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pHW-CIBN-MP-HRW-CRY2olig-VHH(GFP)

Plasmid

#126026

Purpose

express RFP-tagged LARIAT variant for optogenetic clustering

Depositor

Eurico Morais-de-Sá

Article

Osswald et al Biomolecules. 2019 Feb 12;9(2). pii: bio

Insert

CIBN-MP; CRY2 PHRolig-VHH(GFP)

Tags

RFP

Expression

Insect

Mutation

CRY2 E490G

Promoter

Hsp70

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pHW-CIBN-mCerulean-MP-HRW-CRY2-VHH(GFP)

Plasmid

#126025

Purpose

express RFP- and mCer-tagged LARIAT for optogenetic clustering

Depositor

Eurico Morais-de-Sá

Article

Osswald et al Biomolecules. 2019 Feb 12;9(2). pii: bio

Insert

CIBN-mCerulean-MP; CRY2 PHR-VHH(GFP)

Tags

RFP

Expression

Insect

Mutation

WT

Promoter

Hsp70

Available Since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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CKII-NPTX1-pH

Plasmid

#217974

Purpose

Expression of NPTX1-pHluorin for optical monitoring of presynaptic release in neurons

Depositor

Jaime de Juan-Sanz

Article

Pascual-Caro et al PLoS Biol. 2024 Oct 28;22(10):e3002860.

Insert

Neuronal Pentraxin 1-pHluorin

Use

Lentiviral

Tags

pHluorin

Expression

Mammalian

Promoter

CaMKIIa

Available Since

July 9, 2025

Availability

Academic Institutions and Nonprofits only

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CamKII-LGI1-S473L-pHluorin

Plasmid

#185540

Purpose

Expresses mutant S473L LGI1-pHluorin under the CamKII promoter

Depositor

Jaime de Juan-Sanz

Article

Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

Insert

LGI1 (Lgi1 Rat)

Use

Lentiviral

Tags

pHluorin

Expression

Mammalian

Mutation

changed serine 473 for leucine

Promoter

CamKII

Available Since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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CamKII-LGI1-Y433A-pHluorin

Plasmid

#185541

Purpose

Expresses mutant Y433A LGI1-pHluorin under the CamKII promoter

Depositor

Jaime de Juan-Sanz

Article

Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

Insert

LGI1 (Lgi1 Rat)

Use

Lentiviral

Tags

pHluorin

Expression

Mammalian

Mutation

changed tyrosine 433 for alanine

Promoter

CamKII

Available Since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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CamKII-LGI1-R474Q-pHluorin

Plasmid

#185542

Purpose

Expresses mutant R474Q LGI1-pHluorin under the CamKII promoter

Depositor

Jaime de Juan-Sanz

Article

Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

Insert

LGI1 (Lgi1 Rat)

Use

Lentiviral

Tags

pHluorin

Expression

Mammalian

Mutation

changed arginine 474 for glutamine

Promoter

CamKII

Available Since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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CamKII-LGI1-T380A-pHluorin

Plasmid

#185543

Purpose

Expresses mutant T380A LGI1-pHluorin under the CamKII promoter

Depositor

Jaime de Juan-Sanz

Article

Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

Insert

LGI1 (Lgi1 Rat)

Use

Lentiviral

Tags

pHluorin

Expression

Mammalian

Mutation

changed threonine 380 for alanine

Promoter

CamKII

Available Since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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CamKII-LGI1-E383A-pHluorin

Plasmid

#185544

Purpose

Expresses mutant E383A LGI1-pHluorin under the CamKII promoter

Depositor

Jaime de Juan-Sanz

Article

Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

Insert

LGI1 (Lgi1 Rat)

Use

Lentiviral

Tags

pHluorin

Expression

Mammalian

Mutation

changed glutamic acid 383 for alanine

Promoter

CamKII

Available Since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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CamKII-LGI1-C200R-pHluorin

Plasmid

#185545

Purpose

Expresses mutant C200R LGI1-pHluorin under the CamKII promoter

Depositor

Jaime de Juan-Sanz

Article

Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

Insert

LGI1 (Lgi1 Rat)

Use

Lentiviral

Tags

pHluorin

Expression

Mammalian

Mutation

changed cysteine 383 for arginine

Promoter

CamKII

Available Since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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pSF-pMB1'- BAD-YFP

Plasmid

#186567

Purpose

pMB1' ori, L-arabinose PBAD promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression

Depositor

Teresa de Diego Puente

Article

Lozano Terol et al Front Microbiol. 2021 Jun 21;12:682001.

