We narrowed to 22,070 results for: ESC

1. pREF-GFP kan

   Plasmid

   #122061

   Purpose

   reporter plasmid for termination and terminator override to express GFP. Marked with URA3

   Depositor

   Daniel Reines

   Article

   Reines et al Methods. 2019 Jan 4. pii: S1046-2023(18)

   Inserts

   intergenic terminator from yeast IMD2
   green fluorescent protein
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1 and GAL1 comes in pYES2

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. Nano-lantern(Ca2+)_150/pcDNA3

   Plasmid

   #51984

   Purpose

   mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=150nM

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern_CaM-2G
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S257G in Rluc8

   Promoter

   Available since

   April 15, 2014

   Availability

   Academic Institutions and Nonprofits only

3. Nano-lantern(Ca2+)_11/pcDNA3

   Plasmid

   #51987

   Purpose

   mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=11nM

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern_CaM-4GS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S257G in Rluc8

   Promoter

   Available since

   March 25, 2014

   Availability

   Academic Institutions and Nonprofits only

4. LbCas12a

   Plasmid

   #188493

   Purpose

   Expresses FLAG tagged LbCas12a

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   Cas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMyosin-miRFP670nano

   Plasmid

   #127431

   Purpose

   LC-Myosin labeling with near-infrared fluorescent protein miRFP670nano

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

   Insert

   miRFP670nano-LC-Myosin
   

   Use

   Tags

   miRFP670nano

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGBW-m4046871

   Plasmid

   #145724

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 nsp10

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp10 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 27, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pGBW-m4046731

   Plasmid

   #145633

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 nsp2

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp2 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 28, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pSEM229 - [Pmlc-1 | mNeonGreen | cbr-tbb-2 UTR]

   Plasmid

   #159896

   Purpose

   Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

   Insert

   Pmlc-1 | mNeonGreen | cbr-tbb-2 UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Oct. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. DGK beta

   Plasmid

   #35405

   Depositor

   Stephen Prescott, Robert Lefkowitz

   Article

   Nelson et al Science. 2007 Feb 2;315(5812):663-6.

   Insert

   DGK beta (DGKB Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pGBW-m4046833

    Plasmid

    #145649

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp9

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp9 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. Nano-lantern-mitochondria/pcDNA3

    Plasmid

    #51973

    Purpose

    mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, targeted to mitochondria

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern
    

    Use

    Tags

    CoxVIIIx2

    Expression

    Mammalian

    Mutation

    S257G in Rluc8

    Promoter

    Available since

    March 28, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pQLinkHD_EBFP2-LSSmOrange

    Plasmid

    #118856

    Purpose

    fluorescent protein expression with an N-terminal His-tag

    Depositor

    Marc Birtwistle

    Article

    Holzapfel et al ACS Comb Sci. 2018 Nov 12;20(11):653-659

    Insert

    EBFP2-LSSmOrange
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    EBFP1.2-I128V/V150I/ D155V/V224R and for LSSmOra…

    Promoter

    Available since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTriEx4-H6-FGB

    Plasmid

    #49203

    Purpose

    Fluorescent reporter for cGMP

    Depositor

    John Garthwaite

    Article

    Bhargava et al Front Mol Neurosci. 2013 Sep 24;6:26. do

    Insert

    H6-FGB
    

    Use

    Tags

    His tag + Other tags

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    None

    Promoter

    CMV

    Available since

    Dec. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. DGK epsilon

    Plasmid

    #35407

    Depositor

    Stephen Prescott, Robert Lefkowitz

    Article

    Nelson et al Science. 2007 Feb 2;315(5812):663-6.

    Insert

    DGK epsilon (DGKE Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pZyxin-miRFP703

    Plasmid

    #79999

    Purpose

    Zyxin labeling with near-infrared fluorescent protein miRFP703

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

    Insert

    Zyxin (ZYX Human)
    

    Use

    Tags

    miRFP703

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-NHERF1-PDZ1iP

    Plasmid

    #28295

    Depositor

    Maria-Magdalena Georgescu

    Article

    Morales et al Mol Cell Biol. 2007 Apr . 27(7):2527-37.

    Insert

    NHERF1 (NHERF1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains aa 1-148 (deletion of PDZ2 and EB domain…

    Promoter

    Available since

    Sept. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pMB1-spect-PthrC-eGFP

    Plasmid

    #107408

    Purpose

    This plasmid contain promoter PthrC, which is used for study about Short, Auto-inducible Promoters for Well-Controlled Protein Expression in Escherichia coli.

    Depositor

    Kang Zhou

    Article

    Anilionyte et al Appl Microbiol Biotechnol. 2018 Aug;102(

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PthrC

    Available since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pXGFPC-5 Pxyl-dcas9 (Spa)

    Plasmid

    #133318

    Purpose

    expresses dcas9 (Streptococcus pasteurianus); repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xylose locus in Caulobacter crescentus; tetracycline resistance

    Depositor

    Michael Laub

    Article

    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

    Insert

    dcas9 (Streptococcus pasteurianus)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    xylose

    Available since

    Feb. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pActinin-miRFP670nano

    Plasmid

    #127432

    Purpose

    Alpha actinin labeling with near-infrared fluorescent protein

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

    Insert

    Alpha-actinin-miRFP670nano
    

    Use

    Tags

    miRFP670nano

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Nano-lantern(Ca2+)_300/pcDNA3

    Plasmid

    #51983

    Purpose

    mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=300nM

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern_CaM_E104Q-3GS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S257G in Rluc8

    Promoter

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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