We narrowed to 13,758 results for: TIM

1. pJY3948

   Plasmid

   #113338

   Purpose

   6XHis-tagged 2-pyrone synthase driven by TEF1intron promoter; codon optimized for Yarrowia lipolytica expression

   Depositor

   Suzanne Sandmeyer

   Article

   Yu et al Biotechnol Bioeng. 2018 May 19. doi: 10.

   Insert

   2-pyrone synthase, codon optimized for Y. lipolytica
   

   Use

   Tags

   6XHis

   Expression

   Yeast

   Mutation

   Please see depositor comments

   Promoter

   Available since

   Oct. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSET FLIPglu-170nDelta13

   Plasmid

   #13562

   Depositor

   Wolf Frommer

   Article

   Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

   Insert

   FLIPglu F16F Delta13
   

   Use

   Tags

   ECFP and EYFP

   Expression

   Bacterial

   Mutation

   WT binding protein (no F16A)

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. KA2058_pX330A-G2P

   Plasmid

   #124202

   Purpose

   Expression of SpCas9 and sgRNA

   Depositor

   Hans Schöler

   Article

   Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEB1-Clover

   Plasmid

   #103991

   Purpose

   Plasmid encoding Clover

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   Clover
   

   Use

   Low copy

   Tags

   Expression

   Bacterial

   Mutation

   Mutations relative to wild-type GFP (S30R, Y39N, …

   Promoter

   proC

   Available since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFH67

   Plasmid

   #131766

   Purpose

   Level1 ZmUBIp::SpCas9 (wheat codon optimized, version 3):NOSt

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   ZmUBIp::SpCas9 (wheat codon optimized, version 3):NOSt
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5/FRT/TO_H2B-HaloTag11-T2A-EGFP

   Plasmid

   #175522

   Purpose

   Nuclear expression of HaloTag11 in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   H2B-HaloTag11
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   HaloTag11 = HaloTag7-M175W

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCIS.MTRIA-CCK

   Plasmid

   #184602

   Purpose

   For expression of a fluorescent sensor for CCK in mammalian cells

   Depositor

   Hiroshi Hibino, Daisuke Ino

   Article

   Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

   Insert

   MTRIA-CCK
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   June 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. BCJJ344

   Plasmid

   #117503

   Purpose

   UBI10:Cas9:E9 Golden Gate Level 1 Position 2 reverse

   Depositor

   Jonathan D Jones

   Article

   Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

   Insert

   BpiI:tagt:UBI10:Cas9-3(intron):E9:ttgc:BpiI
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtUBI10

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFA6a-link-yoClover-SpHis5

   Plasmid

   #44857

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized Clover with SpHis5 selection

   Depositor

   Kurt Thorn, Wendell Lim

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yoClover
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRSET FLIPglu-3.2mDelta13

    Plasmid

    #13560

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

    Insert

    FLIPglu F16A S112A Delta13
    

    Use

    Tags

    ECFP and EYFP

    Expression

    Bacterial

    Mutation

    F16A, S112A in binding protein

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEB1-Venus SX

    Plasmid

    #103990

    Purpose

    Plasmid encoding Venus SX

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    Venus SX
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (F46L, F64L, …

    Promoter

    proC

    Available since

    Feb. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEB1-mVenus JBC

    Plasmid

    #103987

    Purpose

    Plasmid encoding mVenus JBC

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mVenus JBC
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (F46L, F64L, …

    Promoter

    proC

    Available since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. Crimson/P2A-ICP8 (pSLIK1)

    Plasmid

    #113856

    Purpose

    For Doxycycline-inducible expression of ICP8 synaptase, with red fluorescent protein gene E2-Crimson-P2A-ICP8

    Depositor

    Richard S Myers

    Article

    Valledor et al PLoS One. 2018 Aug 15;13(8):e0200955. do

    Insert

    ICP8
    

    Use

    Lentiviral

    Tags

    P2A/Crimson

    Expression

    Mutation

    Promoter

    Available since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. KA1390_pGL4-UBCmin-luc2

    Plasmid

    #124185

    Purpose

    Luciferase reporter with the UBC minimal promoter

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFA6a-link-yomWasabi-SpHis5

    Plasmid

    #44850

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mWasabi with SpHis5 selection

    Depositor

    Kurt Thorn, Wendell Lim

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomWasabi
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRSET FLII275Pglu-4.6m

    Plasmid

    #13565

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

    Insert

    FLIPglu F16A G275S-ECFP-K276R
    

    Use

    Tags

    Citrine

    Expression

    Bacterial

    Mutation

    F16A, ECFP insert in mglB

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEBB-XIAP1-351

    Plasmid

    #11560

    Depositor

    Jon Ashwell

    Article

    Yang et al Science 2000 May 5;288(5467):874-7.

    Insert

    XIAP1-351 (XIAP Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    deleted amino acids 352-496

    Promoter

    Available since

    April 27, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pN_35S/mApple

    Plasmid

    #132566

    Purpose

    mApple expression regulated by the CaMV35S promoter, co-expressed nourseothricin acetyl transferase selectable marker (nat)

    Depositor

    Mathias Pribil

    Article

    Behrendorff et al Physiol Plant. 2020 Feb 25. doi: 10.1111

    Insert

    mApple
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Cauliflower mosaic virus 35S

    Available since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pICSL50016

    Plasmid

    #50315

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    CDS, variant of turboGFP codon-optimised for plants (P. plumata) with intron, U5 small nuclear ribonucleoprotein component (A.
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    Codon-optimised for A. thaliana. BsaI/BbsI sites …

    Promoter

    Available since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. HIV-1 integrase F185H/C280S in pET15b

    Plasmid

    #61672

    Purpose

    HIV-1 integrase F185H/C280S in pET-15b

    Depositor

    Robert Craigie

    Article

    Jenkins et al J Biol Chem. 1996 Mar 29;271(13):7712-8.

    Insert

    HIV-1 integrase F185H/C280S
    

    Use

    Synthetic Biology

    Tags

    His

    Expression

    Mutation

    Promoter

    T7 promotor

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only