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Plasmid#128755PurposeExpresses CYRANO gRNA for CRISPRiDepositorInsertCYRANO sgRNA (OIP5-AS1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermU6Available sinceAug. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA EF1Alpha-puro-T2A-BFP
Plasmid#60955PurposeParental vector for the CRISPRi/a libraries. Expresses an sgRNA from the U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoterDepositorInsertssgGFP-NT2
puro-T2A-BFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
EF1a-LCB1_v1.3-PDGFR-T2A-mCherry (pZYW048)
Plasmid#194231PurposeLentiviral expression vector expressing LCB1 on the cell surface attached to PDGFR's transmembrane domain and mCherry transduction markerDepositorInsertEF1a-LCB1-PDGFR TMD-T2A-mCherry
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
U6-sgRNA(MS2)_EF1a-MS2-P65-HSF1
Plasmid#92120PurposeExpression plasmid for both MS2-P65-HSF1 activator helper complex and sgRNA with two MS2 loops at tetraloop and stemloop 2 contains BsaI sites for insertion of spacer sequences.DepositorInsertMS2-P65-HSF1 (HSF1 Human, Synthetic)
UseCRISPRTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-GFP-LaminA-IRES-Hygromycin
Plasmid#134867PurposeExpresses EGFP-tagged nuclear envelope markerDepositorInsertLamin A (LMNA Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-RAB7A-IRES-Puromycin
Plasmid#133027PurposeExpresses EGFP-tagged late endosome markerDepositorInsertRAB7A (RAB7A Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterHuman elongation factor 1 alphaAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-RAB5A-IRES-Puromycin
Plasmid#134858PurposeExpresses EGFP-tagged early endosome markerDepositorInsertRas-related protein 5A
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-mCherry-P2A-NLSFlpN-nMagHigh
Plasmid#180585PurposePA-FlpDepositorInsertSplit FlpO
UseAAVTagsExpressionMutationPromoterAvailable sinceFeb. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-EF1a-KRAB-dCas9-P2A-EGFP
Plasmid#118156PurposeCatalytically inactive Cas9 from S. pyogenes with P2A-EGFP under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.DepositorInsertKRAB-dCas9-P2A-EGFP
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterEF1a core and U6Available sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO-ChETA-TdTomato-WPRE-pA
Plasmid#37755PurposeCre-dependent expression of ChETA-tdTomato, for optogenetic excitationDepositorInsertChETA-TdTomato
UseAAV and Cre/Lox; Cre-onTagsTdTomatoExpressionMutationPromoterEF1aAvailable sinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only