178,119 results
-
Plasmid#172666Purposeencodes NL63 spike protein, for pseudovirus productionDepositorInsertHCNV63gp2 (HCNV63gp2 human coronavirus NL63)
UseLentiviral and RetroviralTagsNAExpressionMammalianMutationc-terminal deletion of 14aaPromoterCMVAvailable sinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGL4.10/RSV_SF3B3-Xon_Luciferase
Plasmid#174659PurposePlasmid containing the SF3B3-Xon/Firefly luciferase cassette. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-xon by LMI070.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertXon cassette
UseLuciferaseExpressionMammalianMutationKozak ATG initiation sequence in middle exonPromoterRSVAvailable sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T3-GST-Tev
Plasmid#177142PurposeBacterial vector for expression of an N-terminal GST fusion of the Tev proteaseDepositorInsertTev
TagsGSTExpressionBacterialPromoterTacAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
eGFP-KIF5B
Plasmid#172203PurposeExpresses KIF5B tagged with eGFP in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertKIF5B (Kif5b Mouse)
TagseGFPExpressionMammalianPromoterCMVAvailable sinceNov. 8, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGEX-GST-His6-2_HBD
Plasmid#171870PurposeExpresses tandem repeats of HBD domain of RNase H1 for better DNA-RNA Hybrid recognition specificity in bacterial cellsDepositorInsertTandem repeats Hybrid binding domain of Rnase H1
TagsGST- and His10-tagExpressionBacterialMutationWTAvailable sinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.3_MERS_D12
Plasmid#170448PurposeExpress MERS WT spike protein with an 12aa deletion on the C-terminal tail. Used for MERS pseudotyped virus production.DepositorInsertMERS spike D12 (S MERS)
ExpressionMammalianMutationlast 12aa deletion in c-terminal tailPromoterCMVAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDONRp5-p2 GFP-NShroom3-iLID
Plasmid#170976PurposeN-terminal component of OptoShroom3 optogenetic tool. Binds to apical junctions. pDONRp5-p2 plasmid.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNShroom3 (Shroom3 Mouse)
UseGateway CloningTagseGFP and iLIDMutationFrom isoform 2, deleted amino acids 1389 - 1805,…Available sinceJuly 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-sgRNA-RFP
Plasmid#166005PurposeBacterial expression of dCas9 (aTc control) and sgRNA (arabinose control)DepositorTypeEmpty backboneUseCRISPRExpressionBacterialAvailable sinceApril 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
R619-M89-303: CMV51p> HCoV-HKU1 S-2P-T4f-3C-His8-Strep2x2
Plasmid#166014Purposemammalian expression of HCoV-HKU1 soluble spike trimer proteinDepositorInsertHCoV-HKU1 S (spike)
Tags3C-His8-Strep2x2ExpressionMammalianPromoterCMV51Available sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pQE30-HyPer7
Plasmid#141071PurposeBacterial expression of ultrasensitive hydrogen peroxide indicator HyPer7 for optical imagingDepositorInsertHyPer7
TagsHisx6ExpressionBacterialPromoterT5Available sinceMarch 1, 2021AvailabilityAcademic Institutions and Nonprofits only