We narrowed to 9,317 results for: bli

1. pIND4-RC-C2-MmXYRS

   Plasmid

   #222744

   Purpose

   Expresses 3 subunits of photosynthetic reaction center from R. sphaeroides and aaRS-tRNA pair for incorporating haloalkane crosslinker

   Depositor

   Steven Boxer

   Inserts

   puhA
   pufL
   pufM
   MmXYRS
   Mm Pyl tRNA
   

   Tags

   6xHis

   Mutation

   Contains the following substitutions: V346A, W348…

   Promoter

   rrnB

   Available Since

   Sept. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. AiP1999 - pAAV-AiE2255m-minBG-iCre(R297T)-BGHpA

   Plasmid

   #223843

   Purpose

   Enhancer-driven expression of the iCre(R297T) recombinase in thalamic PVT neurons

   Depositor

   Bosiljka Tasic

   Insert

   iCre(R297T)
   

   Use

   AAV

   Mutation

   Argenine in aa297 was replaced with Threonine

   Promoter

   minBG

   Available Since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBV90

   Plasmid

   #204601

   Purpose

   Expresses OsTir1-3X Myc from S. cerevisiae ADH1 promoter

   Depositor

   Scott Moye-Rowley

   Insert

   Os Tir1 (LOC4338955 Oryza sativa)
   

   Tags

   Myc epitope tags

   Expression

   Bacterial

   Promoter

   ScADH1

   Available Since

   July 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYES2/NTA/Leu-LegA7

   Plasmid

   #216518

   Purpose

   Expression of LegA7

   Depositor

   Ralph Isberg

   Insert

   Leg A7
   

   Tags

   HIS-tag

   Expression

   Bacterial and Yeast

   Mutation

   LegA7 WT

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pet15b _yein

   Plasmid

   #220226

   Purpose

   Bacterial expression of YeiN in E. coli BL21_DE3 (pLys) or similar

   Depositor

   Bernd Nidetzky

   Insert

   psug (psuG E.coli)
   

   Tags

   Histag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-29b(+)_notufA-CmEndoCOM_3C-CBD-His

   Plasmid

   #218198

   Purpose

   Bacterial expression of a His-tagged Chitin Binding fusion of Cordyceps militaris EndoCOM

   Depositor

   Florence Pojer

   Insert

   EndoCOM
   

   Tags

   3C-CBD-His

   Expression

   Bacterial

   Mutation

   codon optimized Ecoli, residues 19-315

   Promoter

   T7

   Available Since

   April 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. m.3047-Left TALE-G1397-N-dead-mCherry

   Plasmid

   #211339

   Purpose

   Expression of codon-optimized dead mitochondrial base editor in human cells; cell sorting; blasticidin drug selection

   Depositor

   Monkol Lek

   Insert

   m.3047-Left TALE-G1397-N-dead-mCherry
   

   Use

   TALEN

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. m.3075-Left TALE-G1397-N-dead-mCherry

   Plasmid

   #211340

   Purpose

   Expression of codon-optimized dead mitochondrial base editor in human cells; cell sorting; blasticidin drug selection

   Depositor

   Monkol Lek

   Insert

   m.3075-Left TALE-G1397-N-dead-mCherry
   

   Use

   TALEN

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. m.5147-Left TALE-G1397-N-dead-mCherry

   Plasmid

   #211341

   Purpose

   Expression of codon-optimized dead mitochondrial base editor in human cells; cell sorting; blasticidin drug selection

   Depositor

   Monkol Lek

   Insert

   m.5147-Left TALE-G1397-N-dead-mCherry
   

   Use

   TALEN

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. m.3075-Right Puro DdCBE-G1397-C- T1413I-GFP

    Plasmid

    #211343

    Purpose

    Expression of codon-optimized mitochondrial base editor in human cells; cell sorting; puromycin drug selection

    Depositor

    Monkol Lek

    Insert

    m.3075-Right Puro DdCBE-G1397-C- T1413I-GFP
    

    Use

    TALEN

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-CAG-K-SPOTIT(R271F)

