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We narrowed to 5,211 results for: golden gate

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Showing: 2281 - 2300 of 5211 results

  1. pOPS0381

    Plasmid
    #133231
    Purpose
    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.
    Depositor
    Philip Poole
    Publication
    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117
    Insert
    constructed with constitutive promoter PsNifH (pOGG043), sfGFP (pOGG037) and T-pharma (pOGG003)
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    133231-plasmid-map-sequence-id-282538

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  6. pBFC0993

    Plasmid
    #231993
    Purpose
    CRISPRi-ART plasmid encoding aTc-inducible dRfxCas13d and constitutive crRNA with negative control (RFP-targeting) spacer
    Depositor
    Brady Cress
    Publication
    Adler et al Nat Microbiol. 2025 Mar;10(3):694-709. d
    Inserts
    dRfxCas13d
    crRNA with negative control (RFP-targeting) spacer
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23119 and pTet
    Available since
    April 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    231993-plasmid-map-sequence-id-467616

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  12. pBG-ttLbUV2

    Plasmid
    #231386
    Purpose
    LbCas12a variant with D156R and E795L mutations and harboring same NLS sequence as PEmax but codon-optimized for dicot species; for Agrobacterium-mediated Arabidopsis transformation.
    Depositor
    Qi-Jun Chen
    Publication
    Xin et al aBIOTECH. 2024 Mar 26;5(2):117-126. doi:
    Insert
    ttLbCas12a Ultra V2
    Use
    CRISPR
    Expression
    Plant
    Mutation
    D156R and E795L
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    231386-plasmid-map-sequence-id-461803

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  19. pBFC1171

    Plasmid
    #231994
    Purpose
    CRISPRi-ART crRNA only plasmid encoding crystal violet-inducible crRNA (for dRfxCas13d) with 2xBsaI spacer cloning site
    Depositor
    Brady Cress
    Publication
    Adler et al Nat Microbiol. 2025 Mar;10(3):694-709. d
    Insert
    crRNA of dRfxCas13d
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    pJEx (Jungle Express)
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    231994-plasmid-map-sequence-id-468506

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Showing: 2281 - 2300 of 5211 results