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Showing: 2261 - 2280 of 11761 results

  1. G protein alpha-q-CFP

    Plasmid
    #66081
    Purpose
    This G protein alpha-q construct contains internal insertions of CFP and the EE epitope
    Depositor
    Catherine Berlot
    Article
    Hughes et al J Biol Chem. 2001 Feb 9;276(6):4227-35.
    Insert
    G protein alpha-q-EE-CFP (Gnaq Mouse)
    Tags
    CFP was inserted internally into the alpha-q sequ…
    Expression
    Mammalian
    Mutation
    BamHI and SacI sites in alpha-q were removed with…
    Promoter
    CMV
    Available Since
    July 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    66081-plasmid-map-sequence-id-118921

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  11. pCBh-T7-SpG-P2A-mTagBFP2(RAS3583)

    Plasmid
    #223110
    Purpose
    pCBh Human expression plasmid for SpG with with C-term BPNLS-P2A-mTagBFP2
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpG-P2A-mTagBFP2
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-mTagBFP2
    Expression
    Mammalian
    Mutation
    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223110-plasmid-map-sequence-id-447633

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  14. B9-COMP-blac-flag-his

    Plasmid
    #111020
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    6-cysteine protein (B9) (PF3D7_0317100 )
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available Since
    Oct. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    111020-plasmid-map-sequence-id-224595

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  17. GRP94-COMP-blac-flag-his

    Plasmid
    #111008
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    endoplasmin, putative (GRP94) (PFL1070c Synthetic)
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available Since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    111008-plasmid-map-sequence-id-224562

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  18. pαH-S-GSAS-OMICRON.BA.2-RBD

    Plasmid
    #184830
    Purpose
    Mammalian cell expression of RBD of SARS-CoV-2 spike protein of variant OMICRON.BA.2 with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987
    Depositor
    Priyamvada Acharya
    Article
    Stalls et al Cell Rep. 2022 Jun 28;39(13):111009. doi
    Insert
    S-GSAS-OMICRON.BA2-RBD (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    6X His tag
    Expression
    Mammalian
    Mutation
    SARS-CoV-2 Spike RBD protein of OMICRON.BA.2 vari…
    Available Since
    June 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    184830-plasmid-map-sequence-id-363832

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  19. pαH-S-GSAS-OMICRON.BA.1-RBD

    Plasmid
    #184831
    Purpose
    Mammalian cell expression of RBD of SARS-CoV-2 spike protein of variant OMICRON.BA.1 with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987
    Depositor
    Priyamvada Acharya
    Article
    Stalls et al Cell Rep. 2022 Jun 28;39(13):111009. doi
    Insert
    S-GSAS-OMICRON.BA.1 (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    6X His tag
    Expression
    Mammalian
    Mutation
    SARS-CoV-2 Spike RBD protein of OMICRON.BA.1 vari…
    Available Since
    June 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    184831-plasmid-map-sequence-id-363833

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  20. AAP-COMP-blac-flag-his

    Plasmid
    #111001
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    aminopeptidase P (APP) (PF14_0517 Synthetic)
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available Since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    111001-plasmid-map-sequence-id-224546

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Showing: 2261 - 2280 of 11761 results