpose

    Expresses anti-CD19 CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A and to human truncated NGFR via T2A for lentiviral delivery.

    Depositor

    Neville Sanjana

    Article

    Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

    Insert

    FMC6.3-BBz CAR; tNGFR (NGFR Human, Synthetic)
    

    Use

    Lentiviral

    Mutation

    NGFR truncation: aa 11-276

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOT_5 - lenti-EFS-FMC6.3-28z-2A-puro-2A-tNGFR

    Plasmid

    #181974

    Purpose

    Expresses anti-CD19 CAR (with CD28 signaling domain) linked to puromycin resistance via P2A and to human truncated NGFR via T2A for lentiviral delivery.

    Depositor

    Neville Sanjana

    Article

    Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

    Insert

    FMC6.3-28z CAR; tNGFR (NGFR Human, Synthetic)
    

    Use

    Lentiviral

    Mutation

    NGFR truncation: aa 11-276

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV_U6-ωRNA*_ISDra2-TnpB-mNG

    Plasmid

    #212968

    Purpose

    AAV vector for encoding an ISDra2-TnpB driven by EFs promoter

    Depositor

    Chunlong Xu

    Article

    Li et al Nat Commun. 2024 Jan 27;15(1):831. doi:

    Inserts

    ISDra2-TnpB-T2A-mNG,
    ωRNA*
    

    Use

    AAV and CRISPR

    Tags

    NLS and NLS-FLAG

    Expression

    Mammalian

    Promoter

    EF1a, T7 and U6

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHAGE2-TetOminiCMV-OSK

    Plasmid

    #136552

    Purpose

    Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Oct4, Sox2, Klf4

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    Oct4-P2A-Sox2-T2A-Klf4 (Klf4, Sox2, Pou5f1 Mouse)
    

    Use

    Lentiviral

    Expression

    Bacterial

    Promoter

    tetO-miniCMV (dox-inducible)

    Available Since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. ISDra2-TnpB-mCherry-U6-ωRNA*

    Plasmid

    #212967

    Purpose

    Expression vector for expression of ISDra2-TnpB driven by chicken β-actin promoter and mCherry driven by CMV promoter

    Depositor

    Chunlong Xu

    Article

    Li et al Nat Commun. 2024 Jan 27;15(1):831. doi:

    Inserts

    ISDra2-TnpB-T2A-mCherry
    ωRNA*
    

    Use

    CRISPR

    Tags

    NLS and NLS-FLAG

    Expression

    Mammalian

    Promoter

    CAG, T7 and U6

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAVS1 CAG rtTA3 TauP301L 2N4R-EGFP

    Plasmid

    #132393

    Purpose

    Exchange cassette containing inducible TauP301L 2N4R-EGFP fusion, CAG-driven rtTA3, Puromycin resistance marker, targets AAVS1 site

    Depositor

    Gerold Schmitt-Ulms

    Article

    Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

    Insert

    human TauP301L 2N4R-EGFP (MAPT Human, Synthetic)
    

    Use

    CRISPR and Cre/Lox

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Proline 301 to Leucine (P301L)

    Promoter

    TREtight

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. MIP 339 CoMiP coLin28 2A Nanog IRES eGFP

    Plasmid

    #63729

    Purpose

    To express codon optimized Lin-28 and wild type Nanog. A single plasmid reprogramming system using a mini-intronic plasmid (MIP).

    Depositor

    Joseph Wu, Mark Kay

    Article

    Diecke et al Sci Rep. 2015 Jan 28;5:8081. doi: 10.103

    Inserts

    Lin28 (LIN28A Human)
    Nanog (NANOG Human)
    

    Tags

    T2A

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    SFFV

    Available Since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCRISPaint-mScarlet-PuroR [M1G]

    Plasmid

    #171809

    Purpose

    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein and puromycin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mScarlet-T2A-PuroR
    

    Use

    Gene tagging

    Mutation

    PuroR: Changed Methionin 1 to Glycine.

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCRISPaint-mScarlet-BlastR [M1G]

    Plasmid

    #171814

    Purpose

    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein and blasticidin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mScarlet-T2A-BlastR
    

    Use

    Gene tagging

    Mutation

    BlastR: Changed Methionin 1 to Glycine.

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHAGE2-TetOminiCMV-SKM

    Plasmid

    #136551

    Purpose

    Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Sox2, Klf4, cMyc

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    Sox2-T2A-Klf4-E2A-cMyc (Sox2, Klf4, Myc Mouse)
    

    Use

    Lentiviral

    Expression

    Bacterial

    Promoter

    tetO-miniCMV (dox-inducible)

    Available Since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCRISPaint-mNeon-PuroR [M1G]

    Plasmid

    #171800

    Purpose

    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein and puromycin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mNeonGreen-T2A-PuroR
    

    Use

    Gene tagging

    Mutation

    PuroR: Changed Methionin 1 to Glycine.

    Available Since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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