We narrowed to 10,315 results for: plasmids 101

1. T7-AglK-His

   Plasmid

   #89714

   Purpose

   Expresses AglK from M. voltae with an N-terminal T7 tag and a C-terminal His6 tag. Plasmid has codons optimized for E. coli expression.

   Depositor

   Barbara Imperiali

   Article

   Zamora et al Methods Enzymol. 2017;597:145-186. doi:

   Insert

   AglK
   

   Use

   Tags

   His6 and T7

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSLC25A13-APEX2

   Plasmid

   #228198

   Purpose

   plasmid encoding human SLC25A13 with an APEX protein tag

   Depositor

   Stephen Benkovic

   Article

   Sha et al J Biol Chem. 2024 Aug 2;300(9):107620. d

   Insert

   SLC25A13 (SLC25A13 Human)
   

   Use

   Synthetic Biology

   Tags

   APEX2

   Expression

   Mutation

   Promoter

   M13

   Available Since

   Nov. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. SHLD1 N-terminal sgRNA

   Plasmid

   #207101

   Purpose

   pX330 based plasmid for expression of Cas9 and the ATGGCAGGACTATGGCAGCC sgRNA to target the SHLD1 locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   ATGGCAGGACTATGGCAGCC
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCm No carrier NikJC199U

   Plasmid

   #174361

   Purpose

   Expression plasmid with a TEV removable C-term 8xHis Tag and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.

   Depositor

   Brandon Greene

   Article

   Caceres et al Methods Enzymol. 2022;662:119-141. doi:

   Insert

   nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
   

   Use

   Tags

   8xHis and TEV

   Expression

   Bacterial

   Mutation

   C199U

   Promoter

   T7

   Available Since

   Dec. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRRG370-GS1

   Plasmid

   #99101

   Purpose

   dsRNA and riboprobe synthesis for Schmidtea mediterranea GS-1

   Depositor

   Phillip Newmark

   Article

   Roberts-Galbraith et al Elife. 2016 Sep 9;5. pii: e17002. doi: 1

   Insert

   GS-1 in situ probe
   

   Use

   T/a cloning vector for dsrna generation and the g…

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL2 (basic) 3xARE Lux

   Plasmid

   #14934

   Depositor

   Joan Massague

   Article

   Kretzschmar et al Genes Dev. 1999 Apr 1. 13(7):804-16.

   Insert

   3xARE
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 17, 2007

   Availability

   Academic Institutions and Nonprofits only

7. plRL19

   Plasmid

   #163634

   Purpose

   L5 Integrase delivery plasmid to allow integration of L5 attP only plasmids (e.g. plRL61, ID #163633).

   Depositor

   Jeremy Rock

   Article

   Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

   Insert

   L5 Integrase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Mycobacterial Optimized Promoter (MOP) and Tet-On

   Available Since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. plRL58

   Plasmid

   #166886

   Purpose

   Optimized M. tuberculosis CRISPRi plasmid; contains an L5 attP region (no L5 Integrase) that allows for integration when co-transformed with an L5 Integrase delivery plasmid (e.g. plRL19, ID #163634)

   Depositor

   Jeremy Rock

   Article

   Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Tet-On

   Available Since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. plRL61

   Plasmid

   #163633

   Purpose

   Optimized M. smegmatis CRISPRi plasmid; contains an L5 attP region (no L5 Integrase) that allows for integration when co-transformed with an L5 Integrase delivery plasmid (e.g. plRL19, ID #163634).

   Depositor

   Jeremy Rock

   Article

   Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Tet-On

   Available Since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV-F in pAR-9

    Plasmid

    #166921

    Purpose

    AAV rep/cap plasmid. Capsid is mouse brain tropic AAV-F

    Depositor

    Casey Maguire

    Article

    Hanlon et al Mol Ther Methods Clin Dev. 2019 Oct 23;1

    Insert

    REP/CAP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. mCherry-pCAG-mock

    Plasmid

    #228504

    Purpose

    This plasmid can be used for control experiments and can also be used as a plasmid backbone

    Depositor

    Wei Li

    Article

    Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-protein-mock

    Plasmid

    #228505

    Purpose

    This plasmid can be used for control experiments and can also be used as a plasmid backbone

    Depositor

    Wei Li

    Article

    Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGL2-GAL4-UAS-Luc

    Plasmid

    #33020

    Depositor

    Martin Walsh

    Article

    Nishio et al Proc Natl Acad Sci U S A. 2004 Aug 3;101

    Insert

    6X GAL4-UAS
    

    Use

    Tags

    Luciferase

    Expression

    Mutation

    Promoter

    Available Since

    April 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pROP-IP-GFP

    Plasmid

    #122136

    Purpose

    GFP Reporter plasmid for B-Rex contain imperfect operator sequence

    Depositor

    Srivatsan Raman

    Article

    Liu et al ACS Synth Biol. 2019 Feb 15;8(2):264-273

    Insert

    GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23101

    Available Since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pROP-PP-GFP

    Plasmid

    #122135

    Purpose

    GFP Reporter plasmid for B-Rex contain perfect operator sequence

    Depositor

    Srivatsan Raman

    Article

    Liu et al ACS Synth Biol. 2019 Feb 15;8(2):264-273

    Insert

    GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23101

    Available Since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. 4LG7

    Plasmid

    #60094

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    MBD4:JMC02A-C01:C228095 (MBD4 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available Since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

17. pYL116

    Plasmid

    #239967

    Purpose

    CRISPR donor plasmid with Puro-T2A-mCherry cassette for Suz12 disruption

    Depositor

    Thomas Cech

    Article

    Long et al Methods. 2021 Jul;191:44-58. doi: 10.101

    Insert

    PuroR-T2A-mCherry with homology arms for human Suz12
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYL117

    Plasmid

    #239968

    Purpose

    CRISPR donor plasmid with Puro-T2A-mCherry cassette for EZH2 disruption

    Depositor

    Thomas Cech

    Article

    Long et al Methods. 2021 Jul;191:44-58. doi: 10.101

    Insert

    PuroR-T2A-mCherry with homology arms for human Ezh2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLEX307-CTSB-puro

    Plasmid

    #158463

    Purpose

    Constitutive lentiviral expression of human CTSB.

    Depositor

    Sho Iketani, Alejandro Chavez

    Insert

    CTSB (CTSB Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLEX307-CTSB-blast

    Plasmid

    #158464

    Purpose

    Constitutive lentiviral expression of human CTSB.

    Depositor

    Sho Iketani, Alejandro Chavez

    Insert

    CTSB (CTSB Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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