    Plasmid

    #191492

    Purpose

    Green fluorescent-based opioid sensor for the kappa opioid receptor with reduced Gai coupling in AAV viral vector under a CAG promoter

    Depositor

    Wenjing Wang

    Insert

    K-SPOTIT(R271F)
    

    Use

    AAV

    Mutation

    R271F in OR

    Promoter

    CAG

    Available Since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV-CAG-M-SPOTIT2(R279F)

    Plasmid

    #191493

    Purpose

    Green fluorescent-based opioid sensor for the mu opioid receptor with reduced Gai coupling in AAV viral vector under a CAG promoter

    Depositor

    Wenjing Wang

    Insert

    M-SPOTIT2(R279F)
    

    Use

    AAV

    Mutation

    R279F in OR

    Promoter

    CAG

    Available Since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. PAS_HeSAHS4

    Plasmid

    #215648

    Purpose

    Expresses SUMO-tagged HeSAHS4 protein from tardigrade H. exemplaris in E. coli

    Depositor

    Pamela Silver

    Insert

    SAHS4
    

    Tags

    SUMO

    Expression

    Bacterial

    Available Since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. PAS_RvSAHS4

    Plasmid

    #215646

    Purpose

    Expresses SUMO-tagged RvSAHS4 protein from tardigrade R. varieornatus in E. coli

    Depositor

    Pamela Silver

    Insert

    SAHS4
    

    Tags

    SUMO

    Expression

    Bacterial

    Available Since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX458-tRNA-SpCas9

    Plasmid

    #195183

    Purpose

    Vector for transient Cas9 and EGFP expression. Small tRNA promoter for sgRNA cloning by GoldenGate.

    Depositor

    Stephanie Roessler

    Insert

    Cas9
    

    Use

    CRISPR

    Promoter

    tRNA-Gln

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pT7CFE1_EVD68_VP12A_C107R

    Plasmid

    #212982

    Purpose

    Encodes EV-D68 MO/14/18947 VP12A with active site mutation optimized for expression in mammalian transcription/translation extracts

    Depositor

    Karla Kirkegaard

    Insert

    EV-D68 MO/14/18947 VP12A C107R
    

    Use

    Mammalian cell free protein expression

    Tags

    HA

    Mutation

    changed cysteine 107 from 2A to Arginine

    Promoter

    T7

    Available Since

    Feb. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pT7CFE1_EVD68_3ABC_C147R

    Plasmid

    #212978

    Purpose

    Encodes EV-D68 MO/14/18947 3ABC with active site mutation optimized for expression in mammalian transcription/translation extracts

    Depositor

    Karla Kirkegaard

    Insert

    EV-D68 MO/14/18947 3ABC C147R
    

    Use

    Mammalian cell free protein expression

    Tags

    HA

    Mutation

    changed Cysteine 147 in 3C to Arginine

    Promoter

    T7

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pT7CFE1_EVD68_3ABCD_C147R

    Plasmid

    #212980

    Purpose

    Encodes EV-D68 MO/14/18947 3ABCD with active site mutation optimized for expression in mammalian transcription/translation extracts

    Depositor

    Karla Kirkegaard

    Insert

    EV-D68 MO/14/18947 3ABCD C147R
    

    Use

    Mammalian cell free protein expression

    Tags

    HA

    Mutation

    changed cysteine 147 from 3C to arginine

    Promoter

    T7

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pT7CFE1_EVD68_VP12A_N84T

    Plasmid

    #212983

    Purpose

    Encodes EV-D68 MO/14/18947 VP12A with telaprevir resistance mutation optimized for expression in mammalian transcription/translation extracts

    Depositor

    Karla Kirkegaard

    Insert

    EV-D68 MO/14/18947 VP12A N84T
    

    Use

    Mammalian cell free protein expression

    Tags

    HA

    Mutation

    changed Asparagine 84 from 2A to Threonine

    Promoter

    T7

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28-Flag-RBM24-1~93

    Plasmid

    #208633

    Purpose

    for E. coli expression of truncated Flag tagged human RBM24 (ENST00000379052), aa1~93

