We narrowed to 31,918 results for: ica
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Plasmid#240451PurposeExpression of indicator protein fusion (modified ratiometric pHluorin & low affinity Aequorin) for monitoring calcium concentrations and pH in cell walls of higher plants. See Resource Information.DepositorInsertFusion of chitinase signal, soluble modified ratiometric pHluorin and low affinity Aequorin (D119A)
ExpressionBacterial and PlantMutationRatiometric GFP (ratiometric pHluorin) (PMID 9671…PromoterCauliflower mosaic virus 35S promoter (GenBank AJ…Available SinceSept. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLCKO-CMV-Blasticidin-P2A-3xHA-TMEM106B_Monkey
Plasmid#238132PurposeThis plasmid encodes the full-length African Green Monkey (Chlorocebus sabaeus) TMEM106B protein fused with a 3xHA tag, along with an N-terminal blasticidin selection marker.DepositorInsertTMEM106B
UseLentiviralTagsBlasticidin-P2A-3xHAExpressionMammalianPromoterCMVAvailable SinceJune 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyso-InPTapp
Plasmid#236000PurposeGenetically encoded FRET-based phosphoinositide sensor for monitoring PI(3,4)P2. Targeted to lysosome surface.DepositorTagsCitrine, Full-length LAMP1, and truncECFPExpressionMammalianPromoterCMVAvailable SinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST (I224A/L227A; deltaLIR3)
Plasmid#223775PurposeExpression of recombinant protein for purificationDepositorInsertBCL2L13 soluble part (BCL2L13 Human)
TagsEGFP-TEV-GSTExpressionBacterialMutationI224A/L227A; deletion of LIR3Available SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST (I307A/V310A; deltaLIR4)
Plasmid#223776PurposeExpression of recombinant protein for purificationDepositorInsertBCL2L13 soluble part (BCL2L13 Human)
TagsEGFP-TEV-GSTExpressionBacterialMutationI307A/V310A; deletion of LIR4Available SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-Dual_BNIP3(1-158aa)-THROMBIN-GST (E44A L47A D49A A50K Q51A; deltaWIPI2)
Plasmid#223777PurposeExpression of recombinant protein for purificationDepositorInsertBNIP3 soluble part (BNIP3 Human)
TagsThrombin cleavage-GSTExpressionInsectMutationE44A L47A D49A A50K Q51A; disrupts WIPI2 bindingAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T1_GST-EGFP-ATG13_IDR_191-517aa_(M196-H214 11xA)
Plasmid#223779PurposeExpression of recombinant protein for purificationDepositorInsertATG13 IDR (ATG13 Human)
TagsGST-EGFPExpressionBacterialMutationexpresses aa 191-517, with M196A S197A R199A G202…Available SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST (I224A/L227A/W276A/I279A; deltaLIR1+3)
Plasmid#223780PurposeExpression of recombinant protein for purificationDepositorInsertBCL2L13 soluble part (BCL2L13 Human)
TagsEGFP-TEV-GSTExpressionBacterialMutationI224A/L227A/W276A/I279A; deletion of LIR1+3Available SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST (W276A/I279A/I307A/V310A; deltaLIR1+4)
Plasmid#223781PurposeExpression of recombinant protein for purificationDepositorInsertBCL2L13 soluble part (BCL2L13 Human)
TagsEGFP-TEV-GSTExpressionBacterialMutationW276A/I279A/I307A/V310A; deletion of LIR1+4Available SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST (Y213A/I216A/W276A/I279A; deltaLIR1+2)
Plasmid#223782PurposeExpression of recombinant protein for purificationDepositorInsertBCL2L13 soluble part (BCL2L13 Human)
TagsEGFP-TEV-GSTExpressionBacterialMutationY213A/I216A/W276A/I279A; deletion of LIR1+2Available SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST (Y213A/I216A; deltaLIR2)
Plasmid#223783PurposeExpression of recombinant protein for purificationDepositorInsertBCL2L13 soluble part (BCL2L13 Human)
TagsEGFP-TEV-GSTExpressionBacterialMutationY213A/I216A; deletion of LIR2Available SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST (I224A/L227A/W276A/I279A/I307A/V310A; deltaLIR1+3+4)
Plasmid#223784PurposeExpression of recombinant protein for purificationDepositorInsertBCL2L13 soluble part (BCL2L13 Human)
TagsEGFP-TEV-GSTExpressionBacterialMutationI224A/L227A/W276A/I279A/I307A/V310A; deletion of …Available SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_BCL2L13(1-465aa)-THROMBIN-GST (I224A/L227A/W276A/I279A; deltaLIR1+3)
