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pMC8b-XI3up

Plasmid

#176170

Purpose

yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XI-3

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

5' homology for integration site XI-3

Use

Synthetic Biology

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC7-XI3dw

Plasmid

#176171

Purpose

yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XI-3

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

3' homology for integration site XI-3

Use

Synthetic Biology

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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p3E-FRT-KanR-FRT pA

Plasmid

#82601

Purpose

3' entry vector containing FRT-flanked kanamycin resistance cassette (KanR) with pA for FLP-induced kanamycin resistance, drug selection

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

FRT-Kan-FRT

Promoter

None

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pMCL9

Plasmid

#176186

Purpose

2 mu cloning vector for single or multiplexed gRNAs, SNR52p-sfGFP-scRNA-SUP4t-CYC1t

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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mNeongreen-PXN

Plasmid

#129604

Purpose

In vivo visualization of focal adhesions (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Paxillin

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pFW1000

Plasmid

#224210

Purpose

E. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), R6K (E. coli replication origin)

Depositor

Ophelia Venturelli

Article

Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.cytGAP

Plasmid

#78734

Purpose

Expression of Ca2+ sensor GAP in the cytosol

Depositor

Teresa Alonso

Article

Rodriguez-Garcia et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

Insert

cytGAP

Tags

luciferase

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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PylRS-AS

Plasmid

#137908

Purpose

For incorporation of fluorophenylalanine derivatives

Depositor

Wenshe Liu

Article

Lee et al Chem Commun (Camb). 2016 Oct 18;52(85):1

Insert

Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair

Expression

Bacterial

Mutation

Containing N346A, C348S mutations

Promoter

pBAD

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-loxSTOPlox-LynEGFP

Plasmid

#133922

Purpose

Reporter expressing membrane targeted EGFP for visualization of neuronal morphology upon Cre-based recombination

Depositor

Frank Bradke, Sebastián Dupraz

Article

Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

Insert

LynEGFP

Expression

Mammalian

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pEBFP2-Nuc

Plasmid

#14893

Purpose

Mammalian expression of EBFP2 blue fluorescent protein with NLS

Depositor

Robert Campbell

Article

Ai et al Biochemistry. 2007 May 22;46(20):5904-10

Insert

pEBFP2-Nuc

Tags

NLS

Expression

Mammalian

Mutation

Replace EYFP of the vector pEYFP-Nuc from Clontec…

Available Since

May 15, 2007

Availability

Academic Institutions and Nonprofits only

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