    Depositor

    Quanfu Ma

    Insert

    human RBM24 (ENST00000216146) aa 1~93 (RBM24 Human)
    

    Tags

    6xHis and Flag

    Expression

    Bacterial

    Mutation

    only aa 1~93, other residues not included

    Promoter

    T7

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28-RPL3-Twstrep

    Plasmid

    #208623

    Purpose

    for E. coli expression of C-ter Twin Strep tagged human RPL3 (ENST00000216146)

    Depositor

    Quanfu Ma

    Insert

    human RPL3 (ENST00000216146) (RPL3 Human)
    

    Tags

    6xHis and Twin Strep

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAMW-Wnk

    Plasmid

    #203978

    Purpose

    Myc6 Wnk under actin promoter for cell culture

    Depositor

    Andreas Jenny

    Insert

    Wnk (Wnk Fly)
    

    Tags

    6xMyc

    Expression

    Insect

    Available Since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. XBB.1.5+R403K

    Plasmid

    #208586

    Purpose

    Mammalian expression vector for expressing XBB.1.5+R403K SARS-CoV-2 Spike, with 19AA cytoplasmic tail truncation

    Depositor

    Ben Murrell

    Insert

    SARS-CoV-2 variant XBB.1.5+R403K spike (S )
    

    Expression

    Mammalian

    Available Since

    Oct. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. XBB.1.5+F456L

    Plasmid

    #208585

    Purpose

    Mammalian expression vector for expressing XBB.1.5+F456L SARS-CoV-2 Spike, with 19AA cytoplasmic tail truncation

    Depositor

    Ben Murrell

    Insert

    SARS-CoV-2 variant XBB.1.5+F456L spike (S )
    

    Expression

    Mammalian

    Available Since

    Oct. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. XBB.1.5+K478R

    Plasmid

    #208587

    Purpose

    Mammalian expression vector for expressing XBB.1.5+K478R SARS-CoV-2 Spike, with 19AA cytoplasmic tail truncation

    Depositor

    Ben Murrell

    Insert

    SARS-CoV-2 variant XBB.1.5+K478R spike (S )
    

    Expression

    Mammalian

    Available Since

    Oct. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCDH-Flag-hS1pr1-S5A_Neo

    Plasmid

    #203710

    Purpose

    Overexpression of FLAG-S1PR1-S5A mutant.

    Depositor

    Timothy Hla

    Insert

    Flag-hS1PR1-S5A (S1PR1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S5A

    Promoter

    CMV

    Available Since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-hPhyB621(Y276H)-P2A-m1V

    Plasmid

    #197377

    Purpose

    The AAV vector for PhyB621(Y276H) and m1V

    Depositor

    Michiyuki Matsuda

    Insert

    Phytochrome B Y276H(1-621 aa)
    

    Use

    AAV

    Tags

    P2A-m1V

    Mutation

    Human codon optimized PhyB (1-621 aa), Y276H muta…

    Promoter

    CMV

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pALuWBB

    Plasmid

    #183202

    Purpose

    dsRed marked transgene for Crispr co-conversion with w+ gRNA and drop in cloning sites for 2 more gRNA

    Depositor

    Mitch McVey

    Insert

    w+ gRNA and scaffold followed by 2 gRNA with BbsI and BsaI cloning sites
    

    Use

    CRISPR; Recombines at attp landing site in fly ge…

    Expression

    Insect

    Promoter

    U6-3

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. PVALB mAb_BCN14.1.1A2_HC_Rat IgG2a

    Plasmid

    #182894

    Purpose

    Mammalian expression of PVALB rat mAb HC gene

    Depositor

    Heng Zhu

    Insert

    anti-PVALB (human) heavy chain (PVALB Rat)
    

    Use

    Affinity Reagent/ Antibody

    Expression

    Mammalian

    Mutation

    No

    Promoter

    CMV

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. PVALB mAb_BCN14.1.1A2_LC_Rat Ig Kappa

    Plasmid

    #182895

    Purpose

    Mammalian expression of PVALB rat mAb LC gene

    Depositor

    Heng Zhu

    Insert

    anti-PVALB (human) light chain (PVALB Rat)
    

