We narrowed to 31,550 results for: Eng;

1. pSLQ9757: CMV-BPNLS_TadA-linkerA-TadA*(8e)-Linker B-SV40 NLS-dCasMINI-V4-linkerC-c-Myc_NLS-3x FLAG-polyA

   Plasmid

   #176270

   Purpose

   Expression of TadA-TadA* (8e)-dCasMINI-V4 in mammalian cells

   Depositor

   Stanley Qi

   Article

   Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

   Insert

   TadA-TadA*(8e)-dCasMINI-V4
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV.GFAP.(Cyto)iGlucoSnFR.mRuby2

   Plasmid

   #164514

   Purpose

   Green glucose sensor with mRuby2 fusion, cytosolic

   Depositor

   Loren Looger

   Article

   Keller et al bioRxiv (2019) 571422; doi: https://doi.

   Insert

   (Cyto)iGlucoSnFR-mRuby2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Synthetic Construct

   Promoter

   GFAP

   Available since

   Feb. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pYL-kasOp-FnCas12a3

   Plasmid

   #177875

   Purpose

   Expresses Streptomyces codon optimized FnCas12a. Used to modify genome of Streptomyces.

   Depositor

   Yunzi Luo

   Article

   Zhang et al Front Bioeng Biotechnol. 2020 Jun 30;8:7

   Insert

   Streptomyces codon optimized the FnCas12a mutant EP16
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   kasOp*

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV.GFAP.(Cyto)iGlucoSnFR

   Plasmid

   #164513

   Purpose

   Green glucose sensor, cytosolic

   Depositor

   Loren Looger

   Article

   Keller et al bioRxiv (2019) 571422; doi: https://doi.

   Insert

   (Cyto)iGlucoSnFR
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Synthetic Construct

   Promoter

   GFAP

   Available since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. IVT-R2Tg_opt

   Plasmid

   #223247

   Purpose

   In vitro transcription of the optimized R2Tg protein

   Depositor

   Wei Li

   Article

   Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

   Insert

   R2Tg protein_opt
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA_DENV2-NS2B3_V5

   Plasmid

   #115906

   Purpose

   Mammalian expression of DENV2 NS2B3 protein with a C-terminal V5 tag

   Depositor

   Alan Rothman

   Article

   Udawatte et al Front Cell Infect Microbiol. 2022 Oct 14

   Insert

   DENV2 NS2B3 protease
   

   Use

   Tags

   V5 tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. mCherry-INPP5K

   Plasmid

   #171950

   Purpose

   Expresses INPP5K in mammalian cells

   Depositor

   Cheng-han Yu

   Article

   Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

   Insert

   INPP5K (INPP5K Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. EF.hICN1.CMV.GFP

   Plasmid

   #17623

   Purpose

   Depositor

   Linzhao Cheng

   Article

   Yu et al Stem Cells. 2006 Apr . 24(4):876-88.

   Insert

   ICN1 (NOTCH1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pYL-kasOp-FnCas12a2

   Plasmid

   #177874

   Purpose

   Expresses Streptomyces codon optimized FnCas12a. Used to modify genome of Streptomyces.

   Depositor

   Yunzi Luo

   Article

   Zhang et al Front Bioeng Biotechnol. 2020 Jun 30;8:7

   Insert

   Streptomyces codon optimized FnCas12a
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   kasOp*

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mCherry-INPP5B

    Plasmid

    #171947

    Purpose

    Expresses INPP5B in mammalian cells

    Depositor

    Cheng-han Yu

    Article

    Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    INPP5B (INPP5B Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. OA984

    Plasmid

    #120363

    Purpose

    Expresses an single chain antibody 1C19 that targets all four DENV serotypes and expresses a red fluorescent protein reporter

    Depositor

    Omar Akbari

    Article

    Buchman et al PLoS Pathog. 2020 Jan 16;16(1):e1008103.

    Insert

    1C19 single chain antibody
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    AAEL010782 carboxypeptidase A (CPA) gene Promoter

    Available since

    Feb. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCDNA3.1-H-Luc_nucl

    Plasmid

    #113931

    Purpose

    Plasmid encoding the nuclear localization of the chimeric HaloTag7-NanoLucCP fusion H-Luc in mammalian cells for BRET

    Depositor

    Kai Johnsson

    Article

    Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

    Insert

    H-Luc_nucl
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCDNA3.1-H-Luc_cyto

    Plasmid

    #113929

    Purpose

    Plasmid encoding the cytosolic localization of the chimeric HaloTag7-NanoLucCP fusion H-Luc in mammalian cells for BRET

    Depositor

    Kai Johnsson

    Article

    Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

    Insert

    H-Luc
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. IVT-nCas9(H840A)

    Plasmid

    #207455

    Purpose

    in vitro transcription of mRNA

    Depositor

    Erik Sontheimer

    Article

    Liu et al Nat Biotechnol. 2023 Sep 14. doi: 10.103

    Insert

    H840A
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Tol2-lyzC-mito-Dendra2

    Plasmid

    #107590

    Purpose

    Expresses mitochondria localized Dendra2 in zebrafish neutrophils

    Depositor

    Qing Deng

    Article

    Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

    Insert

    mito-Dendra2
    

    Use

    Tags

    mitochondira localization sequence

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hsyn-flex-Positron-ST

    Plasmid

    #129267

    Purpose

    Cre-dependant expression of soma-localized Positron in neurons

    Depositor

    Eric Schreiter

    Article

    Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

    Insert

    Positron-ST
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    D92N, E199V

    Promoter

    hsyn

    Available since

    Oct. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p3E-U6a-polg guide

    Plasmid

    #107598

    Purpose

    An entry vector with U6a promoter driving polg guide RNA expression

    Depositor

    Qing Deng

    Article

    Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

    Insert

    polg guide RNA sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCDNA3.1-S-Luc_cyto

    Plasmid

    #113926

    Purpose

    Plasmid encoding the cytosolic localization of the chimeric SNAP-NanoLuc fusion S-Luc in mammalian cells for BRET

    Depositor

    Kai Johnsson

    Article

    Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

    Insert

    S-Luc
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. BPK2139

    Plasmid

    #65776

    Purpose

    Human expression vector for S aureus Cas9: CAG-humanSaCas9-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Staphylococcus aureus Cas9-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC-H1-gRNA

    Plasmid

    #61089

    Purpose

    CRISPR/Cas9 system gRNA cloning

    Depositor

    Shenglin Huang

    Article

    Zheng et al Biotechniques. 2014 Sep 1;57(3):115-24.

    Insert

    H1 promoter; gRNA sequences
    

    Use

    CRISPR; /cas9 grna cloning vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1

    Available since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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