Addgene

We narrowed to 8,581 results for: ef1

Showing: 2201 - 2220 of 8581 results

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PWPXLD-GFP-Numb-WT

Plasmid

#133751

Purpose

Wild-type Numb tagged with GFP cloned into PWPXLD

Depositor

Chay Kuo

Article

Abdi et al Cell Rep. 2019 Aug 20;28(8):2012-2022.e4

Insert

GFP-Numb (Numb Mouse)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

EF1-alpha

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAT9748-dCBE

Plasmid

#163008

Purpose

plasmid expressing CBE with dCas9

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

dCBE

Use

CRISPR

Tags

3xFLAG and SV40 NLS

Expression

Mammalian

Mutation

Promoter

EF1-a

Available since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pAS_4xMma PylT_GFP 150TAG 6xHis

Plasmid

#140015

Purpose

Plasmid with 4xMmaPylT cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

sfGFP

Use

Tags

6xHis

Expression

Mammalian

Mutation

150TAG

Promoter

EF1

Available since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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Flag-mLmo2

Plasmid

#64893

Purpose

Lentiviral expression of Flag-tagged murine transcription factor Lmo2

Depositor

Georges Lacaud

Article

Batta et al Cell Rep. 2014 Dec 11;9(5):1871-84. doi:

Insert

Lmo2 (Lmo2 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 5, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti-FNLSNG-P2A-Puro

Plasmid

#136900

Purpose

Lentivirus for constitutive expression of FNLSNG in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

Insert

FNLSNG

Use

Lentiviral

Tags

FLAG-NLS and NLS

Expression

Mutation

L1111R,D1135V,G1218R,E1219F,A1322R,R1335V,T1337R

Promoter

EF1s

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCDH_Str-Ii

Plasmid

#65313

Purpose

Cytoplasmic ER hook only (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Invariant chain (p33)

Use

Lentiviral

Tags

Expression

Mutation

Streptavidin in its cytoplasmic part

Promoter

CMV

Available since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pCDH-CRY2-mCh-superPLDx10

Plasmid

#188986

Purpose

A lentiviral plasmid encoding CRY2-mCh-superPLDx10 (an engineered PLD mutant with 10 times higher activity than the wild-type)

Depositor

Jeremy Baskin

Article

Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

Insert

PLD

Use

Tags

CRY2 and mCherry

Expression

Mammalian

Mutation

F163L, K249R, G328S, G381V, G429D, D480G

Promoter

CMV

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAS_FLAG-Mma PylRS

Plasmid

#154762

Purpose

plasmid with Mma PylRS for amber suppression in transient or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

Mma PylRS (pylS Methanosarcina mazei)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

EF1

Available since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pJAM1.16_PB-Cx43_S368A-PGK-Blast

Plasmid

#205561

Purpose

Express human Cx43 with S368A mutation

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

Cx43

Use

Piggybac

Tags

Expression

Mammalian

Mutation

S368A

Promoter

EF1

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-FNLSHiFiNG-P2A-Puro

Plasmid

#136903

Purpose

Lentivirus for constitutive expression of FNLSHiFiNG in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

Insert

FNLSHiFiNG

Use

Lentiviral

Tags

FLAG-NLS and NLS

Expression

Mutation

R691A,L1111R,D1135V,G1218R,E1219F,A1322R,R1335V,T…

Promoter

EF1s

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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FL-BCAN-lentiviral-GFP

Plasmid

#29704

Depositor

Mariano Viapiano

Article

Hu et al Mol Cancer Res. 2009 Nov . 7(11):1756-70

Insert

Brevican (BCAN Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only
sc AAV miR26a eGFP

Plasmid

#21894

Depositor

Joshua Mendell

Article

Kota et al Cell. 2009 Jun 12. 137(6):1005-17.

Insert

EF1 alpha -miR26a GFP p(A)

Use

AAV; Sc

Tags

GFP

Expression

Mammalian

Mutation

N/A

Promoter

Available since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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pEF-ENTR-LacZ (710-1)

Plasmid

#17430

Purpose

Gateway entry vector with EF1-a promoter, V5-His C-term, Lac Z

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

LacZ

Use

Entry vector

Tags

His and V5

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pPBT-peRNA_GG-Puro

Plasmid

#173220

Purpose

piggyBac transposon containing hU6 promotor and pegRNA cloning site

Depositor

Jacob Giehm Mikkelsen

Article

Wolff et al Front Genome Ed. 2021 Nov 12;3:786893. d

Inserts

pegRNA cloning cassette
PuroR

Use

Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

EF1-a and hU6

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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PM PKA Reporter

Plasmid

#104551

Purpose

Expresses Lyn-mTurquoise2-VASP at the plasma membrane of mammalian cells

Depositor

David Lawrence

Article

O'Banion et al Cell Chem Biol. 2017 Oct 25. pii: S2451-

Insert

Lyn-mTurquoise2-VASP 148 - 164

Use

Tags

mTurquoise 2, HA tag

Expression

Mammalian

Mutation

Promoter

EF1 alpha

Available since

March 6, 2018

Availability

Academic Institutions and Nonprofits only

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pDBEcR/Bla

Plasmid

#201556

Purpose

Plasmid with SB ITRs for expressing DBEcR (3xmyc-tagged) under EF1α promoter; blasticidin resistance.

Depositor

Randy Poon

Article

Yeung et al J Cell Sci. 2023 Apr 15;136(8):jcs260754

Insert

DBEcR

Use

Unspecified; Sleeping beauty transposon

Tags

Myc

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 13, 2023

Availability

Academic Institutions and Nonprofits only

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OMM PKA Reporter

Plasmid

#104550

Purpose

Expresses Tom20-mTurquoise2-VASP at the outer mitochondrial membrane of mammalian cells.

Depositor

David Lawrence

Article

O'Banion et al Cell Chem Biol. 2017 Oct 25. pii: S2451-

Insert

Tom20 - mTurquoise2 - VASP 148-164

Use

Tags

mTurqouise2

Expression

Mammalian

Mutation

Promoter

EF1 alpha

Available since

March 6, 2018

Availability

Academic Institutions and Nonprofits only

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pEF-hSEMA4C-Fc

Plasmid

#74449

Purpose

Expresses human Semaphorin-4C ectodomain fused to human Fc in mammalian cells

Depositor

Roland Friedel

Article

Le et al Oncotarget. 2015 Mar 30;6(9):7293-304.

Insert

human Semaphorin-4C ectodomain with human Fc tag (SEMA4C Human)

Use

Tags

human Fc tag

Expression

Mammalian

Mutation

Promoter

EF1-alpha

Available since

May 2, 2016

Availability

Academic Institutions and Nonprofits only

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pAT15485_Hypa-ABE8e

Plasmid

#174123

Purpose

Expresses ABE8e fused with Hypa-SpCas9

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

Hypa-ABE8e

Use

CRISPR

Tags

Nucleoplasmin NLS and SV40 NLS

Expression

Mammalian

Mutation

Promoter

EF1-a

Available since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pAS_4xG1 PylT sfGFP 150TAG

Plasmid

#154767

Purpose

plasmid with 4xG1 PylT cassette and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

sfGFP

Use

Tags

Expression

Mammalian

Mutation

150TAG

Promoter

EF1

Available since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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