Skip to main content
Addgene
Showing: 5601 - 5650 of 6372 results

  3. JD180

    Plasmid
    #229486
    Purpose
    Adenoviral helper plasmid containing inducible E2A and L4-22k/33k genes
    Depositor
    Nerea Zabaleta
    Article
    Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3
    Insert
    E2A, L4-22k/33k, mCitrine
    Use
    AAV and Adenoviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tre (E2A, L4-22k/33k), ETR (mCitrine)
    Available since
    Dec. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    229486-plasmid-map-sequence-id-455846

    Enlarge

  5. MKC89_CCR5(UbC-tEPOR-2A-YFP)

    Plasmid
    #232404
    Purpose
    AAV production plasmid for UbC-tEPOR-2A-YFP vector from Fig. 2 that mediates HDR at CCR5 locus using CCR5-sg3 gRNA. YFP is followed by a BGH polyA. AAV genome is flanked by AAV2 ITRs.
    Depositor
    Kyle Cromer, Matthew Porteus
    Article
    Luna et al Nat Biomed Eng. 2024 Dec;8(12):1540-1552
    Insert
    Truncated Erythropoietin Receptor (EPOR Human)
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    232404-plasmid-map-sequence-id-462769

    Enlarge

  6. PX552

    Plasmid
    #60958
    Purpose
    pAAV-U6sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). AAV plasmid for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei.
    Depositor
    Feng Zhang
    Article
    Swiech et al Nat Biotechnol. 2014 Oct 19. doi: 10.103
    Inserts
    U6_(SpaI)_sgRNA
    Syn_EGFP-KASH
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Synapsin and U6
    Available since
    Nov. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    60958-plasmid-map-sequence-id-382829

    Enlarge

  16. pAT-mCherry2-G

    Plasmid
    #164169
    Purpose
    Expresses mCherry2 under the control of TetO promoter, basic vector for AAV, pAAV-CMV backbone, multiclonig site at C terminal, pAAV-TRE backbone
    Depositor
    Isei Tanida
    Article
    Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1
    Insert
    mCherry2
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetO
    Available since
    Feb. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    164169-plasmid-map-sequence-id-323964

    Enlarge

  18. pAT-mWasabi-G

    Plasmid
    #164170
    Purpose
    Expresses mWasabi under the control of TetO promoter, basic vector for AAV, pAAV-CMV backbone, multiclonig site at C terminal, pAAV-TRE backbone
    Depositor
    Isei Tanida
    Article
    Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1
    Insert
    mWasabi
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetO
    Available since
    Feb. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    164170-plasmid-map-sequence-id-323965

    Enlarge

  20. JD226

    Plasmid
    #229484
    Purpose
    Adenoviral helper plasmid containing E2A, VA RNA I and L4-22k genes
    Depositor
    Nerea Zabaleta
    Article
    Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3
    Insert
    E2A, VA RNA I, L4-22k
    Use
    AAV and Adenoviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV (E2A), Ef1a (L4-22k) and wt promoter (VA RNA …
    Available since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    229484-plasmid-map-sequence-id-455008

    Enlarge

  21. pVH009

    Plasmid
    #80399
    Purpose
    Control plasmid with no anti-scaffold feedback. Contains pBAD-CusR (response regulator) and pCusR-YFP-AAV.
    Depositor
    Richard Murray
    Article
    Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.
    Inserts
    3xFLAG-Venus YFP-AAV
    CusR (cusR )
    AraC (araC )
    Use
    Synthetic Biology
    Tags
    C-terminal LZx domain and N-terminal 3xFLAG tag, …
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    80399-plasmid-map-sequence-id-155900

    Enlarge

  23. psubCAG-WPRE-hVEGF-D-Strept

    Plasmid
    #119227
    Purpose
    Plasmid encodes Strep-tagged full-length (unprocessed) VEGF-D of human origin to be used for stimulation of lymphangiogenesis in mice in vivo.
    Depositor
    Kari Alitalo
    Insert
    VEGFD (VEGFD Human)
    Use
    AAV
    Tags
    Strept
    Expression
    Mutation
    Promoter
    CAG
    Available since
    Dec. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    119227-plasmid-map-sequence-id-231678