Plasmid#223754PurposeExpression of recombinant protein for purificationDepositorInsertBCL2L13 soluble part (BCL2L13 Human)
TagsThrombin cleavage-GSTExpressionBacterialMutationexpresses aa 1-465, with I224A/L227A/W276A/I279A;…Available SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_BCL2L13(1-465aa)-THROMBIN-GST (W276A/I279A/I307A/V310A; deltaLIR1+4)
Plasmid#223755PurposeExpression of recombinant protein for purificationDepositorInsertBCL2L13 soluble part (BCL2L13 Human)
TagsThrombin cleavage-GSTExpressionBacterialMutationexpresses aa 1-465, with W276A/I279A/I307A/V310A;…Available SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_BCL2L13(1-465aa)-THROMBIN-GST (I224A/L227A/W276A/I279A/I307A/V310A; deltaLIR1+3+4)
Plasmid#223756PurposeExpression of recombinant protein for purificationDepositorInsertBCL2L13 soluble part (BCL2L13 Human)
TagsThrombin cleavage-GSTExpressionBacterialMutationexpresses aa 1-465, with I224A/L227A/W276A/I279A/…Available SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T1_GST-TEV-EGFP-ATG13_IDR_191-517aa_(M196A_S197A_R199A)
Plasmid#223761PurposeExpression of recombinant protein for purificationDepositorInsertATG13 IDR (ATG13 Human)
TagsGST-TEV-EGFPExpressionBacterialMutationexpresses aa 191-517, with M196A S197A R199A (par…Available SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_FUNDC1(1-50aa)-THROMBIN-GST (Y18A/L21A; deltaLIR)
Plasmid#223739PurposeExpression of recombinant protein for purificationDepositorInsertFUNDC1 soluble part, mutant (FUNDC1 Human)
TagsThrombin cleavage-GSTExpressionBacterialMutationY18A/L21A; deletion of LIRAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST (W276A/I279A; deltaLIR1)
Plasmid#223746PurposeExpression of recombinant protein for purificationDepositorInsertBCL2L13 soluble part (BCL2L13 Human)
TagsEGFP-TEV-GSTExpressionBacterialMutationW276A/I279A; deletion of LIR1Available SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_NIX(1-182aa)-EGFP-TEV-GST (W36A/L39A; deltaLIR)
Plasmid#223748PurposeExpression of recombinant protein for purificationDepositorInsertNIX (BNIP3L) soluble part (BNIP3L Human)
TagsEGFP-TEV-GSTExpressionBacterialMutationW36A/L39A; deletion of LIRAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_BCL2L13(1-465aa)-THROMBIN-GST (W276A/I279A; deltaLIR1)
Plasmid#223749PurposeExpression of recombinant protein for purificationDepositorInsertBCL2L13 soluble part (BCL2L13 Human)
TagsThrombin cleavage-GSTExpressionBacterialMutationexpresses aa 1-465, with W276A/I279A; deletion of…Available SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_FUNDC1(1-50aa)-EGFP-TEV-GST (Y18A/L21A; deltaLIR)
Plasmid#223750PurposeExpression of recombinant protein for purificationDepositorInsertFUNDC1 soluble part (FUNDC1 Human)
TagsEGFP-TEV-GSTExpressionBacterialMutationexpresses aa 1-50, with Y18A/L21A; deletion of LIRAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_BCL2L13(1-465aa)-THROMBIN-GST (Y213A/I216A/W276A/I279A; deltaLIR1+2)
Plasmid#223752PurposeExpression of recombinant protein for purificationDepositorInsertBCL2L13 soluble part (BCL2L13 Human)
TagsThrombin cleavage-GSTExpressionBacterialMutationexpresses aa 1-465, with Y213A/I216A/W276A/I279A;…Available SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_NIX(1-182aa)-THROMBIN-GST (E72A/L75A/D77A/E81A; deltaWIPI2)
Plasmid#223753PurposeExpression of recombinant protein for purificationDepositorInsertNIX (BNIP3L) soluble part (BNIP3L Human)
TagsThrombin cleavage-GSTExpressionBacterialMutationexpresses aa 1-182, with E72A/L75A/D77A/E81A; del…Available SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn T21R(I18R+M72E)-F8.2(S54L+T127A)-T2A-mCherry
Plasmid#225591PurposeAAV transgene plasmid with hSyn promoter for expression of catalytically dead TRIM21 RING(I18R+M72E)-F8.2(S54L+T127A) anti-tau degrader with a self-cleaving T2A-mCherry fluorescent expression reporterDepositorInsertT21R(I18R+M72E)-F8.2(S54L+T127A)-T2A-mCherry
UseAAVTagsmCherryExpressionMammalianPromoterhSynAvailable SinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IP-muGFP-hATG2A mLIRΔWIR
Plasmid#215508PurposeExpresses GFP tagged human ATG2A which has the mutation in LC3 interacting region and lacks WIPI interacting region.DepositorInsertAutophagy related 2A (ATG2A Human)
