We narrowed to 16,981 results for: tes

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plko-shmfn2-mito-GFP-ER tethering

Plasmid

#160509

Purpose

Rescue mfn2 knockdown by tethering mitochondria and ER with GFP

Depositor

Qing Deng

Article

Zhou et al J Cell Sci. 2020 Sep 4;133(17). pii: jcs

Insert

mito-GFP-ER tethering

Use

Lentiviral

Promoter

CMV

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1 3xFlag-CREB

Plasmid

#82721

Purpose

Expresses human CREB

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

CREB (CREB1 Human)

Tags

3XFLAG and GST

Expression

Bacterial

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-eEF2K

Plasmid

#110160

Purpose

Expression of human eEF2K in mammalian cells

Depositor

Daniele Guardavaccaro

Article

Kruiswijk et al Sci Signal. 2012 Jun 5;5(227):ra40. doi:

Insert

eEF2K (eukaryotic elongation factor 2 kinase) (EEF2K Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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pSYC-97

Plasmid

#31565

Depositor

Seok-Yong Choi

Article

Kim et al PLoS One. 2011 . 6(4):e18556.

Insert

pCS4+-NLS-EGFP-P2A-mCherry-CAAX

Available Since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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AAV-GFAP-NLS-CasRx-NLS-FLAG

Plasmid

#154000

Purpose

Express CasRx for AAV package

Depositor

Hui Yang

Article

Zhou et al Cell. 2020 Apr 30;181(3):590-603.e16. do

Insert

CasRx

Use

AAV and CRISPR

Tags

FLAG

Promoter

GFAP

Available Since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1.shCDK8

Plasmid

#19760

Depositor

William Hahn

Article

Firestein et al Nature. 2008 Sep 25. 455(7212):547-51.

Insert

small hairpin RNA against CDK8 (CDK8 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
AICSDP-49: MYL2-mEGFP

Plasmid

#114414

Purpose

Homology arms and linker-mEGFP sequence for C-terminus tagging of human MYL2, via Cas9-excisable CAGGS-mCherry selection cassette

Depositor

Allen Institute for Cell Science

Article

Roberts et al Stem Cell Reports. 2019 May 14;12(5):114

Insert

MYL2 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (MYL2 Human)

Use

CRISPR; Donor template

Tags

linker-mEGFP

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pTKDP-dhfr

Plasmid

#71323

Purpose

Donor plasmid - trimethoprim resistant donor cassette

Depositor

Thomas Kuhlman

Article

Tas et al PLoS One. 2015 Sep 2;10(9):e0136963. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-Tollip

Plasmid

#178050

Purpose

GFP fused to human Tollip cDNA. Localisation and expression in mammalian cells.

Depositor

David Tumbarello

Article

Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi:

Insert

Toll-Interacting Protein (TOLLIP Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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mito-mScarlet

Plasmid

#141153

Purpose

Fluorescent labeling of the mitochondrial matrix in mammalian cells

Depositor

Gia Voeltz

Article

Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

COX4 (COX4 Budding Yeast)

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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Tet-myr-GFP

Plasmid

#83468

Purpose

Lentiviral vector for doxycycline-inducible expression of membrane targeted-GFP in mammalian cells

Depositor

Barry Gumbiner

Article

Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d

Insert

Myristoylated GFP

Use

Lentiviral

Expression

Mammalian

Promoter

doxycycline-inducible promoter

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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UBXD1-GFP

Plasmid

#86464

Purpose

Expression of human UBXD1 with C-terminal GFP tag

Depositor

Hemmo Meyer

Article

Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

Insert

UBXD1 (UBXN6 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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mRIP3 GFP D161N

Plasmid

#41383

Depositor

Francis Chan

Article

Cho et al Cell. 2009 Jun 12;137(6):1112-23.

Insert

RIP3 (Ripk3 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

Kinase Domain Mutant (D161N)

Promoter

CMV

Available Since

Dec. 4, 2012

Availability

Academic Institutions and Nonprofits only
pCSC-SP-PW-NepX (aka: pBOB-NEPX)

Plasmid

#12340

Depositor

Inder Verma

Article

Marr et al J Mol Neurosci. 2004 . 22(1-2):5-11.

Insert

NEP-X (MME Human)

Use

Lentiviral

Expression

Mammalian

Mutation

E585V (inactive mutant)

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_ 2xFLAG-2xSTREP_NRF2

Plasmid

#159129

Purpose

expresses NRF2 in mammalian cells

Depositor

Michele Pagano

Article

Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

Insert

NRF2 (NFE2L2 Human)

Tags

2X-FLAG _ 2X-STREP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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Px461-Cas9n-Trp53-sgRNA-alpha

Plasmid

#88846

Purpose

CRISPR KO of Trp53

Depositor

Joan Massague

Article

Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

Insert

Trp53 (Trp53 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

July 11, 2017

Availability

Academic Institutions and Nonprofits only
Lenti-EFS-BirAopt-GSQ-RNF4wt-P2A-BLAST

Plasmid

#208046

Purpose

Enables constitutive expression of N-terminal BirAopt-fused RNF4wt; to perform bioE3; selection with blasticidin

Depositor

Rosa Barrio, James Sutherland

Article

Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

Insert

RNF4 (RNF4 Human)

Use

Lentiviral

Tags

BirAopt

Promoter

EFS

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pLV 4xSTAR-sTomato-NLS-blast

Plasmid

#136259

Purpose

Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Depositor

Hugo Snippert

Article

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

stem cell Ascl2 reporter, 4 repeats

Use

Lentiviral

Tags

sTomato-NLS

Expression

Mammalian

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-BaLgp160

Plasmid

#100919

Purpose

Mammalian expression plasmid for Env from the BaL HIV-1 isolate

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (BaL) Env

Tags

CD5 leader sequence

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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pT7-7 asyn G51D

Plasmid

#105747

Purpose

Bacterial expression of mutant human alpha synuclein

Depositor

Hilal Lashuel

Article

Fares et al Hum Mol Genet. 2014 Sep 1;23(17):4491-50

Insert

alpha-synuclein G51D (SNCA Human)

Expression

Bacterial

Mutation

G51D

Promoter

T7

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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