Addgene

We narrowed to 4,649 results for: INV-E

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pOVC1_X_EcSSPB-nano

Plasmid

#131219

Purpose

Expresses EcSSPB-micro (Escherichia coli) preceded by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

EcSSPB-nano

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pOVC1_EcSSPB-micro_X

Plasmid

#131213

Purpose

Expresses EcSSPB-micro (Escherichia coli) followed by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

EcSSPB-micro

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF1H11

Plasmid

#70853

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Taf7 (Taf7 Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only
pCDNA3-Flag-SPRTN-wt

Plasmid

#110214

Purpose

Expression in mammalian cells of full-length wild-type SPRTN protein with a Flag-tag at N-terminus.

Depositor

Kristijan Ramadan, Ivana Marinovic-Terzic

Article

Lessel et al Nat Genet. 2014 Nov;46(11):1239-44. doi:

Insert

SPRTN (SPRTN Human)

Use

Tags

Expression

Mammalian

Mutation

P296L (see depositor comments below)

Promoter

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3-Flag-SPRTN-E112A

Plasmid

#110215

Purpose

Expression in mammalian cells of of full-length SPRTN protein carrying E112A mutation, a catalytically-dead mutant, and Flag-tag at N-terminus.

Depositor

Kristijan Ramadan, Ivana Marinovic-Terzic

Article

Lessel et al Nat Genet. 2014 Nov;46(11):1239-44. doi:

Insert

SPRTN (SPRTN Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

E112A, P296L (see depositor comments below)

Promoter

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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LacZ-5BoxB in pcDNA3.1+

Plasmid

#29729

Depositor

Howard Chang

Article

Wang et al Nature. 2011 Mar 20. ():.

Insert

LacZ-5BoxB

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 23, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3-V5-DGCR8

Plasmid

#51383

Depositor

Narry Kim

Article

Yeom et al Nucleic Acids Res. 2006;34(16):4622-9. E

Insert

DGCR8 (DGCR8 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-subtilase A

Plasmid

#35948

Depositor

Zhaoyang Feng

Article

Yuan et al PLoS One. 2011;6(8):e22354. Epub 2011 Au

Insert

Subtilase A (subA E. Coli)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 14, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-mNox1

Plasmid

#58340

Purpose

expresses mouse Nox1 in mammalian cells

Depositor

Botond Banfi, Karl-Heinz Krause

Article

Banfi et al J Biol Chem. 2003 Feb 7;278(6):3510-3. E

Insert

Mus musculus NADPH oxidase 1 alpha (Nox1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

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p3E-Dam-myc no-pA

Plasmid

#82600

Purpose

3' entry vector containing myc epitope tag fused to C-terminus of E. coli DNA adenine methyltransferase without pA for DamID

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

Dam-Myc (no pA)

Use

Tags

Expression

Mutation

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV Lex VP16 (F442A) HA (P#1710)

Plasmid

#14595

Depositor

Matija Peterlin

Article

Kurosu et al Curr Biol. 2004 Jun 22. 14(12):1112-6.

Insert

LexA

Use

Tags

HA and VP16 (F442A)

Expression

Mammalian

Mutation

The VP16 sequence contains an F442A mutation. The…

Promoter

Available since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
pCMV Ub Lex VP16 (F442A) HA (P#1711)

Plasmid

#14596

Depositor

Matija Peterlin

Article

Kurosu et al Curr Biol. 2004 Jun 22. 14(12):1112-6.

Insert

LexA

Use

Tags

HA, Ubiquitin, and VP16 (F442A)

Expression

Mammalian

Mutation

The VP16 sequence contains an F442A mutation. The…

Promoter

Available since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
KHBD00334

Plasmid

#34457

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG11491 (br Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pBad-sfGFP 150TAG

Plasmid

#85483

Purpose

Arabinose inducible E. coli codon optimized superfolder GFP with TAG stop codon at position 150

Depositor

Ryan Mehl

Article

Miyake-Stoner et al Biochemistry. 2010 Mar 2;49(8):1667-77.

