We narrowed to 4,881 results for: phy

1. Human PNP his-tagged

   Plasmid

   #64076

   Purpose

   Expression of human Purine Nucleoside Phosphorylase

   Depositor

   Vern Schramm

   Article

   Lewandowicz et al J Biol Chem. 2003 Aug 22;278(34):31465-8

   Insert

   Purine Nucleoside Phosphorylase (PNP Human)
   

   Use

   Tags

   Histidine tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pBAD-smURFP-RBS-HO-1

   Plasmid

   #80341

   Purpose

   Expresses smURFP and Heme Oxygenase-1 in bacterial cells.

   Depositor

   Roger Tsien, Erik Rodriguez

   Article

   Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

   Inserts

   smURFP
   HO-1
   

   Use

   Tags

   6 Histidine Tag

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pP35S (GB0030)

   Plasmid

   #68163

   Purpose

   Provides the CaMV 35S Promoter as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   CaMV 35S promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Oct. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMF-TetTight

   Plasmid

   #193288

   Purpose

   Lentiviral construct that expresses rtTA-p2a-tTS for non-leaky dox-inducible expression of a transgene

   Depositor

   Rudolf Jaenisch

   Article

   Friesen et al Sci Adv. 2022 Jun 17;8(24):eabn7298. doi

   Insert

   rtTA-P2A-tTS
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF‐1α

   Available since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTnos (GB0037)

   Plasmid

   #68188

   Purpose

   Provides the Agrobacterium tumefaciens Nopaline synthase terminator as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   Nos terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Oct. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRRL_U2AF1_S34F_mCherry

   Plasmid

   #84019

   Purpose

   expression of mutant U2AF1 in mammalian cells

   Depositor

   Robert Bradley

   Article

   Ilagan et al Genome Res. 2015 Jan;25(1):14-26. doi: 1

   Insert

   U2AF1 (U2AF1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG and T2A-mCherry

   Expression

   Mammalian

   Mutation

   S34F

   Promoter

   PGK

   Available since

   Oct. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMOS016: roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)

   Plasmid

   #163058

   Purpose

   roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)

   Depositor

   Adam Cohen

   Article

   Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

   Insert

   roGFP2-Orp1
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pKA-207I10

   Plasmid

   #79845

   Purpose

   Expresses NLS-PpsR2-VP16-IRES-RpBphP1-mCherry-TetR under CMV promoter

   Depositor

   Vladislav Verkhusha

   Article

   Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm

   Insert

   NLS-PpsR2-VP16-IRESI10-BphP1-mCherry-TetR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   RpPpsR2 cysteine 439 changed to serine

   Promoter

   CMV

   Available since

   Aug. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBI-EGFP-MnSOD

   Plasmid

   #16612

   Depositor

   Bert Vogelstein

   Article

   Hwang et al Nat Med. 2001 Oct . 7(10):1111-7.

   Insert

   MnSOD (SOD2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 7, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CROP_C9_Puro_TOP1

    Plasmid

    #183323

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets TOP1 gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    TOP1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CROP_C9_Puro_AAVS1A

    Plasmid

    #183337

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets the AAVS1 locus

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    AAVS1A
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CROP_C9_Puro_AAVS1B

    Plasmid

    #183338

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets the AAVS1 locus

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    AAVS1B
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CROP_C9_Puro_TOP2B

    Plasmid

    #183325

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets TOP2B gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    TOP2B
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CROP_C9_Puro_TOP2A

    Plasmid

    #183324

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets TOP2A gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    TOP2A
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CaVα2δ1

    Plasmid

    #26575

    Depositor

    Diane Lipscombe

    Article

    Lin et al J Neurophysiol. 2004 Nov . 92(5):2820-30

    Insert

    Cacna2d1 (Cacna2d1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pC1-REX-YFP

    Plasmid

    #48247

    Purpose

    Genetically encoded fluorescent indicator for measuring NAD/NADH ratio.

    Depositor

    Vsevolod Belousov

    Article

    Bilan et al Biochim Biophys Acta. 2013 Nov 25. pii:

    Insert

    REX-YFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCDB179-TurboPETase

    Plasmid

    #218693

    Purpose

    Encodes codon optimized TurboPETase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    TurboPETase
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEGFP-C1-Nup35

    Plasmid

    #87342

    Purpose

    To express human Nup35 in mammalian cells

    Depositor

    Lei Lu

    Article

    Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

    Insert

    nucleoporin 35 (NUP35 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. CMV-R-GECO1.2

    Plasmid

    #45494

    Purpose

    Red intensiometric genetically encoded Ca2+-indicators for optical imaging 1.2

    Depositor

    Robert Campbell

    Article

    Wu et al ACS Chem Neurosci. 2013 Mar 19.

    Insert

    R-GECO1.2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R-GECO1 M164R/I166V/V174L/F222L/N267S/S270T/I330M…

    Promoter

    CMV

    Available since

    July 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBMNb-cALFA

    Plasmid

    #159991

    Purpose

    For the protein expression with ALFA tag at C-terminus in mammalian cells

    Depositor

    Motoyuki Hattori

    Article

    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

    Type

    Empty backbone

    Use

    Tags

    HRV3C protease cleavage site-ALFA tag-twin Strep …

    Expression

    Mammalian

    Mutation

    CMV promoter, WPRE

    Promoter

    Available since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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