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pJH2972

Plasmid

#175285

Purpose

Pmyo-3 GFP::DAF-16a unc-54 3' UTR C.elegans muscle expression of isoforms a of DAF-16

Depositor

Mei Zhen

Article

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

DAF-16a (daf-16 Nematode)

Tags

GFP

Expression

Worm

Promoter

Pmyo-3

Available Since

Nov. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pJW1820

Plasmid

#154321

Purpose

Multi-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

GFP^SEC (LoxP)^AID*::3xFLAG

Use

CRISPR and Cre/Lox

Expression

Worm

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pMLS269

Plasmid

#73727

Purpose

SapTrap SL2::SV40-NLS C-terminal connector donor plasmid

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

SL2 + SV40 NLS (gdp-2/gdp-3 intergenic region)

Use

CRISPR

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pWM_12x601_193 NRL + TetO

Plasmid

#157812

Purpose

EcoRV digestion produces 12x601 chromatin assembly construct with central TetO in addition to carrier DNA that prevents overassembly of nucleosomes

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

12x601_tetO

Expression

Bacterial

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-U6-sgRNA-CMV-puro

Plasmid

#169450

Purpose

backbone for sgRNA expression

Depositor

Stanley Qi

Article

Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

Insert

sgRNA

Use

Lentiviral

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pPD187.49

Plasmid

#1722

Depositor

Andrew Fire

Type

Empty backbone

Tags

T7p, SPGFPN, SPGFPC, Sp6p, OriF1>>, OriF1&l…

Expression

Worm

Available Since

March 7, 2005

Availability

Academic Institutions and Nonprofits only

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pJW1821

Plasmid

#163092

Purpose

CRISPR repair template: add insertion site specific homology arms by PCR before insertion

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

mScarlet^SEC (Lox511I)^::3xMyc

Use

CRISPR and Cre/Lox

Expression

Worm

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pJW1850

Plasmid

#154328

Purpose

sgRNA Target Vector for CRISPR

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

ttTi5605 site targeting sgRNA (F+E) with K09B11.2 U6 promoter and 3'UTR

Use

CRISPR

Expression

Worm

Mutation

F+E mutations in sgRNA

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pSA109

Plasmid

#59783

Purpose

drives ZIF-1 protein in C. elegans intestinal cells

Depositor

Jeremy Nance

Article

Armenti et al Development. 2014 Dec;141(23):4640-7. do

Inserts

elt-2 promoter (elt-2 Nematode)
zif-1 coding sequence (zif-1 Nematode)
unc-54 3' UTR

Available Since

Nov. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pDSP31

Plasmid

#199373

Purpose

Encodes dual cistronic nuclear localized GCaMP6f and mScarlet-I optimized for C. elegans expression

Depositor

Hang Lu

Insert

NLS::GCaMP6f::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS

Expression

Bacterial

Mutation

no mutations, except added subcellular localizati…

Available Since

April 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pTK73.ChrII_ttTi5605.sgRNA

Plasmid

#194058

Purpose

sgRNA targeting ChrII_ttTi5605 locus of the C. elegans genome

Depositor

Kota Mizumoto

Article

Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

Insert

ChrII_ttTi5605 sgRNA

Expression

Worm

Promoter

U6

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pTK73.ChrV_oxTi365.gRNA

Plasmid

#194052

Purpose

sgRNA targeting ChrV_oxTi365 locus of the C. elegans genome

Depositor

Kota Mizumoto

Article

Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

Insert

ChrV_oxTi365 sgRNA

Expression

Worm

Promoter

U6

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pJH2037

Plasmid

#179220

Purpose

Psra-11::UNC-7-UrSL-wCherry unc-54 3' UTR C.elegans neural expression of UNC-7-wCherry

Depositor

Mei Zhen

Article

Kawano et al Neuron. 2011 Nov 17;72(4):572-86. doi: 1

Insert

UNC-7 (unc-7 Nematode)

Tags

wCherry

Expression

Worm

Promoter

Psra-11

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCZGY2844

Plasmid

#87760

Purpose

Punc-17b-PH::miniSOG, expression of membrane targeted PH-miniSOG in cholinergic motor neurons.

Depositor

Andrew Chisholm

Article

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

Punc-17b-PH-miniSOG

Tags

PH

Expression

Worm

Promoter

Punc-17b

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pCFJ150-cul4

Plasmid

#80353

Purpose

Pmex-5:GFP::H2B::PEST::cul-4 3'UTR reporter construct used for microinjection

Depositor

Sean Ryder

Article

Kaymak et al Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.

Insert

Pmex-5:GFP::H2B::PEST::cul-4 3'UTR

Expression

Worm

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pJW4.02

Plasmid

#62867

Purpose

Bacterial expression of MBP-PGL-1

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

MBP-PGL-1

Tags

MBP

Expression

Bacterial

Available Since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNAOX-twk5

Plasmid

#16105

Depositor

Larry Salkoff

Article

Wei et al Neuropharmacology. 1996 . 35(7):805-29.

Insert

Tandem pore domain K+ channel (twk-5 Nematode)

Use

Mammalian expression & oocyte expression

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
pBSc + Shal

Plasmid

#16142

Depositor

Larry Salkoff

Article

Fawcett et al J Biol Chem. 2006 Oct 13. 281(41):30725-

Insert

Voltage-gated K+ channels (Y73B6BL.19 Nematode)

Use

Xenopus oocyte expression

Mutation

N/A

Available Since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
pJH2217

Plasmid

#179251

Purpose

Psra-11::TWK-18(gf)-UrSL-wCherry unc-54 3' UTR C.elegans neural expression of TWK-18-wCherry

Depositor

Mei Zhen

Article

Kawano et al Neuron. 2011 Nov 17;72(4):572-86. doi: 1

Insert

TWK-18 (twk-18 Nematode)

Tags

wCherry

Expression

Worm

Mutation

M280I

Promoter

Psra-11

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCFJ2214 - Intron GG1 - 900 bp

Plasmid

#159880

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron GG1 - 900 bp

Expression

Worm

Mutation

Not applicable

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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