Insert

superfolder GFP 150TAG

Use

Tags

Expression

Bacterial

Mutation

150TAG (amino acid 150 mutated to stop codon)

Promoter

Arabinose

Available since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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T7-His-GFP-Arp11

Plasmid

#51402

Purpose

expression of GFP-tagged mouse Arp11 under T7 promoter

Depositor

Trina Schroer

Article

Eckley et al Mol Biol Cell. 2003 Jul;14(7):2645-54. E

Insert

Arp11 (Actr10 Mouse)

Use

Tags

GFP and His

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 5, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-V5-DGCR8-mDRBD1

Plasmid

#51384

Depositor

Narry Kim

Article

Yeom et al Nucleic Acids Res. 2006;34(16):4622-9. E

Insert

DGCR8 (DGCR8 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

DRBD1 mutant (A568K, A569K)

Promoter

CMV

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-V5-DGCR8-mDRBD2

Plasmid

#51386

Depositor

Narry Kim

Article

Yeom et al Nucleic Acids Res. 2006;34(16):4622-9. E

Insert

DGCR8 (DGCR8 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

DRBD2 mutant (A676K, S677K)

Promoter

CMV

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only
T7-His-Arp11(1–417)

Plasmid

#51400

Purpose

expression of full-length mouse Arp11 under T7 promoter

Depositor

Trina Schroer

Article

Eckley et al Mol Biol Cell. 2003 Jul;14(7):2645-54. E

Insert

Arp11 (Actr10 Mouse)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 20, 2014

Availability

Academic Institutions and Nonprofits only
pDEST26-C-FLAG

Plasmid

#79275

Purpose

Destination vector containing a C-terminal FLAG tag

Depositor

Rita Shiang

Article

Amr et al Am J Hum Genet. 2007 Oct;81(4):673-83. E

Insert

FLAG

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 13, 2017

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDEST26-N-FLAG

Plasmid

#79549

Purpose

Destination vector containing an N-terminal FLAG tag

Depositor

Rita Shiang

Article

Amr et al Am J Hum Genet. 2007 Oct;81(4):673-83. E

Insert

FLAG

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 13, 2017

Availability

Industry, Academic Institutions, and Nonprofits
AiP5003 pAAV-Syn-DIO-TVA66T-dTom-CVS-N2cG

Plasmid

#176285

Purpose

Cre-dependent AAV virus

Depositor

The Allen Institute for Brain Science, Shenqin Yao

Article

Yao et al Nat Neurosci. 2023 Feb;26(2):350-364. do

Insert

TVA66T-dTomato-rabies Glycoprotein

Use

Tags

dTomato

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP5004 pAAV-Syn-DIO-TVA-dTom-SAD-B19G

Plasmid

#176286

Purpose

Cre-dependent AAV virus

Depositor

The Allen Institute for Brain Science, Shenqin Yao

Article

Yao et al Nat Neurosci. 2023 Feb;26(2):350-364. do

Insert

TVA-dTomato-rabies Glycoprotein

Use

Tags

dTomato

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP5005 pAAV-Syn-DIO-TVA66T-dTom-SAD-B19G

Plasmid

#176287

Purpose

Cre-dependent AAV virus

Depositor

The Allen Institute for Brain Science, Shenqin Yao

Article

Yao et al Nat Neurosci. 2023 Feb;26(2):350-364. do

Insert

TVA66T-dTomato-rabies Glycoprotein

Use

Tags

dTomato

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
myc-BioID-Rab7

Plasmid

#121049

Purpose

Expresses myc tagged BioID and human Rab7a in mammalian cells

Depositor

Gia Voeltz

Article

Hoyer et al Cell. 2018 Sep 20;175(1):254-265.e14. do

Inserts

BioID
Rab7A

Use

Tags

myc

Expression

Mammalian

Mutation

R118G (highly promiscuous form)

Promoter

CMV

Available since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-V5-DGCR8-mDRBD1&2

Plasmid

#51385

Depositor

Narry Kim

Article

Yeom et al Nucleic Acids Res. 2006;34(16):4622-9. E

Insert

DGCR8 (DGCR8 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

DRBD1 & DRBD2 mutant (A568K, A569K, A676K, S6…

Promoter

CMV

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only
CFP(1-158) in pcDNAI/Amp

Plasmid

#60895

Purpose

This vector attaches CFP(1-158) to the N-terminus of a protein.

Depositor

Catherine Berlot

Article

Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E

Insert

CFP(1-158)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA6-ARID1A

Plasmid

#39311

Depositor

Ie-Ming Shih

Article

Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. E

Insert

ARID1A (ARID1A Human)

Use

Tags

His and V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 13, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 MDM2 1-400

Plasmid

#45754

Depositor

Robert Lefkowitz

Article

Shenoy et al Science. 2001 Nov 9;294(5545):1307-13. E

Insert

MDM2 1-400 (MDM2 Human)

Use

Tags

Expression

Mammalian

Mutation

aa 1-400, lacks catalytic RING domain

Promoter

CMV

Available since

Aug. 21, 2013

Availability

Academic Institutions and Nonprofits only
CFP(159-238) in pcDNAI/Amp

Plasmid

#60896

Purpose

This vector attaches CFP(159-238) to the N-terminus of a protein.

