Addgene

Showing: 2281 - 2300 of 6924 results

Results per page:

Sort results by:

pLenti-SF3B1-K700E-reporter

Plasmid

#200318

Purpose

A fluorescent reporter for SF3B1 K700E mutation. Co-expresses a blasticidin resistance gene for selection. Includes LoxP sites for Cre-mediated deletion of reporter cassette.

Depositor

Lili Wang

Article

Fernandez et al Cancer Res Commun. 2024 Aug 28. doi: 10.

Insert

BSD-2A-EGFPi

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1-a

Available since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AKT-translocation-mStrawberry reporter

Plasmid

#158685

Purpose

This plasmid is a AKT pathway reporter. It has 1EF1a promoter driving the expression of truncated FoxO1 fused to mStrawberry-pGK-BSD

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mStrawberry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiSAM v2 (Puro)

Plasmid

#92062

Purpose

Modified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

Depositor

Adam Karpf

Article

Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 (sgRNA) and EF1a (dCas9-VP64)

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-NPC1(WT)-FLAG

Plasmid

#164972

Purpose

Lentiviral vector for NPC1 expression in mammalian cells

Depositor

Roberto Zoncu

Article

Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

Insert

NPC1 (NPC1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF5-FRT-TagRFP-T-IFT88

Plasmid

#61684

Purpose

Mammalian expression of IFT88 fused with N terminal TagRFP.T tag

Depositor

Maxence Nachury

Article

Ye et al Elife. 2013 Aug 6;2:e00654. doi: 10.7554

Insert

IFT88 (Ift88 Mouse)

Use

Tags

TagRFP.T

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 6, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pXL001-TQM IL13 CAR

Plasmid

#154054

Purpose

Lentiviral expression of TQM IL13 CAR for CAR-T therapy

Depositor

Xiaojun Lian

Article

Kim et al Bioact Mater. 2020 May 7;5(3):624-635. d

Insert

TQM IL13 CAR

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pXG289

Plasmid

#141283

Purpose

OE of DELE1

Depositor

Martin Kampmann

Article

Guo et al Nature. 2020 Mar;579(7799):427-432. doi:

Insert

DELE1 (DELE1 Human)

Use

Tags

mClover

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-pCaMKII-tTAc

Plasmid

#172125

Purpose

Expression of InteinC-tTAC in CaMKII positive cells

Depositor

Chun Xu

Article

Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

Insert

pCaMKII, tTAc

Use

AAV

Tags

Expression

Mutation

Promoter

CaMKII

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-P2A_BAF_G47E

Plasmid

#101774

Purpose

Expresses mutant BAF (G47E) in human cells (with EGFP produced from the same transcript as expression control)

Depositor

Daniel Gerlich

Article

Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

Insert

BAF (BANF1) (BANF1 Human)

Use

Lentiviral

Tags

EGFP-P2A

Expression

Mammalian

Mutation

G47E mutation

Promoter

EF1a

Available since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAVTRE3_tdTomato

Plasmid

#135178

Purpose

AAV expression of TRE(TRE3G)-driven, tdTomato expression for fluorescent labling

Depositor

Tetsuo Yamamori

Article

Abe et al Front Neuroanat. 2018 Oct 30;12:89. doi:

Inserts

tdTomato
TRE3G

Use

AAV

Tags

Expression

Mammalian

Mutation

a variant of DsRed

Promoter

TRE3G

Available since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-P2A_BAF

Plasmid

#101773

Purpose

Expresses BAF in human cells (with EGFP produced from the same transcript as expression control)

Depositor

Daniel Gerlich

Article

Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

Insert

BAF (BANF1) (BANF1 Human)

Use

Lentiviral

Tags

EGFP-P2A

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
bArr1-V5

Plasmid

#201492

Purpose

Expression of the beta-arrestin1 containing a C-terminal V5 tag.

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

bArr1-V5 (ARRB1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-P2A_BAF_L58R

Plasmid

#101775

Purpose

Expresses mutant BAF (L58R) in human cells (with EGFP produced from the same transcript as expression control)

Depositor

Daniel Gerlich

Article

Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

Insert

BAF (BANF1) (BANF1 Human)

Use

Lentiviral

Tags

EGFP-P2A

Expression

Mammalian

Mutation

L58R mutation

Promoter

EF1a

Available since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMIA3-Pansio

Plasmid

#214445

Purpose

Expresses eSpCas9 and gRNA targeting Pansio-1 GSH

Depositor

Roger Foo

Article

Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

Insert

eSpCas9-P2A-mRuby2-P2A-tp53dn

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSH-EFIRES-P-AtAFB2-mCherry

Plasmid

#129716

Purpose

AAVS1 safe harbor targeting vector expressing AtAFB2-mCherry

Depositor

Elina Ikonen

Article

Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

Insert

AFB2 (AFB2 Mustard Weed)

Use

CRISPR and TALEN; Human safe harbor locus (a avs1…

Tags

mCherry

Expression

Mammalian

Mutation

codon optimized

Promoter

EF1a

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pRDA_831

Plasmid

#216100

Purpose

CRISPRa, EF1a-driven dCas9-VP64 (Cas only)

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

Cas9 [Sp]

Use

CRISPR and Lentiviral; Assembled vector

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pmCTIP2-N106

Plasmid

#66808

Purpose

2nd generation lentiviral transfer plasmid. Expresses mouse CTIP2 with blasticidin resistance

Depositor

Andrew Yoo

Article

Richner et al Nat Protoc. 2015 Oct;10(10):1543-55. doi

Insert

CTIP2 isoform b (Bcl11b Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Human EF1a

Available since

Sept. 21, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pMCB306-GFP-Rab8A

Plasmid

#198470

Purpose

lentiviral expression of GFP-Rab8A

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

GFP-Rab8A (RAB8A Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
dCas-hHDAC3-R265P

Plasmid

#103786

Purpose

Expresses dCas9 fused to human HDAC3 with R265P mutation

Depositor

Zhaolan Zhou

Article

Kwon et al Nat Commun. 2017 May 12;8:15315. doi: 10

Insert

dCas9-HDAC3-R265P (HDAC3 S. pyogenes, Human, Synthetic)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Changed arginine 265 to proline

Promoter

EF1A

Available since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
bArr2-V5

Plasmid

#201494

Purpose

Expression of the beta-arrestin2 containing a N-terminal V5 tag.

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

bArr2-V5 (ARRB2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge