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Addgene

We narrowed to 34,443 results for: CaS

Showing: 5751 - 5800 of 34443 results
  1. pDrop

    Plasmid
    #184872
    Purpose
    Chromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for in-vivo excision of transferred DNA for homologous recombination and counter-selection for successful integration.
    Depositor
    Insert
    lacZalpha,ccdB
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 3, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
  2. pJEC581

    Plasmid
    #174369
    Purpose
    RFP reporter used to evaluate CRISPRa in bacteria, contains gRNA target sites every 10 bp upstream of the promoter.
    Depositor
    Insert
    RFP with PAM rich sequence upstream promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23117
    Available since
    Oct. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
  3. pEPOR1CB0113

    Plasmid
    #117552
    Purpose
    Level 1 Golden Gate Cassette: eSaCas9 expression cassette for dicotyledonous plants
    Depositor
    Insert
    2x35S+5'UTR OMEGA (pICH51288) + eSaCas9 (no stop codon) (pEPOR0SP0012) + YFP (pICSL50005) + Nos (pICH41421)
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    2x35S+5'UTR OMEGA (pICH51288)
    Available since
    Nov. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
  4. pEPOR2KN0079

    Plasmid
    #117646
    Purpose
    Level2 MoClo construct, containing level1 plant expression cassettes for SaCas9(pEPOR1CB0015) and sgRNA_ENTH/ANT {AT1G14910}(pEPOR1CB0100)and sgRNA_ENTH/ANT {AT2G01600}(pEPOR1CB0111)
    Depositor
    Insert
    [35S:SaCas9(pEPOR1CB0015) ] +[AtU6-26:sgRNA]
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Nov. 16, 2018
    Availability
    Academic Institutions and Nonprofits only
  5. Tol2-U6.3-sgRNA-non-targeting-control -GFP

    Plasmid
    #221844
    Purpose
    Tol2 transposon expresses control non-targeting sgRNA from chick U6.3 promoter expresses GFP reporter from GAGC promoter
    Depositor
    Inserts
    EGFP
    non-targeting control sgRNA- GCACTGCTACGATCTACACC
    Use
    CRISPR; Tol2 transposon optimised for chick expre…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    GACG and U6.3 chick
    Available since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
  6. pEPOR2KN0055

    Plasmid
    #117622
    Purpose
    Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.1(pEPOR1CB0011) and sgRNA_Methyltransferase {AT5G10620}(pEPOR1CB0083)
    Depositor
    Insert
    [35S:Sp-eCas9 1.1(pEPOR1CB0011) ] +[AtU6-26:sgRNA]
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    June 4, 2019
    Availability
    Academic Institutions and Nonprofits only
  7. pEPOR2KN0078

    Plasmid
    #117645
    Purpose
    Level2 MoClo construct, containing level1 plant expression cassettes for eSaCas9(pEPOR1CB0113), sgRNA{AT1G73460}(pEPOR1CB0099) and sgRNA{AT1G73460}(pEPOR1CB0110) to Protein kinase superfamily protein.
    Depositor
    Insert
    [35S:eSaCas9(pEPOR1CB0113) ] +[AtU6-26:sgRNA]
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 1, 2019
    Availability
    Academic Institutions and Nonprofits only
  8. pEPOR1CB0023

    Plasmid
    #117538
    Purpose
    Level1 Golden Gate Cassette: extended stem sgRNA cassette with AtU6-26 terminator for SpCas9, targeting NbPDS gene in N. benthamiana
    Depositor
    Insert
    sgRNA (Sp Cas9) - extended stem with U626 terminator
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Jan. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
  9. pEPOR2KN0080

    Plasmid
    #117647
    Purpose
    Level2 MoClo construct, containing level1 plant expression cassettes for eSaCas9(pEPOR1CB0113) and sgRNA_ENTH/ANT {AT1G14910}(pEPOR1CB0100)and sgRNA_ENTH/ANT {AT2G01600}(pEPOR1CB0111)
    Depositor
    Insert
    [35S:eSaCas9(pEPOR1CB0113) ] +[AtU6-26:sgRNA]
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Nov. 16, 2018
    Availability
    Academic Institutions and Nonprofits only
  10. pEPOR2KN0077

    Plasmid
    #117644
    Purpose
    Level2 MoClo construct, containing level1 plant expression cassettes for SaCas9(pEPOR1CB0015) and sgRNA_Protein kinase superfamily protein {AT1G73460}(pEPOR1CB0099)and sgRNA_Protein kinase superfamily protein {AT1G73460}(pEPOR1CB0110)
    Depositor
    Insert
    [35S:SaCas9(pEPOR1CB0015) ] +[AtU6-26:sgRNA]
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Nov. 16, 2018
    Availability
    Academic Institutions and Nonprofits only
  11. pEPOR2KN0076

    Plasmid
    #117643
    Purpose
    Level2 MoClo construct, containing level1 plant expression cassettes for eSaCas9 (pEPOR1CB0113), sgRNA {AT1G62600}(pEPOR1CB0098) and sgRNA PPR-Adenylate Cyclase {AT1G62590}(pEPOR1CB0109)
    Depositor
    Insert
    [35S:eSaCas9(pEPOR1CB0113) ] +[AtU6-26:sgRNA]
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Nov. 16, 2018
    Availability
    Academic Institutions and Nonprofits only
  12. pEPOR2KN0074

    Plasmid
    #117641
    Purpose
    Level2 MoClo construct, containing level1 plant expression cassettes for Sp-xCas9 3.7(pEPOR1CB0012) and sgRNA_AtIPCS1 {AT3G54020}(pEPOR1CB0093)and sgRNA_AtIPCS1 {AT3G54020}(pEPOR1CB0104)
    Depositor
    Insert
    [35S:Sp-xCas9 3.7(pEPOR1CB0012) ] +[AtU6-26:sgRNA]
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Nov. 16, 2018
    Availability
    Academic Institutions and Nonprofits only
  13. pEPOR2KN0073

    Plasmid
    #117640
    Purpose
    Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.1(pEPOR1CB0011) and sgRNA_AtIPCS1 {AT3G54020}(pEPOR1CB0093)and sgRNA_AtIPCS1 {AT3G54020}(pEPOR1CB0104)
    Depositor
    Insert
    [35S:Sp-eCas9 1.1(pEPOR1CB0011) ] +[AtU6-26:sgRNA]
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Nov. 16, 2018
    Availability
    Academic Institutions and Nonprofits only
  14. pEPOR2KN0072

    Plasmid
    #117639
    Purpose
    Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.0(pEPOR1CB0010) and sgRNA_AtIPCS1 {AT3G54020}(pEPOR1CB0093)and sgRNA_AtIPCS1 {AT3G54020}(pEPOR1CB0104)
    Depositor
    Insert
    [35S:Sp-eCas9 1.0(pEPOR1CB0010) ] +[AtU6-26:sgRNA]
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Nov. 16, 2018
    Availability
    Academic Institutions and Nonprofits only
  15. pEPOR2KN0071

    Plasmid
    #117638
    Purpose
    Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_AtIPCS1 {AT3G54020}(pEPOR1CB0093)and sgRNA_AtIPCS1 {AT3G54020}(pEPOR1CB0104)
    Depositor
    Insert
    [35S:SpCas9-h(pICSL11023) ] +[AtU6-26:sgRNA]
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Nov. 16, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 5751 - 5800 of 34443 results