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pLenti_CMVTRE3G_LYSET-Isoform2_untagged-Puro

Plasmid

#192002

Purpose

Lentiviral vector to generate LYSET(TMEM251)-isoform2 stable expressing cell line under CMVTRE3G promoter

Depositor

Jan Carette

Article

Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

Insert

LYSET-Isoform2 (LYSET Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMVTRE3G

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti_CMVTRE3G_LYSET-Isoform1_I142V-Puro

Plasmid

#192004

Purpose

Lentiviral vector to generate LYSET(TMEM251)-isoform1 I142V mutant stable expressing cell line under CMVTRE3G promoter

Depositor

Jan Carette

Article

Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

Insert

LYSET-Isoform1 I142V (LYSET Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMVTRE3G

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti_CMVTRE3G_LYSET-Isoform1_R45W-Puro

Plasmid

#192003

Purpose

Lentiviral vector to generate LYSET(TMEM251)-isoform1 R45W mutant stable expressing cell line under CMVTRE3G promoter

Depositor

Jan Carette

Article

Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

Insert

LYSET-Isoform1 R45W (LYSET Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMVTRE3G

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti_CMVTRE3G_LYSET-Isoform2-R45W-Flag

Plasmid

#192005

Purpose

Lentiviral vector to generate flag-tagged LYSET(TMEM251)-isoform2 R45W mutant stable expressing cell line under CMVTRE3G promoter

Depositor

Jan Carette

Article

Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

Insert

LYSET-Isoform2-R45W (LYSET Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMVTRE3G

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-YTH-mCherry-Cry2

Plasmid

#177126

Purpose

Express YTH domain fragment of YTHDC1 with mCherry and CRY2 tag for Optodroplet Assay

Depositor

Wenbo Li

Article

Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

Insert

YTHDC1 (YTH domain) (YTHDC1 Human)

Tags

mCherry-CRY2

Expression

Mammalian

Mutation

YTH domain only

Promoter

SFFV

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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Pet22b-mEGFP-YAP-C-IDR

Plasmid

#166441

Purpose

Bacterial expression of 6x His tagged mEGFP-YAP1-C-IDR fusion protein

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, YAP-C-IDR(266-504) (YAP1 Human)

Expression

Bacterial

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pmiRA1-MYB

Plasmid

#141120

Purpose

Lentiviral overexpression of MYB in mammalian cells.

Depositor

Jianjun Chen

Article

Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

Insert

MYB (MYB Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 14, 2020

Availability

Academic Institutions and Nonprofits only

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gCH130 (crCD55-4_crB2M-1_crKIT-2_crCD81-1)

Plasmid

#217340

Purpose

crRNA array targeting CD81, B2M, KIT, CD55

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Inserts

crCD55-4 gRNA: actggtattgcggagccacgagg (CD55 Human)
crB2M-1 gRNA: atataagtggaggcgtcgcgctg (B2M Human)
crKIT-2 gRNA: tctgcgttctgctcctactgctt (KIT Human)
crCD81-1 gRNA: ggcgcgacccccaggaaggtctc (CD81 Human)

Use

CRISPR and Lentiviral

Promoter

hU6

Available Since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-Ef1a-mClover-W377A/W428A YTHDC1-T2A-BSD-siRNA-Resistant

Plasmid

#177130

Purpose

Generate lentivirus to introduce mClover tagged m6A binding null mutant W377A/W428A YTHDC1

Depositor

Wenbo Li

Article

Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

Insert

YTHDC1 (YTHDC1 Human)

Use

Lentiviral

Tags

mClover3

Mutation

W428A/W377A (m6A binding defects), and siRNA resi…

Promoter

Ef1a

Available Since

July 7, 2022

Availability

Academic Institutions and Nonprofits only

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PCDH-METTL14-R298P

Plasmid

#141113

Purpose

Overexpression of METTL14 mutant in mammalian cells.

Depositor

Jianjun Chen

Article

Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

Insert

HMETTL14 (METTL14 Human)

Expression

Mammalian

Mutation

Substitution - Missense, position 298, R➞P

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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