165,947 results

1. pLV[Exp]-Puro-CMV>{ARL13B_6gly_GFP_6Gly_R_GECO1}

   Plasmid

   #232880

   Purpose

   Expresses the ARL13B coupled to GFP and RGECO1 calcium sensor.

   Depositor

   Paul DeCaen

   Article

   DeCaen et al J Cell Physiol. 2025 Apr;240(4):e70034.

   Insert

   ADP-ribosylation factor-like protein 13B (ARL13B Human)
   

   Use

   Lentiviral

   Tags

   GFP and RGECO1

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. gTBEv4

   Plasmid

   #220620

   Purpose

   vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv4) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

   Depositor

   Huawei Tong

   Article

   Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:

   Insert

   nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
   

   Expression

   Mammalian

   Mutation

   hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…

   Promoter

   hU6, EF1a

   Available Since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBMN mCherry-OPTN(D474N)

   Plasmid

   #119683

   Purpose

   For mammalian expression of UBD mutant OPTN

   Depositor

   Michael Lazarou

   Article

   Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

   Insert

   OPTN (OPTN Human)
   

   Use

   Retroviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   D474N

   Available Since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLJM1 EGFP-Tau

   Plasmid

   #108868

   Purpose

   Expresses EGFP linked to human Tau

   Depositor

   Gabriele Kaminski Schierle

   Insert

   Tau (MAPT Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   human four-repeat tau lacking the N-terminal sequ…

   Promoter

   CMV

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. PDG459 V3

   Plasmid

   #226958

   Purpose

   CBh-SpCas9-2A-Puro, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. LentiGuide Cherry

   Plasmid

   #170510

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and mCherry marker from EF-1a promoter. 3rd generation lentiviral backbone.

   Depositor

   Pavel Tolar

   Article

   Newman et al biorXiv 2021.03.02.433398

   Inserts

   S. pyogenes sgRNA cassette
   mCherry
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1a and hU6

   Available Since

   Sept. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR223_DKK1_WT

   Plasmid

   #82250

   Purpose

   Gateway Donor vector containing DKK1 , part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, William Hahn, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   DKK1 (DKK1 Human)
   

   Use

   Gateway entry vector

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pU6-GG-gRNA

   Plasmid

   #226860

   Purpose

   Human gRNA expression vector backbone with BsmBI Golden Gate site

   Depositor

   Alexis Komor

   Article

   Osgood et al Methods in Enzymology, February 5, 2025

   Insert

   Sp-gRNA
   

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-INSR-V5/HIS

   Plasmid

   #201980

   Purpose

   expression of human INSR receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   INSR receptor tyrosine kinase (INSR Human)
   

   Tags

   V5, 6xHis

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV5 HA-TBRII (delta Cyt)

    Plasmid

    #14051

    Depositor

    Joan Massague

    Article

    Siegel et al Proc Natl Acad Sci U S A. 2003 Jul 8. 10

    Insert

    TGF beta rec II (delta cyt) (TGFBR2 Human)
    

    Tags

    HA (PYDVPDYA)

    Expression

    Mammalian

    Mutation

    Dominant negative mutant. A stop codon was introd…

    Available Since

    May 10, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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