Depositor

Catherine Berlot

Article

Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E

Insert

CFP(159-238)

Use

Tags

Expression

Mammalian

Mutation

Asn-164 to His

Promoter

CMV

Available since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEP4-hKCNE1

Plasmid

#53050

Purpose

Expresses beta subunit for IKs channel in mammalian cells

Depositor

Michael Sanguinetti

Article

Sanguinetti et al Nature. 1996 Nov 7;384(6604):80-3.

Insert

Potassium voltage-gated channel subfamily E member 1 (KCNE1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pAC-luc-TimRA

Plasmid

#33133

Depositor

Michael Rosbash

Article

Kadener et al Genes Dev. 2009 Sep 15;23(18):2179-91. E

Insert

Tim-RA 3' UTR (tim Fly)

Use

Luciferase

Tags

Firefly Luciferase

Expression

Insect

Mutation

Promoter

Actin 5C

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pRSET-6xTR-TUBE

Plasmid

#110313

Purpose

E. coli expression and purification of 6xTR-TUBE

Depositor

Yasushi Saeki

Article

Tsuchiya et al Nat Commun. 2018 Feb 6;9(1):524. doi: 10

Insert

6xTR-TUBE (UBQLN1 Human)

Use

Tags

N-terminal His6-T7 tag

Expression

Bacterial

Mutation

All Arg residues in the UBA domain are mutated to…

Promoter

T7

Available since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSET-4xTR-TUBE

Plasmid

#110312

Purpose

E. coli expression and purification of 4xTR-TUBE

Depositor

Yasushi Saeki

Article

Tsuchiya et al Nat Commun. 2018 Feb 6;9(1):524. doi: 10

Insert

4xTR-TUBE (UBQLN1 Human)

Use

Tags

N-terminal His6-T7 tag

Expression

Bacterial

Mutation

All Arg residues in the UBA domain are mutated to…

Promoter

T7

Available since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3.1Xpress-Bcr-HisB

Plasmid

#32508

Depositor

Nora Heisterkamp, John Groffen

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

BCR (BCR Human)

Use

Tags

HISB

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 26, 2012

Availability

Academic Institutions and Nonprofits only
pCDNA3.1Xpress-BcrR1090A-HisB

Plasmid

#32507

Depositor

Nora Heisterkamp, John Groffen

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

BCR (BCR )

Use

Tags

HISB

Expression

Mammalian

Mutation

R 1090 to A

Promoter

Available since

Jan. 26, 2012

Availability

Academic Institutions and Nonprofits only
Cerulean(1-158) in pcDNAI/Amp

Plasmid

#60897

Purpose

This vector attaches Cerulean(1-158) to the N-terminus of a protein.

Depositor

Catherine Berlot

Article

Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E

Insert

Cerulean(1-158)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA6-ARID1A 1–1758

Plasmid

#39475

Depositor

Ie-Ming Shih

Article

Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. E

Insert

ARID1A (ARID1A Human)

Use

Tags

His and V5

Expression

Mammalian

Mutation

contains aa1–1758

Promoter

CMV

Available since

Sept. 13, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA6-ARID1A 1759-2285

Plasmid

#39476

Depositor

Ie-Ming Shih

Article

Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. E

Insert

ARID1A (ARID1A Human)

Use

Tags

His and V5

Expression

Mammalian

Mutation

expresses ARID1A aa1759-2285

Promoter

Available since

Sept. 13, 2012

Availability

Academic Institutions and Nonprofits only
pFB-GTE-COI1

Plasmid

#29516

Depositor

Ning Zheng

Article

Sheard et al Nature. 2010 Nov 18. 468(7322):400-5.

Insert

COI1 (COI1 Mustard Weed)

Use

Tags

GST and TEV cleavage site

Expression

Insect

Mutation

Note that the first AA in TEV site is R, not E

Promoter

Available since

May 26, 2011

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf4

Plasmid

#12692

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jon Yewdell, Jack Bennink

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

C ORF E

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 1, 2006

Availability

Academic Institutions and Nonprofits only

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