Insert

YFP

Expression

Bacterial

Promoter

BAD promoter

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pSF-p15A-T7- YFP

Plasmid

#186568

Purpose

p15A ori, lacI PT7 promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression

Depositor

Teresa de Diego Puente

Article

Lozano Terol et al Front Microbiol. 2021 Jun 21;12:682001.

Insert

YFP

Expression

Bacterial

Promoter

T7 promoter

Available Since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pSF-pMB1'- trc-YFP

Plasmid

#186565

Purpose

pMB1' ori, lacI, Ptrc promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression

Depositor

Teresa de Diego Puente

Article

Lozano Terol et al Front Microbiol. 2021 Jun 21;12:682001.

Insert

YFP

Expression

Bacterial

Promoter

trc promoter

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pSF-pMB1'- tac-YFP

Plasmid

#186566

Purpose

pMB1' ori, lacI, Ptac promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression

Depositor

Teresa de Diego Puente

Article

Lozano Terol et al Front Microbiol. 2021 Jun 21;12:682001.

Insert

YFP

Expression

Bacterial

Promoter

tac promoter

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pGG-GOI-DE-TurboID-GFP

Plasmid

#209389

Purpose

Golden Gate compatible plasmid for TurboID-based proximity labelling experiment in planta

Depositor

Rosa Lozano-Durán

Article

Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

Type

Empty backbone

Tags

GFP

Expression

Plant

Mutation

TurboID gene sequence C249A

Available Since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pGL2-hOct4 DE-SV40-Luc

Plasmid

#26279

Purpose

Addgene next-generation sequencing identified numerous modifications from the indicated plasmid backbone. Please see the sequences link for additional information.

Depositor

Rudolf Jaenisch

Article

Hanna et al Proc Natl Acad Sci U S A. 2010 May 18. 1

Insert

Oct4 Distal Enhancer Element (DE) (POU5F1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

See Depositor Comments below

Available Since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only

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pDSM-de-Pfba1-PYC-Trpl15a

Plasmid

#127738

Purpose

Yeast pathway position 5. PYC transcription unit with the FBA1 promoter and RPL15A terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

pyruvate carboxylase (PYC1 Synthetic, Budding Yeast)

Use

Synthetic Biology

Expression

Yeast

Promoter

Pfba1

Available Since

Jan. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag BimL(T56A,DE)

Plasmid

#25059

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (T56A,DE) (BCL2L11 Human)

Tags

Flag

Expression

Mammalian

Mutation

Changed threonine 56 to alanine changed leucine …

Available Since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag BimL(T56D,DE)

Plasmid

#25060

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (T56D,DE) (BCL2L11 Human)

Tags

Flag

Expression

Mammalian

Mutation

Changed threonine 56 to aspartic acid changed le…

Available Since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only
pDSM-de-Phhf2-PYC-Tadh1

Plasmid

#127737

Purpose

Yeast pathway position 5. PYC transcription unit with the HHF2 promoter and ADH1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

pyruvate carboxylase (PYC1 Synthetic, Budding Yeast)

Use

Synthetic Biology

Expression

Yeast

Promoter

Phhf2

Available Since

Aug. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3 T7 BimL(S58D,DE)

Plasmid

#25057

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (S58D,DE) (BCL2L11 Human)

Tags

T7

Expression

Mammalian

Mutation

Changed serine 58 to aspartic acid changed leuci…

Available Since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only