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We narrowed to 15,323 results for: pET

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Showing: 4561 - 4600 of 15323 results

  1. 371-R

    Plasmid
    #97033
    Purpose
    StrepII-tev-yORF
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Tags
    StrepII tag and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    97033-plasmid-map-sequence-id-182501

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  2. 371-S

    Plasmid
    #97034
    Purpose
    His6-Sumo-tev-yORF
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Tags
    His6 tag, SUMO domain, and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    97034-plasmid-map-sequence-id-182498

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  3. 371-O

    Plasmid
    #97032
    Purpose
    His6-Mocr-tev-yORF
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Tags
    His6 tag, TEV cleavage site, and mOCR fusion
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    97032-plasmid-map-sequence-id-182497

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  4. 371-L

    Plasmid
    #97031
    Purpose
    FLAG-tev-yORF
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Tags
    FLAG tag and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    97031-plasmid-map-sequence-id-182492

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  5. pCJ136

    Plasmid
    #162665
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162665-plasmid-map-sequence-id-321451

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  7. pCJ207

    Plasmid
    #162680
    Purpose
    pET-21b(+) based plasmid for expression of the putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with C-terminal His tag, codon optimized for expression in E. coli K12.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    Putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with signal peptide
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162680-plasmid-map-sequence-id-321455

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  8. pCJ199

    Plasmid
    #162672
    Purpose
    pET-21b(+) based plasmid for expression of the putative MHET hydrolase from Comamonas thiooxydans (Genbank WP_080747404.1) with C-terminal His tag, codon optimized for expression in E. coli K12.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    Putative MHET hydrolase from Comamonas thiooxydans (Genbank WP_080747404.1) with signal peptide
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162672-plasmid-map-sequence-id-321466

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  9. pCJ203

    Plasmid
    #162678
    Purpose
    pET-21b(+) based plasmid for expression of the putative MHET hydrolase from Comomonas thiooxydans (Genbank WP_080747404.1) with deletion of the predicted signal peptide (Phe2:Ala75)
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    Putative MHETase from Comomonas thiooxydans (Genbank WP_080747404.1) without 75 residue signal peptide
    Tags
    His
    Expression
    Bacterial
    Mutation
    deltaF2-A75; codon optimized for expression in E.…
    Promoter
    T7/lac
    Available Since
    Feb. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    162678-plasmid-map-sequence-id-321524

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  10. pMSP1D1Q1

    Plasmid
    #97326
    Purpose
    Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional Q residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.
    Depositor
    Michael Marty
    Insert
    MSP1D1Q1
    Tags
    6xHis - TEV
    Expression
    Bacterial
    Mutation
    Addition of Q residue after T25
    Promoter
    T7
    Available Since
    Aug. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    97326-plasmid-map-sequence-id-182622

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  11. pMH_MBP_AtCK2beta1_95-287

    Plasmid
    #238519
    Purpose
    Bacterial expression of A. thaliana CK2beta1 95-287
    Depositor
    Michael Hothorn
    Insert
    AtCK2beta1 (CKB1 Mustard Weed)
    Tags
    2x Strep, 6x His, MBP, N-term, TEV cleavable
    Expression
    Bacterial
    Available Since
    June 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    238519-plasmid-map-sequence-id-477135

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  12. CAPN2A-c002

    Plasmid
    #205104
    Purpose
    CAPN2 protein expression
    Depositor
    Paul Brennan
    Insert
    CAPN2 (CAPN2 Human)
    Tags
    6His-TEV and AviTag
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Oct. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    205104-plasmid-map-sequence-id-412141

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  13. PRPF4.001.169.S34E

    Plasmid
    #137643
    Purpose
    Expresses N-terminal (His)6-TEV cleavage site fusion of human gene or portion of gene, with point mutation, in bacterial strains. pET based vector.
    Depositor
    Tara Davis
    Insert
    PRPF4 (PRPF4 Human)
    Tags
    (His)6-TEV
    Expression
    Bacterial
    Mutation
    001-169-S34E
    Available Since
    Jan. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    137643-plasmid-map-sequence-id-381376

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  14. PRPF4.211.522.gst

    Plasmid
    #137650
    Purpose
    Expresses N-terminal GST-(His)6-Thrombin cleavage site fusion of human gene or portion of gene in bacterial strains. pET based vector.
    Depositor
    Tara Davis
    Insert
    PRPF4 (PRPF4 Human)
    Tags
    GST-(His)6-thrombin
    Expression
    Bacterial
    Mutation
    211-522-gst
    Available Since
    Nov. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    137650-plasmid-map-sequence-id-377729

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  15. KSP_HNL

    Plasmid
    #129772
    Purpose
    Expresses Xenopus Kinesin-5 KSP motor and neck linker domain monomer for biochemical assays
    Depositor
    William Hancock
    Insert
    KSP (kif11.L Frog)
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Truncation at 367
    Available Since
    Dec. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    129772-plasmid-map-sequence-id-264857

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  16. 371-C

    Plasmid
    #97029
    Purpose
    His6-MBP-N10-tev-yORF
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Tags
    His6 tag, MBP domain, N10 linker, and TEV cleavag…
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    97029-plasmid-map-sequence-id-182500

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  17. pSMH1741

    Plasmid
    #160930
    Purpose
    Expression of soluble Sc octamer (H3)
    Depositor
    Stephen Harrison
    Inserts
    HHF1
    HTA1
    HTB1
    HHT1
    Tags
    6His-TEV, N-6His-TEV, N-terminal 6His-TEV, and no…
    Mutation
    codon-optimized for E. coli -- see provided seque…
    Available Since
    Dec. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    160930-plasmid-map-sequence-id-318962

