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pICSL1P5_crU626

Plasmid

#191780

Purpose

Cas12a guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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BSA2_His

Plasmid

#202767

Purpose

bovine serum albumin design BSA2, in pET29b with C-terminal 6His-tag

Depositor

Sarel Fleishman

Article

Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

Insert

BSA2

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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BSA3_His

Plasmid

#202768

Purpose

bovine serum albumin design BSA3, in pET29b with C-terminal 6His-tag

Depositor

Sarel Fleishman

Article

Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

Insert

BSA1

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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475 p5EfliEBSbasP

Plasmid

#26027

Depositor

Nathan Lawson

Article

Villefranc et al Development. 2013 Apr;140(7):1497-506. d

Insert

fli1a intron 1

Available Since

Aug. 26, 2010

Availability

Academic Institutions and Nonprofits only

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C-term Hec1-targeted Aurora B FRET sensor

Plasmid

#45222

Depositor

Michael Lampson

Article

Liu et al J Cell Biol. 2010 Mar 22;188(6):809-20.

Insert

C-term Hec1-targeted Aurora B FRET sensor

Expression

Mammalian

Available Since

May 31, 2013

Availability

Academic Institutions and Nonprofits only

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pBT100.110

Plasmid

#122602

Purpose

clone CDA1 phage by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

CDA1

Expression

Bacterial

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pJ291_WT_med

Plasmid

#128856

Purpose

Medium copy number plasmid for expression of E. coli flagellin from an IPTG-inducible T5 promoter

Depositor

Mark Pallen

Article

Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

Insert

Flagellin

Expression

Bacterial

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX_307_ms2_VP64

Plasmid

#79375

Purpose

Recruits VP64 to compatible gRNAs for transcriptional activation

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

ms2-VP64

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-Sestrin2-S190W

Plasmid

#73670

Purpose

overexpression

Depositor

David Sabatini

Article

Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

Insert

Sesn2 (SESN2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

S190W changed serine 190 to tryptophan

Promoter

CMV

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only
258/-2.5lacZ

Plasmid

#25429

Depositor

Charles P. Emerson Jr.

Article

Goldhamer et al Development. 1995 Mar . 121(3):637-49.

Insert

Myo D Promoter (MYOD1 Human)

Use

Reporter construct

Tags

lacZ

Expression

Mammalian

Available Since

Aug. 2, 2010

Availability

Academic Institutions and Nonprofits only