    Enlarge

  24. EP003: CAG::GEM7-HaloTag

    Plasmid
    #209303
    Purpose
    Expression plasmid for GEM7 (encapsulin from R. rubrum) with C-terminal HaloTag suitable for expression in mice via in utero electroporation
    Depositor
    Adam Cohen
    Insert
    GEM7
    Use
    AAV
    Tags
    HaloTag
    Expression
    Mutation
    Promoter
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    209303-plasmid-map-sequence-id-414428

    Enlarge

  26. p223-RV-dlx-dio-Tdtomato-T2A-SYPEGFP

    Plasmid
    #185699
    Purpose
    Retroviral vector expression cre-dependent Tdtomato-T2A-SynaptophysinEGFP driven by the mDlx promoter
    Depositor
    Lynette Lim
    Inserts
    Tdtomato,
    Synaptophysin-EGFP
    Use
    Cre/Lox and Retroviral
    Tags
    EGFP and T2A
    Expression
    Mammalian
    Mutation
    Promoter
    mDlx
    Available since
    July 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    185699-plasmid-map-sequence-id-366333

    Enlarge

  27. psubCMV-mVEGF-D-dNdC-WPRE

    Plasmid
    #119228
    Purpose
    Plasmid encodes proteolytically cleaved murine VEGF-D deltaN deltaC form, which has native signal peptide to be used for stimulation of lymphangiogenesis in mice in vivo.
    Depositor
    Kari Alitalo
    Insert
    Vegfd-dN-dC (Vegfd Mouse)
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    CMV
    Available since
    Dec. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    119228-plasmid-map-sequence-id-231686

    Enlarge

  28. p159-RV-syn-dio-mScarlet-T2A

    Plasmid
    #185700
    Purpose
    Retroviral vector expression cre-dependent membrane tagged mScarlet-T2A driven by the rat Synapsin promoter (with XhoI and PacI at 3'end of T2A to clone a gene of interest)
    Depositor
    Lynette Lim
    Insert
    mScarlet
    Use
    Retroviral
    Tags
    mScarlet palmitoylation with T2A on 3'-end
    Expression
    Mutation
    Promoter
    Synapsin
    Available since
    July 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    185700-plasmid-map-sequence-id-366334

    Enlarge

  29. JD150

    Plasmid
    #229485
    Purpose
    Adenoviral helper plasmid containing E2A, VA RNA I, E4orf6 and L4-22k genes
    Depositor
    Nerea Zabaleta
    Article
    Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3
    Insert
    E2A, VA RNA I, E4orf6, L4-22k/33k
    Use
    AAV and Adenoviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV (E2A, E4orf6), Ef1a (L4-22k) and wt promoter …
    Available since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    229485-plasmid-map-sequence-id-455002

    Enlarge

  30. JD171

    Plasmid
    #229483
    Purpose
    Adenoviral helper plasmid containing E2A, VA RNA I and L4-22k/33k genes
    Depositor
    Nerea Zabaleta
    Article
    Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3
    Insert
    E2A, VA RNA I, L4-22k/33k
    Use
    AAV and Adenoviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV (E2A), Ef1a (L4-22k/33k) and wt promoter (VA …
    Available since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    229483-plasmid-map-sequence-id-455009

    Enlarge

  37. JD179

    Plasmid
    #229496
    Purpose
    Adenoviral helper plasmid containing inducible E2A and L4-22k/33k genes and constitutive VA RNA I gene
    Depositor
    Nerea Zabaleta
    Article
    Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3
    Insert
    E2A, L4-22k/33k, VA RNA I
    Use
    AAV and Adenoviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tre (E2A, L4-22k/33k). Wt promoter (VA RNA I), ET…
    Available since
    Dec. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    229496-plasmid-map-sequence-id-455847

