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pSLIK S6K (E389-deltaCT) neo

Plasmid

#58516

Purpose

Lentiviral expression vector for an inducible HA-tagged rat S6K1-E389-deltaCT

Depositor

Kevin Janes

Article

Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

Insert

Rps6kb1 (Rps6kb1 Rat)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

T389E, stop codon at amino acid 402

Promoter

Available since

May 5, 2016

Availability

Academic Institutions and Nonprofits only
pLX317-RBFOX1

Plasmid

#115442

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

RBFOX1 (RBFOX1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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CMV-NFIB-T2A-miRFP670

Plasmid

#187222

Purpose

Expresses human NFIB and miRFP670 via T2A linker under control of a CMV promoter.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Insert

Nuclear Factor I B (NFIB Human)

Use

Tags

Inserted T2A-miRFP670 at 3' terminal of MCS.

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-BE3-FNLS(RA)

Plasmid

#112671

Purpose

CMV expression vector for BE3-FNLS construct (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3-FNLS

Use

Tags

3x FLAG

Expression

Mammalian

Mutation

NLS sequence at the N-terminus and D10A

Promoter

CMV

Available since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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P-Ef1a-mCherry-T2A-RPL22-1xV5

Plasmid

#170324

Purpose

Expresses V5 and mCherry tagged RPL22

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Tags

Expression

Mutation

None

Promoter

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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HA-BAP1-D672G-GFP

Plasmid

#78919

Purpose

Mammalian expression of BAP1 with D672G and an EGFP fusion

Depositor

Michael Hendzel

Article

Ismail et al Cancer Res. 2014 Aug 15;74(16):4282-94.

Insert

BAP1 (BAP1 Human)

Use

Tags

EGFP and HA

Expression

Mammalian

Mutation

D672G

Promoter

CMV

Available since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
pLKO-shSmad2

Plasmid

#37051

Depositor

Sam Thiagalingam

Article

Papageorgis et al Cancer Res. 2010 Feb 1;70(3):968-78. Epu

Insert

Smad2 (SMAD2 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 10, 2013

Availability

Academic Institutions and Nonprofits only
pLKO.1P POLE1_1

Plasmid

#160762

Purpose

Suppress POLE1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shPOLE1_1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pBabepuro3-p18Flag

Plasmid

#24933

Depositor

Lynda Chin

Article

Wiedemeyer et al Cancer Cell. 2008 Apr . 13(4):355-64.

Insert

P18 (CDKN2C Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2010

Availability

Academic Institutions and Nonprofits only
HA-BAP1-F81V-GFP

Plasmid

#78912

Purpose

Mammalian expression of BAP1 with F81V and an EGFP fusion

Depositor

Michael Hendzel

Article

Ismail et al Cancer Res. 2014 Aug 15;74(16):4282-94.

Insert

BAP1 (BAP1 Human)

Use

Tags

EGFP and HA

Expression

Mammalian

Mutation

F81V

Promoter

CMV

Available since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
pLXSN-Axl DN

Plasmid

#65223

Purpose

Expression of dominant negative Axl in mammalian cells

Depositor

Axel Ullrich

Article

Zhang et al Cancer Res. 2008 Mar 15;68(6):1905-15. d

Insert

AXL DN (AXL Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

deleted cytoplasmic domain

Promoter

LTR

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only
pEB3-miRFP703

Plasmid

#79994

Purpose

EB3 (microtubule-associated protein) labeling with near-infrared fluorescent protein miRFP703

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

EB3 (MAPRE3 Human)

Use

Tags

miRFP703

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pLKO.1 shJUND puro

Plasmid

#58698

Purpose

Lentiviral shRNA vector for knockdown of human JUND

Depositor

Kevin Janes

Article

Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

Insert

shJUND (JUND Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only
CD 44 pGL4basic

Plasmid

#78135

Purpose

luciferase reporter for miRNA activity

Depositor

Heidi Schwarzenbach

Article

Eichelser et al Oncotarget. 2014 Oct 30;5(20):9650-63.

Insert

CD 44 (CD44 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

CD44

Available since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

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FastLightR-bRaf-mVenus

Plasmid

#162155

Purpose

Expresses FastLightR-bRaf-mVenus in mammalian cells

Depositor

Andrei Karginov

Article

Shaaya et al Elife. 2020 Sep 23;9. pii: 60647. doi: 1

Insert

FastLightR-bRaf-mVenus

Use

Tags

mVenus

Expression

Mammalian

Mutation

Valine 600 is substituted with Glutamic acid

Promoter

CMV

Available since

Dec. 14, 2020

Availability

Academic Institutions and Nonprofits only

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HA-BAP1-A95D-GFP

Plasmid

#78914

Purpose

Mammalian expression of BAP1 with A95D and an EGFP fusion

Depositor

Michael Hendzel

Article

Ismail et al Cancer Res. 2014 Aug 15;74(16):4282-94.

Insert

BAP1 (BAP1 Human)

Use

Tags

EGFP and HA

Expression

Mammalian

Mutation

A95D

Promoter

CMV

Available since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
pICE-HA-CtIP-siR-WT

Plasmid

#82030

Purpose

Plasmid for constitutive or doxycycline-inducible expression of human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

Depositor

Patrick Calsou, Sébastien Britton

Article

Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

Insert

CtIP (RBBP8 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Mutations making the cDNA resistant to a siRNA

Promoter

CMV-tet

Available since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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HA-BAP1-H169Q-GFP

Plasmid

#78917

Purpose

Mammalian expression of BAP1 with H169Q and an EGFP fusion

Depositor

Michael Hendzel

Article

Ismail et al Cancer Res. 2014 Aug 15;74(16):4282-94.

Insert

BAP1 (BAP1 Human)

Use

Tags

EGFP and HA

Expression

Mammalian

Mutation

H169Q

Promoter

CMV

Available since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
pRetroX SgK269 WT

Plasmid

#68028

Purpose

expression of Sgk269 in mammalian cells

Depositor

Roger Daly

Article

Croucher et al Cancer Res. 2013 Mar 15;73(6):1969-80. d

Insert

PEAK1 (PEAK1 Human)

Use

Retroviral

Tags

FLAG/myc, HA, and IRES-DsRed

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only
pGL2 p15 (-756/+68) SBR2 Mut 3' box

Plasmid

#15711

Depositor

Joan Massague

Article

Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

Insert

p15 promoter (CDKN2B Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Mut 3' box in SBR2. The 3' box contains…

Promoter

Available since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only