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We narrowed to 2,454 results for: puc19

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Showing: 2241 - 2260 of 2454 results

  1. pMT1461

    Plasmid
    #228319
    Purpose
    Doxycycline-regulatable ALX-0081 expression
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi
    Insert
    MFalpha(2xAdv)-ALX-0081-3A-FLAG-His
    Use
    Affinity Reagent/ Antibody
    Tags
    AAA linker followed by FLAG-His6 and MF_ secretio…
    Expression
    Yeast
    Promoter
    Doxycycline-regulatable synthetic promoter (KpTc7…
    Available Since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228319-plasmid-map-sequence-id-457683

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  2. pMT1026

    Plasmid
    #228299
    Purpose
    Constitutive expression of PhlTA mutant with weak promoter and terminator
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi
    Insert
    phlTA mutant
    Use
    Synthetic Biology
    Tags
    Three copies of VP16 (VP48)-Nuclear localization …
    Expression
    Yeast
    Mutation
    E41G
    Promoter
    KpYPT1 promoter
    Available Since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228299-plasmid-map-sequence-id-457081

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  3. pMT478

    Plasmid
    #228286
    Purpose
    Constitutive mutant rPhlTA expression with strong promoter
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi
    Insert
    rphlTA mutant
    Use
    Synthetic Biology
    Tags
    Three copies of VP16 (VP48)-Nuclear localization …
    Expression
    Yeast
    Mutation
    P5S, S6P, K86T, F109L, Q117R, E143K
    Promoter
    KpGAPDH promoter
    Available Since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228286-plasmid-map-sequence-id-457102

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  5. PE-pegRNA(BG)-nsgRNA(BG)

    Plasmid
    #232727
    Purpose
    A dual-targeting vector that contains PE3 system guide RNAs against BFP (pegRNA(BG) and nsgRNA(BG)) with restriction enzyme sites for insertion of target site guide RNAS (pegRNAs(TS) and nsgRNA(TS)).
    Depositor
    David Brafman
    Article
    Frisch et al Mol Ther Nucleic Acids. 2023 Jul 17;33:4
    Insert
    pegRNA(BG), nsgRNA(BG), pegRNAs(TS), nsgRNA(TS)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232727-plasmid-map-sequence-id-462122

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  6. qTAG-C-Solo-mScarlet-MAPRE1

    Plasmid
    #227323
    Purpose
    Donor template for mScarlet insertion into the C-terminus of the MAPRE1 locus. For growing microtubule tip visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MAPRE1 (Addgene #207793)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    MAPRE1 Homology Arms flanking a mScarlet Tag (MAPRE1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    227323-plasmid-map-sequence-id-451725

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  7. qTAG-KO-Puro-TP53

    Plasmid
    #227319
    Purpose
    Donor template for 2A-Puro insertion into the N-terminus of the TP53 locus. For selectable p53 knock-out. To be co-transfected with sgRNA plasmid px330-TP53 (Addgene #227318)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    TP53 Homology Arms flanking a 2A-Puro Cassette (TP53 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    227319-plasmid-map-sequence-id-451734

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  8. qTAG-C-Solo-mStayGold-EZR

    Plasmid
    #227294
    Purpose
    Donor template for mStayGold insertion into the C-terminus of the EZR locus. For membrane visualization. To be co-transfected with sgRNA plasmid px330-EZR (Addgene #227293)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    EZR Homology Arms flanking a mStayGold Tag (EZR Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    227294-plasmid-map-sequence-id-451303

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  11. qTAG-C-Solo-mStayGold-H3C2

    Plasmid
    #227334
    Purpose
    Donor template for mStayGold insertion into the C-terminus of the H3C2 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 (Addgene #207780)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H3C2 Homology Arms flanking a mStayGold Tag (H3C2 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    227334-plasmid-map-sequence-id-453638

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  12. qTAG-C-Solo-miRFP670nano3-H3C2

    Plasmid
    #227335
    Purpose
    Donor template for miRFP670nano3 insertion into the C-terminus of the H3C2 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 (Addgene #207780)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H3C2 Homology Arms flanking a miRFP670nano3 Tag (H3C2 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227335-plasmid-map-sequence-id-452142

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  13. qTAG-N-Solo-mStayGold-MAP4