    Enlarge

  39. psubCMV-WPRE-IL3SP-mVEGF-D-dNdC

    Plasmid
    #119229
    Purpose
    Plasmid encodes proteolytically cleaved murine VEGF-D deltaN deltaC form, which has signal peptide from murine IL3 gene to be used for stimulation of lymphangiogenesis in mice in vivo.
    Depositor
    Kari Alitalo
    Insert
    IL3SP-Vegfd-dNdC (Vegfd Mouse)
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    CMV
    Available since
    March 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    119229-plasmid-map-sequence-id-239316

    Enlarge

  40. pDGM6

    Plasmid
    #110660
    Purpose
    Adeno-Associated Virus (AAV) serotype 6; contains the AAV6 cap genes, AAV2 rep genes, and adenovirus helper genes. Good for in vivo transduction of muscle, lung, liver and other tissues.
    Depositor
    David Russell
    Article
    Gregorevic et al Nat Med. 2004 Aug;10(8):828-34. doi: 10.
    Insert
    AAV6 cap genes, AAV2 rep genes, adenovirus helper genes
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    110660-plasmid-map-sequence-id-337382

    Enlarge

  42. 7M8

    Plasmid
    #64839
    Purpose
    This plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the 7M8 capsid protein.
    Depositor
    John Flannery, David Schaffer
    Article
    Dalkara et al Sci Transl Med. 2013 Jun 12;5(189):189ra
    Insert
    7M8 cap
    Use
    AAV
    Tags
    Expression
    Mutation
    7mer insertion in the AAV2 cap
    Promoter
    Available since
    Aug. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    64839-plasmid-map-sequence-id-342083

    Enlarge

  43. pTRE3G-Bi-gePSI-pGFAP-AcGFP

    Plasmid
    #133733
    Purpose
    Expresses both the gePSI-α-chain and the gePSI-β-chain under control of the inducible bi-directional TRE3G promoter. Expresses constitutively AcGFP under control of a GFAP promoter.
    Depositor
    Erin Schuman
    Article
    Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s
    Inserts
    gePSI-β-chain
    gePSI-α-chain
    AcGFP1
    Use
    AAV
    Tags
    murine ornithine decarboxylase - degron (ODC36)
    Expression
    Mammalian
    Mutation
    Promoter
    pGFAP and pTRE3G-Bi
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    133733-plasmid-map-sequence-id-265762

    Enlarge

  44. pTRE3G-Bi-gePSI-pCMV-AcGFP

    Plasmid
    #131008
    Purpose
    Expresses both the gePSI-α-chain and the gePSI-β-chain under control of the inducible bi-directional TRE3G promoter. Expresses constitutively AcGFP under control of a CMV promoter.
    Depositor
    Erin Schuman
    Article
    Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s
    Inserts
    gePSI-β-chain
    gePSI-α-chain
    AcGFP1
    Use
    AAV
    Tags
    murine ornithine decarboxylase - degron (ODC36)
    Expression
    Mammalian
    Mutation
    Promoter
    pCMV and pTRE3G-Bi
    Available since
    Dec. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    131008-plasmid-map-sequence-id-266807

    Enlarge

  49. pTRE3G-Bi-gePSI-pCamk2a-AcGFP

    Plasmid
    #133732
    Purpose
    Expresses both the gePSI-α-chain and the gePSI-β-chain under control of the inducible bi-directional TRE3G promoter. Expresses constitutively AcGFP under control of a Camk2a promoter.
    Depositor
    Erin Schuman
    Article
    Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s
    Inserts
    gePSI-β-chain
    gePSI-α-chain
    AcGFP1
    Use
    AAV
    Tags
    murine ornithine decarboxylase - degron (ODC36)
    Expression
    Mammalian
    Mutation
    Promoter
    pCamk2a and pTRE3G-Bi
    Available since
    Dec. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    133732-plasmid-map-sequence-id-266808

    Enlarge

Showing: 5601 - 5650 of 6372 results