    Use

    Affinity Reagent/ Antibody

    Expression

    Mammalian

    Mutation

    No

    Promoter

    CMV

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. TRE3GV-somBiPOLES-mKate2

    Plasmid

    #192579

    Purpose

    AAV-vector for bidirectional optogenetic manipulation of tTA-positive neurons

    Depositor

    Simon Wiegert

    Insert

    somBiPOLES
    

    Use

    AAV

    Tags

    soma-targeting motif from Kv2.1 channel

    Expression

    Mammalian

    Promoter

    TRE3GV

    Available Since

    Dec. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. TRE3GV-somBiPOLES-mCerulean

    Plasmid

    #192578

    Purpose

    AAV-vector for bidirectional optogenetic manipulation of tetTag-positive neurons

    Depositor

    Simon Wiegert

    Insert

    somBiPOLES
    

    Use

    AAV

    Tags

    soma-targeting motif from Kv2.1 channel

    Expression

    Mammalian

    Promoter

    TRE3GV

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. CaMKII-somBiPOLES-eGFP

    Plasmid

    #192583

    Purpose

    AAV-vector for bidirectional optogenetic manipulation of excitatory neurons

    Depositor

    Simon Wiegert

    Insert

    somBiPOLES
    

    Use

    AAV

    Tags

    soma-targeting motif from Kv2.1 channel

    Expression

    Mammalian

    Promoter

    CaMKIIa(0.4)

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLenti-PGK-NXPH4 (neo)

    Plasmid

    #192078

    Purpose

    Lentivirus for expression of NXPH4

    Depositor

    Roland Friedel

    Insert

    NXPH4 (NXPH4 Human)
    

    Use

    Lentiviral

    Promoter

    hPGK

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pSpCas9_mouse_Adar1_ccC

    Plasmid

    #158121

    Purpose

    deletion mAdar1

    Depositor

    Emanuela Felley-Bosco

    Insert

    Adar1 (Adar Mouse)
    

    Expression

    Mammalian

    Available Since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET-15b(+) Toxoplasma gondii (delta prenylation motif)

    Plasmid

    #170789

    Purpose

    Expresses pET-15b(+) Toxoplasma gondii (delta prenylation motif)

    Depositor

    Kalle Gehring

    Insert

    Toxoplasma gondii PRL
    

    Tags

    N-terminal His-tag

    Expression

    Bacterial

    Mutation

    Deleted nucleotides coding for 4 C-terminal amino…

    Promoter

    AmpR, lacI

    Available Since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pB6-Rosa26-cre_dre_reporter_1_2

    Plasmid

    #185966

    Purpose

    B6J Rosa26-cre dre reporter 1.2 allele targeting vector, low copy number

    Depositor

    Mario Capecchi

    Insert

    sfGFP, tdTomato
    

    Use

    Cre/Lox and Mouse Targeting; Dre/rox

    Expression

    Mammalian

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pB6-Rosa26-cre_dre_reporter_1_1b

    Plasmid

    #185965

    Purpose

    B6J Rosa26-cre dre reporter 1.1b allele targeting vector, low copy number

    Depositor

    Mario Capecchi

    Insert

    sfGFP, tdTomato
    

    Use

    Cre/Lox and Mouse Targeting; Dre/rox

    Expression

    Mammalian

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pB6-Rosa26-cre_dre_reporter_1_1a

    Plasmid

    #185964

    Purpose

    B6J Rosa26-cre dre reporter 1.1a allele targeting vector, low copy number

    Depositor

    Mario Capecchi

    Insert

    sfGFP, tdTomato
    

    Use

    Cre/Lox and Mouse Targeting; Dre/rox

    Expression

    Mammalian

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pB6-Rosa26-cre_dre_reporter_1_1

    Plasmid

    #185963

    Purpose

    B6J Rosa26-cre dre reporter 1.1 allele targeting vector, low copy number

    Depositor

    Mario Capecchi

    Insert

    EGFP, tdTomato
    

    Use

    Cre/Lox and Mouse Targeting; Dre/rox

    Expression

    Mammalian

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge