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PRK5-FLAG-USP13/5-6

Plasmid

#61748

Purpose

express N-terminally FLAG tagged human chimera USP13/5-6

Depositor

Yihong Ye

Article

Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

Insert

USP13/5-6 chimera (USP5, USP13 Human)

Tags

FLAG

Expression

Mammalian

Mutation

USP13 (1-721aa) fused to USP5 (717-859aa); D23Y i…

Promoter

CMV

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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PRK5-FLAG-USP13/5-5

Plasmid

#61747

Purpose

express N-terminally FLAG tagged human chimera USP13/5-5

Depositor

Yihong Ye

Article

Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

Insert

USP13/5-5 chimera (USP5, USP13 Human)

Tags

FLAG

Expression

Mammalian

Mutation

USP5 (1-781aa) fused to USP13 (786-864aa); G2667A…

Promoter

CMV

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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PRK5-FLAG-USP13/5-3

Plasmid

#61745

Purpose

express N-terminally FLAG tagged human chimera USP13/5-3

Depositor

Yihong Ye

Article

Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

Insert

USP13/5-3 chimera (USP5, USP13 Human)

Tags

FLAG

Expression

Mammalian

Mutation

USP5 (1-628aa) fused to USP13 (653-864aa)

Promoter

CMV

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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PRK5-FLAG-USP13/5-2

Plasmid

#61744

Purpose

express N-terminally FLAG tagged human chimera USP13/5-2

Depositor

Yihong Ye

Article

Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

Insert

USP13/5-2 chimera (USP5, USP13 USP13/5-2 chimera, Human)

Tags

FLAG

Expression

Mammalian

Mutation

USP5 (1-409aa) fused to USP13 (421-864aa)

Promoter

CMV

Available Since

Feb. 4, 2015

Availability

Academic Institutions and Nonprofits only

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PRK5-FLAG-USP13/5-1

Plasmid

#61743

Purpose

express N-terminally FLAG tagged human chimera USP13/5-1

Depositor

Yihong Ye

Article

Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

Insert

USP13/5-1 chimera (USP5, USP13 Human)

Tags

FLAG

Expression

Mammalian

Mutation

USP5 (1-185aa) fused to USP13 (197-864aa)

Promoter

CMV

Available Since

Feb. 4, 2015

Availability

Academic Institutions and Nonprofits only

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FLAG-WT-short-UBASH3A

Plasmid

#192090

Purpose

Mammalian expression of human wild-type short-form UBASH3A with FLAG tag

Depositor

Patrick Concannon

Insert

UBASH3A (UBASH3A Human)

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV_FLAG-NFL(K211TAG)

Plasmid

#182660

Purpose

Expresses mouse neurofilament light chain with a TAG codon at the position K211 and an N-terminal FLAG tag (DYKDDDDK) in mammalian cells.

Depositor

Ivana Nikić-Spiegel

Article

Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

Insert

mouse neurofilament light chain with a K211TAG mutation (Nefl Mouse)

Tags

FLAG tag (DYKDDDDK)

Expression

Mammalian

Mutation

K211TAG

Promoter

CMV

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-VenusFlag-hTICAM1

Plasmid

#197631

Purpose

Human TRIF protein expression in mammalian cells

Depositor

Haian Fu

Insert

TICAM1 (TICAM1 Human)

Tags

Venus-Flag

Expression

Mammalian

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-HA-FbxO10-pcDNA3.1-

Plasmid

#52508

Purpose

expresses human FbxO10 in mammalian cells with FLAG-HA tag at N-terminus

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

FbxO10 (FBXO10 Human)

Tags

FLAG-HA

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-C2_3X Flag

Plasmid

#194161

Purpose

Expresses eGFP fluorescent protein with a 3X flag tag

Depositor

Stefan Stamm

Article

Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

Insert

GFP with 3X Flag

Tags

3x Flag

Expression

Mammalian

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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MigR1-Flag-TRIB2

Plasmid

#131154

Purpose

Retroviral expression of flag-tagged mouse TRIB2

Depositor

Karen Keeshan, Warren Pear

Article

Keeshan et al Cancer Cell. 2006 Nov;10(5):401-11. doi:

Insert

Trib2 (Trib2 Mouse)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pRK Smad3LC Flag

Plasmid

#14829

Depositor

Rik Derynck

Article

Zhang et al Curr Biol. 1997 Apr 1. 7(4):270-6.

Insert

Smad3 LC (SMAD3 Human)

Tags

Flag

Expression

Mammalian

Mutation

amino acids 146-425

Available Since

May 15, 2007

Availability

Academic Institutions and Nonprofits only
pKK-TEV-FLAG

Plasmid

#105783

Purpose

Expression of your protein of interest in fusion with FLAG at the C-terminus. The tag is cleavable by TEV protease.

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

TEV-FLAG

Expression

Mammalian

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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Flag-TRIM24 shRescue

Plasmid

#28139

Depositor

Michelle Barton

Article

Allton et al Proc Natl Acad Sci U S A. 2009 Jul 14. 1

Insert

TRIM24 (TRIM24 Human)

Tags

FLAGX2

Expression

Mammalian

Mutation

A489V; aa 308 and 309 mutated for shRNA rescue

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
Flag-MCPIP1(1-453)

Plasmid

#90106

Purpose

Mammalian expression of Flag-MCPIP1(1-453)

Depositor

Mingui Fu

Article

Liang et al J Exp Med. 2010 Dec 20;207(13):2959-73.

Insert

MCPIP1 (ZC3H12A Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pCR3-FLAG-Gephyrin G

Plasmid

#68817

Purpose

Geph G domain expression in mamalian cells

Depositor

Shiva Tyagarajan

Article

Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

Insert

Gephyrin (Gphn Rat)

Tags

3xFLAG

Expression

Mammalian

Mutation

NIL

Promoter

CMV

Available Since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-flag-UTR

Plasmid

#183234

Purpose

Mammalian expression empty backbone

Depositor

Jorge Ferrer

Article

Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

Type

Empty backbone

Tags

FLAG tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Lazarus Gata 3 flag

Plasmid

#80135

Purpose

retroviral expression of Gata3-Flag

Depositor

Ellen Rothenberg

Insert

GATA binding protein 3 (Gata3 Mouse)

Use

Retroviral

Tags

FLAG and IRES-EGFP

Expression

Mammalian

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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FLAG-EAF pBacPAK8

Plasmid

#15323

Depositor

Joan Conaway, Ronald Conaway

Article

Kong et al Proc Natl Acad Sci U S A. 2005 Jul 19. 1

Insert

ELL Associated Factor (EAF1 Human)

Use

Baculovirus construction

Tags

6x His and FLAG

Promoter

polyhedrin

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CARMA2cl-FLAG

Plasmid

#97077

Purpose

Expresses CARMA2cl/CARD14 isoform 2 in mammalian cells

Depositor

Pasquale Vito

Article

Scudiero et al J Cell Physiol. 2011 Dec;226(12):3121-31

Insert

CARD14 isoform 2 (CARD14 Human)

Tags

FLAG

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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