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pJL30

Plasmid

#174064

Purpose

Encodes for 43-8 ZF fused to Cry2. Used for optogenetic recruitment of TF. Used with pJL32.

Depositor

Albert Keung

Article

Lee et al Cell Syst. 2021 Aug 24. pii: S2405-4712(

Insert

pGal1-TetO-3xNLS-43-8ZF-Cry2-tADH1

Tags

FLAG

Expression

Yeast

Promoter

pGal1

Available Since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CTAG_linker-Lti6b

Plasmid

#136099

Purpose

Plasma Membrane targeting protein for C-term fusion with CDS12 without linker.

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CTAG_AlaSerGly10xAla_Lti6b

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CTAG_linker-N7

Plasmid

#136102

Purpose

Nuclear targeting peptide for C-term fusion with CDS12 without linker.

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CTAG_AlaSer4xAla_N7

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pJL15

Plasmid

#174069

Purpose

Expresses 43-8 zinc finger array fused to CRY2 in S. cerevisiae. Part of optogenetic system.

Depositor

Albert Keung

Article

Lee et al Cell Syst. 2021 Aug 24. pii: S2405-4712(

Insert

pGAL-tetO-3xNLS-43-8WT-GS-Cry2-Tadh1

Tags

FLAG

Expression

Yeast

Promoter

pGal1

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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UBC1cer40GEM

Plasmid

#172699

Purpose

Ubiquitous expression of 60meric GEM monomer

Depositor

Krishna Niyogi

Article

Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

Insert

monomer of 60mer GEM

Use

Unspecified

Tags

GFP

Expression

Plant

Promoter

UBC1

Available Since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

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pZA17

Plasmid

#158495

Purpose

In planta gene expression of Golden gate compatible A. thaliana ZAR1. Km plant selection marker.

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1 (ZAR1 Mustard Weed)

Expression

Plant

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE477

Plasmid

#153268

Purpose

M3 shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE478

Plasmid

#153269

Purpose

M4 shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE501

Plasmid

#153274

Purpose

M7 shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE499

Plasmid

#153272

Purpose

M6 shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE498

Plasmid

#153271

Purpose

M5 shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR207-OEP7(1–50)

Plasmid

#100601

Purpose

Gateway entry vector for AtOEP7(1–50)

Depositor

Yutaka Kodama

Article

Tanaka et al J Plant Res. 2017 Jun 20. doi: 10.1007/s

Insert

OEP7(1–50) (OEP7 Mustard Weed)

Use

Gateway entry vector

Available Since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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MTK4a_024

Plasmid

#123836

Purpose

Encodes Abscisic Acid Insensitive as a Type 4a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

ABI

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pN_35S/CTP-fluoA

Plasmid

#117990

Purpose

Chloroplast-targeted expression of CURT_fluoA controlled by the CaMV 35S promoter

Depositor

Mathias Pribil

Article

Behrendorff et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

CTP-CURT_fluoA

Use

Synthetic Biology; Plant/bacteria binary vector

Expression

Plant

Promoter

Cauliflower mosaic virus 35S

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pN_35S/CTP-fluoB

Plasmid

#117991

Purpose

Chloroplast-targeted expression of CURT_fluoB controlled by the CaMV 35S promoter

Depositor

Mathias Pribil

Article

Behrendorff et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

CTP-CURT_fluoB

Use

Synthetic Biology; Plant/bacteria binary vector

Expression

Plant

Promoter

Cauliflower mosaic virus 35S

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pN_35S/CTP-fluoE

Plasmid

#117992

Purpose

Chloroplast-targeted expression of CURT_fluoE controlled by the CaMV 35S promoter

Depositor

Mathias Pribil

Article

Behrendorff et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

CTP-CURT_fluoE

Use

Synthetic Biology; Plant/bacteria binary vector

Expression

Plant

Promoter

Cauliflower mosaic virus 35S

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pN_35S/CURT_flouB

Plasmid

#117995

Purpose

Expression of CURT_flouB controlled by the CaMV 35S promoter

Depositor

Mathias Pribil

Article

Behrendorff et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

CURT_flouB

Use

Yeast/bacteria binary vector

Expression

Plant

Promoter

Cauliflower mosaic virus 35S

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pUG-UBC23

Plasmid

#104688

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

AtUBC23 (UBC23 Mustard Weed)

Use

Synthetic Biology

Mutation

BpiI/BsaI sites removed by point-mutation

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pRPL28 CaURA3 (pNTI592)

Plasmid

#115431

Purpose

URA3-marked RPS28

Depositor

Nicholas Ingolia

Article

McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

Inserts

RPL28 (RPL28 Budding Yeast)
URA3

Expression

Yeast

Mutation

KpnI site in intron

Promoter

Ashbya gossypii and endogenous

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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Osa-pCAMBIA-1301-STTM535

Plasmid

#84227

Purpose

Disturb the expression of miR535 in rice

Depositor

Guiliang Tang

Article

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR535

Expression

Bacterial, Plant, and Yeast…

Promoter

2x35S

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only