166,041 results
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Plasmid#59386PurposeExpresses the lambda Red recombinase under control of the arabinose-inducible P(araB) promoter and I-SceI under control of the anhydrotetracycline-inducible P(tetA) promoter.DepositorTypeEmpty backboneUseGenome engineeringExpressionBacterialAvailable SinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only
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pET-Impact-T4gp43(exo-)
Plasmid#228202PurposeExpression in BL21(DE3) of 3' to 5' exonuclease-deficient T4 DNA polymerase D219ADepositorInsert43
TagsSce VMA intein-chitin binding domainExpressionBacterialMutationMutated aspartate 219 to alanine (D219A), elimina…PromoterT7Available SinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA HA DUSP6
Plasmid#236430Purposetransient overexpression of DUSP6 in mammalian cellsDepositorInsertDUSP6 (DUSP6 Human)
ExpressionMammalianAvailable SinceMay 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
p7SL-neo-TET
Plasmid#51285Purposeexpresses human 7SL RNA, using its own polIII enhancer, and tagged with the self splicing intron neo cassetteDepositorInsert7SL RNA (RN7SL2 Human)
TagsneoTET cassette with a tetrahymena self-splicing …ExpressionMammalianPromoter7SL upstream pollII enhancerAvailable SinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
MCP/PCP RNA Lantern
Plasmid#226095PurposeRNA trackingDepositorInsertNLS-HA-MS2-SmBiT-IRES-PP7-LgBiT-FLAG
ExpressionMammalianPromoterCMVAvailable SinceJan. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
HIP-flash (HOP-flash mutant)
Plasmid#83466PurposeNegative control for HOP-flash. 7xmut TEAD binding sites with minimal promoter plus luciferase reporter geneDepositorInsertMultimerized mutated TEAD binding sites in the promoter of CTGF with minimal promoter (CCN2 Human)
UseLuciferaseAvailable SinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-TO-dCas9-GFP
Plasmid#64106PurposeExpression of Sp dCas9-GFP in mammalian cellsDepositorInsertSp dCas9
UseCRISPR and LentiviralTagssfGFPExpressionMammalianPromoterCMV-TOAvailable SinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFBP-2_6.sensor
Plasmid#162800PurposeYeast expression of the RNA-based sensor for fructose-1,6-bisphosphate #2_6DepositorInsert2_6_RNA-device
ExpressionYeastAvailable SinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT7-VP1SA11
Plasmid#89162PurposeExpress Rotavirus SA11 VP1 in mammalian cellsDepositorInsertRotavirus SA11 VP1
ExpressionMammalianPromoterT7 promotorAvailable SinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
gTBEv4
Plasmid#220620Purposevector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv4) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoterDepositorInsertnSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
ExpressionMammalianMutationhUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…PromoterhU6, EF1aAvailable SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only