We narrowed to 4,627 results for: pcr

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e-ciCas9_pcDNA5

Plasmid

#100553

Purpose

Expresses enhanced specificity ciCas9 (e-ciCas9) in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.

Depositor

Dustin Maly

Article

Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

Insert

e-ciCas9

Use

CRISPR; Frt, flp-in

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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CrBlue ---- pcDNA3.1

Plasmid

#62917

Purpose

CrBlue is a synthetic chimera comprising the blue opsin backbone with the intracellular loops and the C terminus of the jellyfish opsin, created to introduce spectral selectivity.

Depositor

Narasimhan Gautam

Article

Karunarathne et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

Insert

CrBlue

Expression

Mammalian

Promoter

CMV

Available Since

April 13, 2015

Availability

Academic Institutions and Nonprofits only
Salsa-BC15-P1B3

Plasmid

#202856

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC15

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC15

Expression

Mammalian

Promoter

Tet Response Element (TRE)

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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Salsa-BC11-P1A11

Plasmid

#202852

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC11

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC11

Expression

Mammalian

Promoter

Tet Response Element (TRE)

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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ciCas9(L22)_pcDNA5

Plasmid

#100551

Purpose

Expresses ciCas9(L22) in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.

Depositor

Dustin Maly

Article

Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

Insert

ciCas9(L22)

Use

CRISPR; Frt, flp-in

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pNEXdelta-EGFP-ApAF

Plasmid

#13335

Depositor

Bong-Kiun Kaang

Article

Lee et al J Cell Biol. 2006 Sep 11. 174(6):827-38.

Insert

Aplysia activating factor

Tags

EGFP

Available Since

Nov. 17, 2006

Availability

Academic Institutions and Nonprofits only
ciCas9(F22)_pcDNA5

Plasmid

#100552

Purpose

Expresses ciCas9(F22) in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.

Depositor

Dustin Maly

Article

Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

Insert

ciCas9(F22)

Use

CRISPR; Frt, flp-in

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pNEXdelta-ApAF

Plasmid

#13334

Depositor

Bong-Kiun Kaang

Article

Lee et al J Cell Biol. 2006 Sep 11. 174(6):827-38.

Insert

Aplysia activating factor

Available Since

Nov. 17, 2006

Availability

Academic Institutions and Nonprofits only
Salsa-BC82-P1G10

Plasmid

#202923

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC82

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC82

Expression

Mammalian

Promoter

Tet Response Element (TRE)

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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Salsa-BC2-P1A2

Plasmid

#202843

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC2

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC2

Expression

Mammalian

Promoter

Tet Response Element (TRE)

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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Salsa-BC6-P1A6

Plasmid

#202847

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC6

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC6

Expression

Mammalian

Promoter

Tet Response Element (TRE)

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pTL472

Plasmid

#69882

Purpose

dCirl promoter region was replaced with an optimized 20xUAS-IVS promoter cassette

Depositor

Robert Kittel, Tobias Langenhan

Article

Scholz et al Cell Rep. 2015 May 12;11(6):866-74. doi:

Insert

CIRL (Cirl Fly)

Use

Transformation vector

Tags

FLAG

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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BtARR2-3A

Plasmid

#245789

Purpose

Expression of mutated bovine arrestin-2 (I386A, V387A, F388A) in bacteria

Depositor

Vsevolod Gurevich

Article

Zheng et al Cells. 2023 Jun 6;12(12):1563. doi: 10.3

Insert

arrestin-2 (ARRB2 Bovine)

Expression

Bacterial

Mutation

I386A, V387A, F388A

Promoter

lac

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pSmBtA3

Plasmid

#245782

Purpose

Expression of bovine arrestin-3 tagged with SmBiT in mammalian cells

Depositor

Vsevolod Gurevich

Article

Zheng et al Cells. 2023 Jun 6;12(12):1563. doi: 10.3

Insert

arrestin-3 (ARR3 Bovine)

Tags

SmBiT

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pVEA3-DR

Plasmid

#234800

Purpose

Mammalian expression of bovine arrestin-3 (D291R)

Depositor

Vsevolod Gurevich

Article

Zheng et al Cells. 2023 Jun 6;12(12):1563. doi: 10.3

Insert

Arrestin-3 (ARR3 Bovine)

Tags

Venus

Expression

Mammalian

Mutation

D291R

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pVEA3-Tr

Plasmid

#234798

Purpose

Mammalian expression of truncated bovine arrestin-3 (1-393)

Depositor

Vsevolod Gurevich

Article

Zheng et al Cells. 2023 Jun 6;12(12):1563. doi: 10.3

Insert

Arrestin-3 (ARR3 Bovine)

Tags

Venus

Expression

Mammalian

Mutation

truncated (1-393)

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pVEA3-RE

Plasmid

#234799

Purpose

Mammalian expression of bovine arrestin-3 (R170E)

Depositor

Vsevolod Gurevich

Article

Zheng et al Cells. 2023 Jun 6;12(12):1563. doi: 10.3

Insert

Arrestin-3 (ARR3 Bovine)

Tags

Venus

Expression

Mammalian

Mutation

R170E

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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Salsa-BC293-P4B1

Plasmid

#203134

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC293

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC293

Expression

Mammalian

Promoter

Tet Response Element (TRE)

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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Salsa-BC170-P2G2

Plasmid

#203011

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC170

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC170

Expression

Mammalian

Promoter

Tet Response Element (TRE)

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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Salsa-BC157-P2F1

Plasmid

#202998

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC157

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC157

Expression

Mammalian

Promoter

Tet Response Element (TRE)

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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