We narrowed to 9,182 results for: Pol;

1. pET21a - EtfB

   Plasmid

   #89617

   Purpose

   Expresses recombinant 6HIS tagged T. cruzi electron transfer flavoprotein B in E. coli upon IPTG induction

   Depositor

   Judy Lieberman

   Article

   Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

   Insert

   electron-transfer-flavoprotein, alpha polypeptide
   

   Tags

   6HIS and T7 tag (gene 10 leader)

   Expression

   Bacterial

   Available Since

   May 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMpGWB434

   Plasmid

   #68699

   Purpose

   Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Tags

   Glucocorticoid receptor (GR)

   Expression

   Plant

   Promoter

   MpHSP17.8A1 promoter

   Available Since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMpGWB433

   Plasmid

   #68698

   Purpose

   Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Tags

   Citrine

   Expression

   Plant

   Promoter

   MpHSP17.8A1 promoter

   Available Since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMpGWB410

   Plasmid

   #68675

   Purpose

   Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Tags

   3xFLAG

   Expression

   Plant

   Promoter

   MpEF1alpha promoter

   Available Since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMpGWB413

   Plasmid

   #68678

   Purpose

   Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Tags

   Glucocorticoid receptor (GR)

   Expression

   Plant

   Promoter

   MpEF1alpha promoter

   Available Since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMpGWB233

   Plasmid

   #68624

   Purpose

   Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Tags

   Citrine

   Expression

   Plant

   Promoter

   MpHSP17.8A1 promoter

   Available Since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMpGWB210

   Plasmid

   #68601

   Purpose

   Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Tags

   3xFLAG

   Expression

   Plant

   Promoter

   MpEF1alpha promoter

   Available Since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCellFree_G03 TCEB3B

   Plasmid

   #67117

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   TCEB3B (ELOA2 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pPB_TRE3G_scFV-sfGFP-Prdm9CD

   Plasmid

   #235577

   Purpose

   Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Prdm9 fused with scFV to programme H3K4me3

   Depositor

   Jamie Hackett

   Article

   Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

   Insert

   Prdm9 (Prdm9 Mouse)
   

   Use

   CRISPR

   Available Since

   April 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPB_TRE3G_scFV-sfGFP-Prdm9CD-CatMut

    Plasmid

    #235586

    Purpose

    Dox-inducible expression of control catalytic-mutant Prdm9 CD fused with scFV

    Depositor

    Jamie Hackett

    Article

    Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

    Insert

    Prdm9 (Prdm9 Mouse)
    

    Use

    CRISPR

    Mutation

    G282A

    Available Since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPB_TRE3G_scFV-sfGFP-Ring1bCD

    Plasmid

    #235574

    Purpose

    Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Ring1b fused with scFV to programme H2AK119ub.

    Depositor

    Jamie Hackett

    Article

    Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

    Insert

    Ring1b (Rnf2 Mouse)
    

    Use

    CRISPR

    Available Since

    April 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMpGE011

    Plasmid

    #71537

    Purpose

    For CRISPR/Cas9 genome editing in Marchantia polymorpha

    Depositor

    Takayuki Kohchi

    Article

    Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pVDL9.3

    Plasmid

    #168299

    Purpose

    Secretion of polypeptides (nanobodies) in E. coli fused to C-terminal secretion signal of HlyA

    Depositor

    Víctor de Lorenzo

    Article

    Tzschaschel et al Nat Biotechnol. 1996 Jun;14(6):765-9. do

    Insert

    Segment of the hly operon, spanning the C-terminal part of hlyA, hlyB, and hlyD
    

    Expression

    Bacterial

    Promoter

    LacI-Plac

    Available Since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPB_TRE3G_scFV-sfGFP-Setd2CD

    Plasmid

    #235579

    Purpose

    Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Setd2 fused with scFV to programme H3K36me3

    Depositor

    Jamie Hackett

    Article

    Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

    Insert

    Setd2 (Setd2 Mouse)
    

    Use

    CRISPR

    Available Since

    April 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pACEBac1-PARP2-Flag-His-WT

    Plasmid

    #111574

    Purpose

    Expresses full length PARP2 in Sf9 (insect) cells

    Depositor

    Thomas Muir

    Article

    Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

    Insert

    PARP2 (PARP2 Human)
    

    Tags

    C-terminal 6xHis and C-terminal Flag

    Expression

    Insect

    Promoter

    polyhedrin promoter

    Available Since

    July 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMpGE_En03

    Plasmid

    #71535

    Purpose

    Used to construct a gRNA expression cassette for CRISPR/Cas9 genome editing in Marchantia polymorpha

    Depositor

    Takayuki Kohchi, Sumihare Noji

    Article

    Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGTRm

    Plasmid

    #127197

    Purpose

    pGTRm is the template plasmid for PCR & golden gate assembly driven generation of polycistronic tRNA-gRNA sequences

    Depositor

    Jordan Green

    Article

    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

    Insert

    tRNA-gRNA PCR template
    

    Use

    Template plasmid for generation of golden gate pc…

    Promoter

    None

    Available Since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET21 MBPx-SpyCatcher

    Plasmid

    #72327

    Purpose

    Bacterial expression of tandem high affinity maltose binding protein domains linked to SpyCatcher, for solid-phase polyprotein synthesis.

    Depositor

    Mark Howarth

    Article

    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

    Insert

    pET21 MBPx-SpyCatcher
    

    Tags

    N-terminal His6 tag

    Expression

    Bacterial

    Mutation

    MBP contains A312V, I317V mutations and 172, 173,…

    Available Since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFastBac-His-MBP-SCov2-nsp12

    Plasmid

    #154759

    Purpose

    Expression of SARS-CoV-2 nsp12

    Depositor

    Patrick Cramer

    Article

    Hillen et al Nature. 2020 May 21. pii: 10.1038/s41586

    Insert

    SARS-CoV-2 nsp12 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Insect

    Promoter

    Polyhedron

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCRISPRa_VPR_yl

    Plasmid

    #107677

    Purpose

    CRISPR-dCas9-VPR vector for Yarrowia lipolytica, expressing dCas9-VPR and AvrII site for sgRNA insertion

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al Biotechnol J. 2018 May 5:e1700584. doi:

    Inserts

    Codon optimized dCas9-VPR
    sgRNA expression cassette
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Promoter

    SCR1'-tRNA and UAS1B8-TEF(136)

    Available Since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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