We narrowed to 3,800 results for: golden gate

1. LYS-Leu2

   Plasmid

   #218114

   Purpose

   Integration backbone for the LYS2 integration site of S. cerevisiae. Includes a Leu2 nutritional marker and a sfGFP drop-out.

   Depositor

   Carl Denard

   Article

   Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

   Type

   Empty backbone

   Expression

   Bacterial and Yeast

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQ132-tRNA

   Plasmid

   #179211

   Purpose

   Golden Gate entry vector; 2nd gRNA with tRNA

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Promoter

   no promoter

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCHW90004

   Plasmid

   #222436

   Purpose

   Level 0, promoter module (GGAG-CCAT) containing CBS4 and tomato miniDFR promoters.

   Depositor

   Chih-Hang Wu

   Article

   Chiang et al bioRxiv 2024.03.23.586378

   Insert

   CBS4-miniDFR, CBS4 promoter + miniDFR minimal promoter (Solanum lycopersicum)
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   no

   Available Since

   Nov. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCFJ2369 - intron GG1 - 250bp

   Plasmid

   #159877

   Purpose

   Golden Gate compatible intron with PATCs for reduced germline silencing

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   intron GG1 - 250bp
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCHW110033

   Plasmid

   #222441

   Purpose

   Level 0, CDS module (AATG-GCTT) containing Cre recombinase.

   Depositor

   Chih-Hang Wu

   Article

   Chiang et al bioRxiv 2024.03.23.586378

   Insert

   Cre recombinase
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYPQ133D

   Plasmid

   #69289

   Purpose

   Golden Gate entry vector; 1st gRNA under OsU3 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPD100 VP16-ZF43

   Plasmid

   #138761

   Purpose

   Constitutive expression of VP16-ZF43 under the CMV promoter.

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   VP16-ZF43
   

   Use

   Synthetic Biology

   Tags

   3xFlag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCHW110032

   Plasmid

   #222438

   Purpose

   Level 0, promoter module (GGAG-AATG) containing CBS4 and CMV1 promoters.

   Depositor

   Chih-Hang Wu

   Article

   Chiang et al bioRxiv 2024.03.23.586378

   Insert

   CBS4-CMV1, , CBS4 promoter + CMV1 (Cucumber mosaic virus)
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   no

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCHW00011

   Plasmid

   #222439

   Purpose

   Level 0, CDSns module (AATG-TTCG) containing S. cerevisiae CUP2.

   Depositor

   Chih-Hang Wu

   Article

   Chiang et al bioRxiv 2024.03.23.586378

   Insert

   CUP2 (Saccharomyces cerevisiae) (CUP2 Budding Yeast)
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   no

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJJB1561

    Plasmid

    #218626

    Purpose

    Express geraniol synthase (GES), Idi1, and Erg20(F96W, N127W) all with ePTS1 tags and cytosolic Erg20_WT_synthetic homodimer

    Depositor

    John Dueber

    Article

    Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

    Insert

    GES
    

    Tags

    ePTS1

    Expression

    Yeast

    Available Since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPD536 VP16-ZF150

    Plasmid

    #138769

    Purpose

    Constitutive expression of VP16-ZF150 under the CMV promoter.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    VP16-ZF150
    

    Use

    Synthetic Biology

    Tags

    3xFlag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pPD538 VP16-ZF172

    Plasmid

    #138772

    Purpose

    Constitutive expression of VP16-ZF172 under the CMV promoter.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    VP16-ZF172
    

    Use

    Synthetic Biology

    Tags

    3xFlag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. ND4 DdCBE G1

    Plasmid

    #221978

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-T-ND4 TALE G1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. ND4 DdCBE C2

    Plasmid

    #221979

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-T-ND4 TALE C2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. ND4 DdCBE T1

    Plasmid

    #221980

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-T-ND4 TALE T1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. ND4 DdCBE T2

    Plasmid

    #221981

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-T-ND4 TALE T2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ND4 alphaDdCBE G1

    Plasmid

    #221982

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-alphaN-ND4 TALE G1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    TALE: Q231R, W232G, S233A

    Promoter

    CMV

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. ND4 alphaDdCBE C2

    Plasmid

    #221983

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-alphaN-ND4 TALE C2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    TALE: Q231R, W232G, S233A

    Promoter

    CMV

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ND4 alphaDdCBE T1

    Plasmid

    #221984

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-alphaN-ND4 TALE T1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    TALE: Q231R, W232G, S233A

    Promoter

    CMV

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ND4 alphaDdCBE T2

    Plasmid

    #221985

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-alphaN-ND4 TALE T2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    TALE: Q231R, W232G, S233A

    Promoter

    CMV

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge