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pCA0.001

Plasmid

#119557

Purpose

Level 0 acceptor vector for promoters truncated to transcriptional start site with lacZ compatible with blue/white screening

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046804

Plasmid

#145595

Purpose

Bacterial Expression plasmid for SARS-CoV-2 nsp8

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 nsp8 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;StrepII

Expression

Bacterial

Mutation

Escherichia coli recode 1

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046766

Plasmid

#145612

Purpose

Bacterial Expression plasmid for SARS-CoV-2 helicase

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 helicase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;StrepII

Expression

Bacterial

Mutation

Escherichia coli recode 1

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046802

Plasmid

#145683

Purpose

Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;HA

Expression

Bacterial

Mutation

wt

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pJUMP18-B0034-MV_RN

Plasmid

#127017

Purpose

Basic Part RN- RBS+N-terminus; pET vector Ribosome Binding Site + Met-Val N-terminus start.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part B0034m-MV_RN

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

None

Promoter

Available since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pDZ305 GTL1 GAL1p 24xPP7 V3 <HIS>

Plasmid

#72240

Purpose

Histidine selection; Used to insert a GAL1 promoter and 24 repetitive PP7 version 3 RNA stem-loops before GLT1's start codon (5' tagging)

Depositor

Daniel Zenklusen

Article

Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

Insert

GLT1 GAL1p 24xPP7 V3

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046897

Plasmid

#145579

Purpose

Bacterial Expression plasmid for SARS-CoV-2 ORF10 protein

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 ORF10 protein (ORF10 Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;HA

Expression

Bacterial

Mutation

wt

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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POM43-mCitrine

Plasmid

#69855

Purpose

N-terminal tagging with mCitrine using LEU2 as a selection marker in S. cerevisiae.

Depositor

Liza Pon

Article

Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

Insert

mCitrine minus start and stop codon

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 16, 2018

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046926

Plasmid

#145663

Purpose

Bacterial Expression plasmid for SARS-CoV-2 M (membrane)

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;HA

Expression

Bacterial

Mutation

Escherichia coli recode 1

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBT241_(pCA-ATG-intron-tTA2)

Plasmid

#36875

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Insert

tTA2

Use

Tags

Expression

Mammalian

Mutation

insertion of a beta-globin intron with a loxP sit…

Promoter

CAG (chicken beta actin promoter and CMV enhancer)

Available since

June 25, 2012

Availability

Academic Institutions and Nonprofits only

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pGBW-m4136453

Plasmid

#149535

Purpose

Bacterial expression plasmid for SARS-CoV-2 E (envelope)

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Use

Tags

6xHIS;Linker_GGSG;GST;Linker_GGSG;Cleavable TEV

Expression

Bacterial

Mutation

wt

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046744

Plasmid

#145728

Purpose

Bacterial Expression plasmid for SARS-CoV-2 ORF6 protein

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 ORF6 protein (ORF6 Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;FLAG_2

Expression

Bacterial

Mutation

wt

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046848

Plasmid

#149257

Purpose

Bacterial expression plasmid for SARS-CoV-2 nsp2

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 nsp2 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;StrepII

Expression

Bacterial

Mutation

wt

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

May 12, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046966

Plasmid

#145733

Purpose

Bacterial Expression plasmid for SARS-CoV-2 nsp6

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 nsp6 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;StrepII

Expression

Bacterial

Mutation

WT, AA 2-289

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046914

Plasmid

#145738

Purpose

Bacterial Expression plasmid for SARS-CoV-2 nsp9

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 nsp9 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;StrepII

Expression

Bacterial

Mutation

wt

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046839

Plasmid

#145646

Purpose

Bacterial Expression plasmid for SARS-CoV-2 3'-to-5' exonuclease

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 3'-to-5' exonuclease (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;StrepII

Expression

Bacterial

Mutation

wt

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pGBW-m4046813

Plasmid

#145619

Purpose

Bacterial Expression plasmid for SARS-CoV-2 helicase

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 helicase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;StrepII

Expression

Bacterial

Mutation

wt

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pGBW-m4046770

Plasmid

#145594

Purpose

Bacterial Expression plasmid for SARS-CoV-2 leader protein

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 leader protein (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;StrepII

Expression

Bacterial

Mutation

wt

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pGBW-m4046780

Plasmid

#145736

Purpose

Bacterial Expression plasmid for SARS-CoV-2 nsp10

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 nsp10 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;6xHIS

Expression

Bacterial

Mutation

wt

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

April 28, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046964

Plasmid

#145681

Purpose

Bacterial Expression plasmid for SARS-CoV-2 nsp3

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 nsp3 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;HA

Expression

Bacterial

Mutation

Escherichia coli recode 1

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge

Showing: 2261 - 2280 of 2760 results

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