    Plasmid
    #227296
    Purpose
    Donor template for mStayGold insertion into the N-terminus of the MAP4 locus. For microtubule visualization. To be co-transfected with sgRNAplasmid px330-PITCh-MAP4 (Addgene #227295)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    MAP4 Homology Arms flanking a mStayGold Tag (MAP4 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227296-plasmid-map-sequence-id-452068

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  14. qTAG-C-Solo-mStayGold-CEP192

    Plasmid
    #227289
    Purpose
    Donor template for mStayGold insertion into the C-terminus of the CEP192 locus. For pericentriolar material visualization. To be co-transfected with sgRNA plasmid px330-PITCh-CEP192 (Addgene #227288)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    CEP192 Homology Arms flanking a mStayGold Tag (CEP192 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227289-plasmid-map-sequence-id-452061

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  15. qTAG-C-Solo-mStayGold-ARL13B

    Plasmid
    #227277
    Purpose
    Donor template for mStayGold insertion into the C-terminus of the ARL13B locus. For cilia visualization. To be co-transfected with sgRNA plasmid pX330-PITCh-ARL13B (Addgene #227276)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    ARL13B Homology Arms flanking a mStayGold Tag (ARL13B Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227277-plasmid-map-sequence-id-452043

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  16. qTAG-C-mStayGold-EFS-Puro-CEP192

    Plasmid
    #227290
    Purpose
    Donor template for mStayGold-EFS-Puro insertion into the C-term of CEP192 locus. For pericentriolar material visualization. To be co-transfected with sgRNA plasmid px330-PITCh-CEP192 (Addgene #227288)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    CEP192 Homology Arms flanking a mStayGold-EFS-Puro Cassette (CEP192 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    227290-plasmid-map-sequence-id-451300

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  17. pILGFPEasy9R

    Plasmid
    #218291
    Purpose
    Plasmid-free in-situ promoter cloning and characterisation system in S. cerevisiae, allows the characterisation of a bidirectional promoter using yEGFP and E2-Crimson as reporters.
    Depositor
    Claudia Vickers
    Article
    McDonnell et al bioRxiv 2024.03.26.586389
    Insert
    pURA3>KlURA3>tKlURA3-tPGK1KanMX>tAgTEF-pMAL32>MazF(RPL28i)-E2Crimson>tPDC1-pDDI2>ISceI>tSynth3>ura3(4,191)
    Expression
    Yeast
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218291-plasmid-map-sequence-id-433736

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  18. pToxAmp21R

    Plasmid
    #218287
    Purpose
    A complete plasmid for ToxAmp (Toxin-antitoxin-driven gene amplification) for the expression of AeBlue
    Depositor
    Claudia Vickers
    Article
    Evans et al bioRxiv 2024.01.30.578080
    Insert
    HO(-955, -789)-LoxP>pKlLEU2>KlLEU2>PCRT1>RelB>tPDC1-pTDH3>AeBlue>tSYNth7-ARS712-HO(-731, -264)
    Expression
    Yeast
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218287-plasmid-map-sequence-id-433689

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  19. pToxAmp12

    Plasmid
    #218286
    Purpose
    Multi-copy integration of heterologous genes (AeBlue and RelB) through co-transformation with ToxAmp (toxin-antitoxin-driven gene amplification) modules
    Depositor
    Claudia Vickers
    Article
    Evans et al bioRxiv 2024.01.30.578080
    Insert
    HO(-253, -1)-pCRT1>RelB>tPDC1-pTDH3>AeBlue>tsynth7-ARS712-HO(-731, -264)
    Expression
    Yeast
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218286-plasmid-map-sequence-id-433688

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  20. pToxAmp1

    Plasmid
    #218285
    Purpose
    Amplifying the gene copy number of heterologous genes (yEGFP and RelB) through ToxAmp (toxin-antitoxin-driven gene amplification) mechanism
    Depositor
    Claudia Vickers
    Article
    Evans et al bioRxiv 2024.01.30.578080
    Insert
    HO(-253, -1)-pRPL8B>RelB>tPDC1-pTEF1>yEGFP>tURA3-ARS712-HO(-731, -264)
    Expression
    Yeast
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218285-plasmid-map-sequence-id-433687

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Showing: 2241 - 2260 of 2454 results