UseRetroviralTagsmuGFPExpressionMammalianMutationP656R (natural variant with no functional relevan…Available SinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV2ss-EFS-GFP-synPolyA-U6-sgHTT1-U6-sgCas9
Plasmid#190903PurposeAAV-KamiCas9 vector expressing expressing thew GFP reporter gene and sgHTT and sgCas9DepositorAvailable SinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIGF1_P2 Promoter-FLuc2P
Plasmid#154262PurposeDestabilized firefly luciferase reporter containing the downstream Human IGF-1 P2 Promoter.DepositorAvailable SinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
His6-TEV-CRBN-midi
Plasmid#215330PurposeCRBN midi construct for use in crystallography and biophysical studies enabling ligand and degrader designDepositorInsertCRBN (CRBN Human)
Tags6xHis and TEVExpressionBacterialMutationcontains amino acids 41-187 followed by a Gly-Ser…PromoterlacIAvailable SinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ICUE4
Plasmid#181846PurposeFourth-generation FRET-based Indicator of cAMP Using Epac. Genetically encoded cAMP indicator. Contains high-sensitivity Epac Q270E mutation.DepositorInsertICUE4 (RAPGEF3 Human)
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationGlutamine 122 in Epac1 domain mutated to glutamat…PromoterCMVAvailable SinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
GST-GGA3-VHS-GAT
Plasmid#173719PurposeVHS-GAT domain in GGA3 can bind to an active form of ARF1 protein. Using this construct, you can assess the activity of ARF1 protein.DepositorAvailable SinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAtVPZv1.0
Plasmid#233147PurposeExpresses VDE, PsbS, ZEP from A.thalianaDepositorInsertsExpressionPlantPromoterFBA-2, GAPA-1, Rbcs1a, and mas promoterAvailable SinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-SLC-His+MFN2[∆TM,∆HB2]-Strep (SB261)
Plasmid#227610PurposeInducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 lacking its transmembrane region and helical bundle 2 (HB2) in Pichia pastorisDepositorTags10xHis and Twin-StrepTagExpressionYeastMutationcontains aa 2-400, 706-757 [TM and HB2 deleted]PromoterAOX1Available SinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLT3REVIR_MARK3 2573
Plasmid#107233PurposeshRNA 2573. Do not use Evos or Acurri on these cells. Assess by FACS specifically with Venus and DsRed lazers. Tet-ON-all-in-oneDepositorAvailable SinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLT3REVIR_MARK3 1122
Plasmid#107232PurposeshRNA 1122. Do not use Evos or Acurri on these cells. Assess by FACS specifically with Venus and DsRed lazers. Tet-ON-all-in-oneDepositorAvailable SinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGT3
Plasmid#15825DepositorTypeEmpty backboneExpressionBacterialAvailable SinceOct. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
AiP14908 - pAAV-AiE0354h-minBG-mScarlet-WPRE3-BGHpA (Alias: CN4908)
Plasmid#224052PurposeAiE0354h is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Vip+ neuronsDepositorInsertmScarlet
UseAAVMutationNAPromoterminBGAvailable SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
AiP11969 - pAAV-hsA2-AiE0140h-minRho-SYFP2-4X2C-WPRE-HGHpA (Alias: CN1969)
Plasmid#223969PurposeAiE0140h is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Pvalb+ neuronsDepositorInsertSYFP2
UseAAVMutationNAPromoterminBGAvailable SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
AiP15262 - pAAV-AiE0219h-minBG-mScarlet-WPRE3-BGHpA (Alias: CN5262)
Plasmid#224062PurposeAiE0219h is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Vip+ neuronsDepositorInsertmScarlet
UseAAVMutationNAPromoterminBGAvailable SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
AiP12546 - pAAV-AiE0050m_3xC2-minBG-tdTomato-WPRE3-BGHpA (Alias: CN2546)
Plasmid#224060PurposeAiE0050m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L4/5_IT neuronsDepositorInserttdTomato
UseAAVMutationNAPromoterminBGAvailable SinceFeb. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
AiP12293 - pAAV-3xSP10ins-AiE0219h_3xC2-minRho-SYFP2-8X2C-WPRE3-BGHpA (Alias: CN2293)
Plasmid#223980PurposeAiE0219h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Vip+ neuronsDepositorInsertSYFP2
UseAAVMutationNAPromoterminBGAvailable SinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only