We narrowed to 6,273 results for: nls
-
Plasmid#177681PurposeGateway entry clone (attL1 & attR5) for dimeric FokI-dCas9-SpRY genome editingDepositorInsertFokI-dCas9-SpRY
UseCRISPR; Gateway compatible foki-dcas9-spry entry …TagsNLSExpressionPlantAvailable SinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-NF1-L2-dSpRY
Plasmid#177682PurposeGateway entry clone (attL1 & attR5) for dimeric FokI-dCas9-SpRY genome editingDepositorInsertFokI-dCas9-SpRY
UseCRISPR; Gateway compatible foki-dcas9-spry entry …TagsNLSExpressionPlantAvailable SinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
lenti-dCas9-VP64-BFP
Plasmid#196711PurposeCRISPR-activation. dCas9-VP64 with TagBFP for sortingDepositorInsertdCas9-VP64-T2A-TagBFP
UseCRISPR and LentiviralTagsNucleoplasmin NLS and SV40 NLSMutationD10A, N863APromoterEF1aAvailable SinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
lenti-UCOE-dCas9-BFP-VP64
Plasmid#196717PurposeCRISPR-activation. dCas9-VP64 with TagBFP for sorting.DepositorInsertdCas9-TagBFP-VP64
UseCRISPR and LentiviralTagsNucleoplasmin NLS and SV40 NLSMutationD10A, N863APromoterEF1aAvailable SinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLH_Scr16
Plasmid#100538PurposeCEN/ARS plasmid with PhyBNT-NLS-CreN and PIF3-NLS-CreC expression cassettes for red/far-red light-regulated Cre recombinase activity in Saccharomyces cerevisiae.DepositorUseCre/Lox and Synthetic BiologyTagsCdc1 NLS, CreC, CreN, and SV40 NLSExpressionYeastMutationAA 1-621 of PhyB/ Mutation nucleotide 576 C-->APromoterFBA1 and TDH3Available SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pH-A3A-PBE
Plasmid#119774PurposeConversion of C to T in rice plants transformationDepositorInsertAPOBEC3A-nCas9-NLS-UGI-NLS
TagsNLS (flanking UGI)ExpressionPlantMutationnCas9 has a mutation D10A in wild type SpCas9Promotermaize Ubiquitin-1Available SinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pH-ePPE
Plasmid#183097PurposeFor plant prime editing in rice plantsDepositorInsertNls-nCas9-NC-nls-MLV-Nls
UseCRISPRExpressionPlantMutationD10A in SpCas9Available SinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pXR002: EF1a-dCasRx-2A-EGFP
Plasmid#109050PurposeCatalytically inactive dCasRx with 2A-EGFPDepositorInsertdCasRx
UseCRISPR and LentiviralTags2A-eGFP, HA, and NLSExpressionMammalianMutationR239A/H244A/R858A/H863AAvailable SinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
ePPE-spG
Plasmid#183096PurposeFor plant prime editing in rice plants or monocotyledons protoplastsDepositorInsertNls-nspGCas9-NC-nls-MLV-Nls
UseCRISPRExpressionPlantMutationD10A in SpGCas9Available SinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
BPK2139
Plasmid#65776PurposeHuman expression vector for S aureus Cas9: CAG-humanSaCas9-NLS-3xFLAGDepositorInsertmammalian codon-optimized Staphylococcus aureus Cas9-NLS-3xFlag
UseCRISPRTagsNLS-3xFLAGExpressionMammalianPromoterCAGAvailable SinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
SQT1665
Plasmid#78744PurposeHuman expression plasmid for LbCpf1-NLS-3xHADepositorInsertmammalian codon-optimized Lachnospiraceae bacterium ND2006 Cpf1-NLS-3xHA
UseCRISPRTagsNLS-3xHAExpressionMammalianPromoterCAGAvailable SinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
ePPE
Plasmid#183095PurposeFor plant prime editing in rice plants or monocotyledons protoplastsDepositorInsertNls-nCas9-NC-nls-MLV-Nls
UseCRISPRExpressionPlantMutationD10A in SpCas9Available SinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Nme2Cas9_pMCSG7
Plasmid#119925PurposeNme2Cas9 bacterial expression plasmidDepositorInsertNme2Cas9
TagsNLS, Nucleoplasmin NLS, and SV40 NLSExpressionBacterialPromoterT7Available SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
SQT1659
Plasmid#78743PurposeHuman expression plasmid for AsCpf1-NLS-3xHADepositorInsertmammalian codon-optimized Cpf1-NLS-3xHA
UseCRISPRTagsNLS-3xHAExpressionMammalianPromoterCAGAvailable SinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
A3A-PBE
Plasmid#119768PurposeConversion of C to T in wheat plants or monocotyledons protoplastsDepositorInsertAPOBEC3A-nCas9-NLS-UGI-NLS
TagsNLS (flanking UGI)ExpressionPlantMutationnCas9 has a mutation D10A and E945K in wild type …Promotermaize Ubiquitin-1Available SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
p35S-A3A-PBE
Plasmid#119773PurposeConversion of C to T in potatoDepositorInsertAPOBEC3A-nCas9-NLS-UGI-NLS
TagsNLS (flanking UGI)ExpressionPlantMutationnCas9 has a mutation D10A in wild type SpCas9PromoterCaMv 2x35SAvailable SinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
MSP1594
Plasmid#65775PurposeHuman expression vector for S. thermophilus 1 Cas9: CAG-humanSt1Cas9-NLSDepositorInsertmammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS
UseCRISPRTagsNLSExpressionMammalianPromoterCAGAvailable SinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
A3A-Gam
Plasmid#119769PurposeConversion of C to T monocotyledons protoplastsDepositorInsertGam-APOBEC3A-nCas9-NLS-2XUGI-NLS
TagsNLS (flanking UGI)ExpressionPlantMutationnCas9 has a mutation D10A in wild type SpCas9Promotermaize Ubiquitin-1Available SinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR.v2.TetON_Cas13d_U6_DR
Plasmid#196727PurposeSortable and selectable, Tet-inducible RfxCas13d with guide cassette (one vector system). Inducible knock-down.DepositorInsertRfxCas13d-T2A-miRFP670nano
UseCRISPR and LentiviralTagsNucleoplasmin NLS and SV40 NLSPromoterTREAvailable SinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
lenti-dCas9-ZIM3-KRAB-BFP
Plasmid#196712PurposeCRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3DepositorInsertdCas9-KRAB(ZIM3)-T2A-TagBFP
UseCRISPR and LentiviralTagsNucleoplasmin NLS and SV40 NLSMutationD10A, N863APromoterEF1aAvailable SinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only