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  18. pSMH1742

    Plasmid
    #160929
    Purpose
    Expression of soluble Sc octamer (Cse4)
    Depositor
    Stephen Harrison
    Inserts
    HHF1
    HTA1
    HTB1
    Cse4
    Tags
    6His-TEV, N-terminal 6His-TEV, and none
    Mutation
    codon-optimized for E. coli -- see provided seque…
    Available Since
    Dec. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    160929-plasmid-map-sequence-id-319094

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  19. ppie.001.301.W257A

    Plasmid
    #137652
    Purpose
    Expresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.
    Depositor
    Tara Davis, Melissa Jurica
    Insert
    ppie (PPIE Human)
    Tags
    GST-(His)6-thrombin
    Expression
    Bacterial
    Mutation
    001-301-W257A
    Available Since
    Jan. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    137652-plasmid-map-sequence-id-381379

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  20. Unc104_K560GFP

    Plasmid
    #129773
    Purpose
    Expresses worm Kinesin-3 Unc104 head and neck linker, dimerized through kinesin-1 neck-coil and coil-1 to 560 and GFP tagged
    Depositor
    William Hancock
    Insert
    Unc104 (unc-104 Nematode)
    Tags
    GFP and His6
    Expression
    Bacterial
    Mutation
    Truncation at 361, fused to kinesin-1 starting Al…
    Available Since
    Nov. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    129773-plasmid-map-sequence-id-263568

    Enlarge

  21. pCJ190

    Plasmid
    #162667
    Purpose
    pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162667-plasmid-map-sequence-id-321443

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  22. pCJ189

    Plasmid
    #162666
    Purpose
    pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by an 8 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by an 8 residue Gly/Ser linker
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available Since
    Feb. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    162666-plasmid-map-sequence-id-321465

    Enlarge

  23. pCJ191

    Plasmid
    #162668
    Purpose
    pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 20 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 20 residue Gly/Ser linker
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162668-plasmid-map-sequence-id-321523

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  24. pCJ202

    Plasmid
    #162675
    Purpose
    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating W159C and S238C mutations.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1)
    Tags
    His
    Expression
    Bacterial
    Mutation
    W159C, S238C; codon optimized for expression in E…
    Promoter
    T7/lac
    Available Since
    Feb. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    162675-plasmid-map-sequence-id-321470

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  25. pCJ206

    Plasmid
    #162679
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating the E226T mutation to the putative lipase box.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), incorporating the E226T mutation to the putative lipase box
    Tags
    His
    Expression
    Bacterial
    Mutation
    E226T; codon optimized for expression in E. coli …
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162679-plasmid-map-sequence-id-321456

    Enlarge

  26. pCJ211

    Plasmid
    #162684
    Purpose
    pET-21b(+) based plasmid for expression of the putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with deletion of the predicted signal peptide (Lys2:Gly19), with C-terminal His tag, codon optimized for expression in E. coli K12.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    Putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) without 19 residue signal peptide
    Tags
    His
    Expression
    Bacterial
    Mutation
    deltaK2:G19; codon optimized for expression in E.…
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162684-plasmid-map-sequence-id-321526

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  27. pCJ197

    Plasmid
    #162670
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S131G mutation.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1
    Tags
    His
    Expression
    Bacterial
    Mutation
    S131G; codon optimized for expression in E. coli …
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162670-plasmid-map-sequence-id-321452

    Enlarge

  28. pCJ205

    Plasmid
    #162676
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating C224A an
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Tags
    His
    Expression
    Bacterial
    Mutation
    C224A, C529A; codon optimized for expression in E…
    Promoter
    T7/lac
    Available Since
    Feb. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    162676-plasmid-map-sequence-id-320828

    Enlarge

  29. pCJ198

    Plasmid
    #162671
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating F495I mutation.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Tags
    His
    Expression
    Bacterial
    Mutation
    F495I; codon optimized for expression in E. coli …
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162671-plasmid-map-sequence-id-321447

    Enlarge

  30. pCJ196

    Plasmid
    #162669
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating catalytic mutation, S225A
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Tags
    His
    Expression
    Bacterial
    Mutation
    S225A; codon optimized for expression in E. coli …
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162669-plasmid-map-sequence-id-321445

    Enlarge

  31. pCJ204

    Plasmid
    #162677
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating C224H and C529F mutations.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Tags
    His
    Expression
    Bacterial
    Mutation
    C224H, C529F; codon optimized for expression in E…
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162677-plasmid-map-sequence-id-321439

    Enlarge

  32. pCJ201

    Plasmid
    #162674
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating C224W and C529SS mutations.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Tags
    His
    Expression
    Bacterial
    Mutation
    C224W, C529SS; codon optimized for expression in …
    Promoter
    T7/lac
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    162674-plasmid-map-sequence-id-320830

    Enlarge

  33. pJK122

    Plasmid
    #74295
    Purpose
    Produces Acetobacter aceti 1023 hypothetical acetic acid resistance protein B, cleavable N-terminal NusA fusion
    Depositor
    T. J. Kappock
    Insert
    hypothetical acetic acid resistance protein B
    Tags
    NusA
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    74295-plasmid-map-sequence-id-141881

    Enlarge

  37. KSP_K406

    Plasmid
    #129771
    Purpose
    Expresses Xenopus Kinesin-5 KSP head and neck linker, dimerized through kinesin-1 neck-coil to 406 for biochemical assays
    Depositor
    William Hancock
    Insert
    KSP (kif11.L Frog)
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Truncation at 367, fused to kinesin-1 starting Al…
    Available Since
    Nov. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    129771-plasmid-map-sequence-id-263572

    Enlarge

Showing: 4561 - 4600 of 15323 results