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pLV6-Bmal-luc
Plasmid#68833PurposeExpression of Bmal1-luc in mammalian cellsDepositorInsertBmal1 regulatory sequences (Bmal1 Mouse)
UseLentiviral and LuciferaseTagsluciferaseExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFLAG_WT MDA5
Plasmid#216798PurposeExpresses wild type (wt) melanoma differentiation-associated protein 5DepositorInsertmelanoma differentiation-associated protein 5 (IFIH1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC_cohNaV1.1_2009aa_IRES2_GFP
Plasmid#227642PurposeExpresses Nav 1.1 and IRES-GFPDepositorInsertNaV1.1 (SCN1A Human)
UseTagsIRES-GFPExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hNurr1-NE
Plasmid#102363PurposeOverexpression of NR4A2 nuclear receptor subfamily 4 group A member 2 in mammalian cellsDepositorInsertNR4A2 nuclear receptor subfamily 4 group A member 2 (NR4A2 Human)
UseTagsNEExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAVone-AAV2-CMV-eGFP
Plasmid#230930PurposepAAVone plasmid is used to package AAV-CMV-eGFP with an AAV2 serotype, utilizing the AAVone single-plasmid AAV production system.DepositorInserteGFP
UseAAVTagsExpressionMutationPromoterCMVAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHR_SFFV*/Puro2a-GFP
Plasmid#198222PurposeLentiviral plasmid derived from pHR_SFFV (Addgene #79121) expressing cDNA for a codon-optimized puromycin resistance gene, followed by an FMDV 2A domain and then EGFPDepositorInsertEGFP
UseLentiviralTagsCodon-optimized puromycin resistance gene, follow…ExpressionMammalianMutationPromoterAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)
Plasmid#140082PurposeEntry cloning vector for in vitro transcription or expression of SpCas9 sgRNAs from a T7 promoterDepositorInsertpT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)
UseIn vitro transcription (mrna synthesis)TagsExpressionBacterialMutationPromoterT7Available sinceOct. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFLAG_G495R MDA5
Plasmid#216799PurposeExpresses a single point mutant of melanoma differentiation-associated protein 5DepositorInsertmelanoma differentiation-associated protein 5 (IFIH1 Human)
UseTagsExpressionMammalianMutationG495RPromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-hM4D(Gi)-mCherry
Plasmid#50475PurposeGi-coupled hM4D DREADD fused with mCherry under the control of human synapsin promoter.DepositorHas ServiceAAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InserthM4D(Gi)-mCherry
UseAAVTagsmCherryExpressionMutationSee supplemental documents for DREADD mutationsPromoterhuman Synapsin 1Available sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn GFP-Cre
Plasmid#175381Purposeexpresses GFP-Cre under the control of a human synapsin promoterDepositorInsertGFP-Cre recombinase
UseAAVTagsCre is fused to GFP. GFP is fused to additional n…ExpressionMutationPromoterhuman synapsinAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21a-alpha-synuclein
Plasmid#51486PurposeInducible expression of human alpha-synuclein in E.coliDepositorInsertalpha-synuclein (SNCA Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 31, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pKAN43Ag8-GFP
Plasmid#178182PurposePlant binary vector expressing GFP by a enhanced 35S promoterDepositorInsertEGFP (eGFP )
UseTagsExpressionPlantMutationThe CAT is inserted after the start codon.PromoterThe minimal 35S promoter from Cauliflower Mosaic …Available sinceSept. 26, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCDNA3 HA C9ORF72 op
Plasmid#74156PurposeExpress HA tagged optimized C9ORF72 in mammalian cellsDepositorInsertC9ORF72 (C9orf72 Human, Synthetic)
UseTagsHAExpressionMammalianMutationoptimized sequencePromoterAvailable sinceApril 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pD454-SR mouse Alpha-synuclein
Plasmid#89075Purposebacterial expression of mouse Alpha-synucleinDepositorInsertAlpha-synuclein (Snca Mouse)
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 17, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
lenti-UCOE-dCas9-BFP-Zim3-KRAB
Plasmid#196716PurposeCRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3DepositorInsertdCas9-TagBFP-KRAB(ZIM3)
UseCRISPR and LentiviralTagsNucleoplasmin NLS and SV40 NLSExpressionMutationD10A, N863APromoterEF1aAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQXCINeo-LOX
Plasmid#134761Purposea retroviral vector to express lysyl oxidase (derived from PCR4-TOPO-LOX)DepositorInsertLOX (LOX Human)
UseRetroviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSNAPf-H2B Control Plasmid
Plasmid#101124PurposeExpression of SNAP-tagged Histone H2B in mammalian cellsDepositorInserthistone cluster 1 H2B family member j (H2BC11 Human)
UseTagsSNAP-tag (SNAPf)ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCambia-loxKan
Plasmid#199590Purposebasic plant vector with a plant-selectable kanamycin resistance cassette flanked by both loxP and FRT sites for sustainable plant engineering with subsequent marker removal possibilityDepositorInsertFRT-lox66-NosP-KanR-NosT-lox71-FRT
UseCre/LoxTagsExpressionPlantMutationPromoterNosAvailable sinceJuly 5, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPB-TREG3G-PE2-rtTA3G-P2A-eGFP
Plasmid#196971PurposePiggybac vector with doxycyclin-inducible prime editor for mammalian cellsDepositorInsertsCas9-RT
rtTA3G-P2A-eGFP
UseTagsExpressionMammalianMutationPromoterPGK and TREG3GAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET50_WT MDA5
Plasmid#216802PurposeExpresses wild type (wt) melanoma differentiation-associated protein 5DepositorInsertmelanoma differentiation-associated protein 5 (IFIH1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
human EB1 in pEGFP N1 to express EB1-GFP (JB131)
Plasmid#39299DepositorInsertEB1 (MAPRE1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-N-HA
Plasmid#119756PurposeAllows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal HA tag.DepositorTypeEmpty backboneUseTagsGST and HAExpressionBacterialMutationPromotertac promoterAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFLAG-CMV-hErk1
Plasmid#49328PurposeExpresses FLAG-tagged human Erk1DepositorInsertErk1 (MAPK3 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-DEST
Plasmid#119749PurposeAllows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag.DepositorTypeEmpty backboneUseTagsGSTExpressionBacterialMutationPromotertac promoterAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAVone-AAV9-CMV-eGFP
Plasmid#230928PurposepAAVone plasmid is used to package AAV-CMV-eGFP with an AAV9 serotype, utilizing the AAVone single-plasmid AAV production system.DepositorInserteGFP
UseAAVTagsExpressionMutationPromoterCMVAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLVX-FLAG-mCherry-C1-centrin-1
Plasmid#73333PurposeLentiviral vector encoding centrin-1 with N-terminal FLAG and mCherry tagsDepositorInsertcentrin-1 (CETN1 Human)
UseLentiviralTagsFLAG and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro U6 sgRNA BfuAI large stuffer
Plasmid#52628Purposelentiviral U6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sites, improved cassette cloning efficiencyDepositorInsertKanamycin cassette
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFGC-pcoCas9
Plasmid#52256PurposeA binary plasmid containing 35SPPDK:pcoCas9:NOS cassette for plant expression of Cas9 and multiple cloning sites for insertion of guide RNADepositorInsert35SPPDK:pcoCas9:NOS cassette
UseCRISPR; Plant expressionTagsExpressionMutationPromoterconstitutive 35SPPDK promoterAvailable sinceMarch 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG/HA-hBIG2
Plasmid#226254PurposeExpresses N-terminally HA-tagged hBIG2 in mammalian cellsDepositorInsertBIG2 (ARFGEF2 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET23a_mod_HuD_FL
Plasmid#65751Purposeplasmid for bacterial overexpression of HuDDepositorInsertELAVL4 (ELAVL4 Human)
UseTags6X His and FLAG HAExpressionBacterialMutationPromoterT7Available sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-hSyn-con/fon DREADD Gq-mCherry
Plasmid#200661PurposeConstruct for chemogenetically activating and visualizing subpopulations of neurons in the retinaDepositorInsertDREADD Gq-mCherry
UseAAV, Cre/Lox, and Mouse Targeting ; IntrsectTagsmCherryExpressionMammalianMutationPromoterhSynAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
human Alpha-synuclein
Plasmid#51437Purposehuman codon optimized Alpha-synucleinDepositorInsertAlpha-synuclein (SNCA Human)
UseCloningTagsExpressionMutationPromoterAvailable sinceMarch 31, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
miniTurbo-dCasRx
Plasmid#219821PurposeEF1α-driven expression of miniTurbo fused to dCasRx with GFP (Adapted from plasmid #153209)DepositorInsertdCasRx
UseCRISPRTagsminiTurboExpressionMammalianMutationPromoterEF1a and hU6Available sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CB-Nluc-P2A-copGFP-T2A-Puro
Plasmid#73039PurposeExpress Nluc, copGFP dual reporterDepositorInsertNluc-P2A-copGFP-T2A-Puro
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterchicken beta-actin + CMV enhancer and chicken bet…Available sinceJan. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUS250
Plasmid#198322PurposeCumate-inducible gene expression in diverse gram negative bacteria. Features also include mobilisation via oriT, a gigantic multiple cloning site, and blue/white screening via amilCP chromoproteinDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromotercumate-inducibleAvailable sinceApril 12, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET-24a-ELP[V-30]
Plasmid#67014PurposeExpresses ELP[V-30] in E. coliDepositorInsertELP[V-30]
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDH-Flag-hS1pr1-GFP-Neo
Plasmid#203708PurposeOverexpression of FLAG-S1PR1-GFP fusion.DepositorInsertFlag-hS1PR1-GFP (S1PR1 Human)
UseLentiviralTagsFlag and GFPExpressionMammalianMutationWTPromoterCMVAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
human EB1 fused to monomeric RFP
Plasmid#39323DepositorInsertsUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only
