We narrowed to 71,302 results for: nin

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pT3TS-spRYCas9-ABE-UMax

Plasmid

#222139

Purpose

Near PAMless Adenine base editor with modified TadA variant for A-to-G base editing in zebrafish

Depositor

Gaurav Varshney

Article

Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

Insert

TadA-nSpRYCas9

Use

CRISPR; Zebrafish expression

Tags

bipartite NLS

Promoter

T3

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pT3TS-spCas9-ABE-Umax-rest1

Plasmid

#222144

Purpose

Adenine base editor with modified TadA variant for restricted A-to-G base editing in zebrafish

Depositor

Gaurav Varshney

Article

Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

Insert

Modified TadA-nSpCas9

Use

CRISPR; Zebrafish expression

Tags

Bipartite NLS

Promoter

T3

Available Since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag4A-MITF-M (E87R)

Plasmid

#31152

Depositor

Yardena Samuels

Article

Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

Insert

MITF-M (MITF Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

Glutamic acid 87 changed to Arginine

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only
pLVX-EF1a-EGFP-VAMP2-IRES-Puromycin

Plasmid

#134661

Purpose

Expresses EGFP-tagged synaptic vesicle protein

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

vesicle-associated membrane protein 2 (VAMP2 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pSIN-TRE-SspB-mCh-p60

Plasmid

#190170

Purpose

Doxycycline-inducible lentivirus vector to express SspBmicro-mCh-p60, a recruitable katanin for microtubule severing, in mammalian cells.

Depositor

Anna Akhmanova

Article

Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

Insert

SspBmicro-mCherry-p60

Use

Lentiviral

Tags

SspBmicro-mCherry

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-MH6-RspWYL1

Plasmid

#108306

Purpose

Expresses E. coli codon optimized RspWYL1 in the pET28a backbone.

Depositor

Arbor Biotechnologies

Article

Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

Insert

RspWYL1

Use

CRISPR

Tags

mH6 tag

Expression

Bacterial

Promoter

lac

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pAT9676-ABE

Plasmid

#162997

Purpose

plasmid expressing ABE

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

ABE

Use

CRISPR

Tags

3xFLAG and SV40 NLS

Expression

Mammalian

Promoter

EF1-a

Available Since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-Myo9b R1695M

Plasmid

#134911

Purpose

Expression of rat Myo 9b (Myr 5) in mammalian cells.GAP minus mutant.

Depositor

Martin Bähler

Article

Muller et al Mol Biol Cell. 1997 Oct;8(10):2039-53. d

Insert

Myo 9b (rat) with 3' UTR. GAP minus mutant R1695M (Myo9b Rat)

Tags

EGFP

Expression

Mammalian

Mutation

changed arginine 1695 to methionine

Promoter

CMV IE

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag TSC2 (S939A/T1462A)

Plasmid

#14131

Depositor

Brendan Manning

Article

Manning et al Mol Cell. 2002 Jul . 10(1):151-62.

Insert

TSC2 S939A/T1462A (TSC2 Human)

Tags

Flag

Expression

Mammalian

Mutation

S939A/T1462A

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pGCP123-EbpA_g1

Plasmid

#153517

Purpose

sgRNA for ebpA gene (NT) under nisin-inducible nisA promoter, barcoded

Depositor

Kimberly Kline

Article

Afonina et al mBio. 2020 Oct 20;11(5):e01101-20. doi:

Insert

pnisA-sgRNA(EbpA_g1)-dCas9 scaffold

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pPBT-peRNA_GG-Puro

Plasmid

#173220

Purpose

piggyBac transposon containing hU6 promotor and pegRNA cloning site

Depositor

Jacob Giehm Mikkelsen

Article

Wolff et al Front Genome Ed. 2021 Nov 12;3:786893. d

Inserts

pegRNA cloning cassette
PuroR

Use

Piggybac

Expression

Mammalian

Promoter

EF1-a and hU6

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pL1-pKPI-MtLAP1-Adh

Plasmid

#193520

Purpose

Anthocyanin pigmentation based visual marker for arbuscular mycorrhizal fungal colonization of Medicago truncatula roots

Depositor

Jeremy Murray

Article

Kumar et al New Phytol. 2022 Sep 20. doi: 10.1111/np

Insert

MtLAP1

Expression

Plant

Promoter

Medtr8g059790(KPI)

Available Since

June 2, 2023

Availability

Academic Institutions and Nonprofits only

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pMALp2x-CARP-1

Plasmid

#41727

Depositor

Koji Okamoto

Article

Nakano et al Proc Natl Acad Sci U S A. 2006 Sep 12;10

Insert

clam ADP-ribosylating protein-1

Use

Bacterial subcloning

Mutation

deleted 5'-UTR nucleotides 1-64

Promoter

pMB1

Available Since

April 10, 2013

Availability

Academic Institutions and Nonprofits only
pRSF-G1mCNPRS

Plasmid

#174718

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of L-3-(2-cyano-4-pyridyl)alanine (mCNP), into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Abdelkader et al Angew Chem Int Ed Engl. 2022 Jan 31. doi

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available Since

Feb. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR MCS R4-R3

Plasmid

#62086

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a multiple cloning site. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

Mule gateway entry vector

Expression

Mammalian

Available Since

Feb. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-GFP ArgSen

Plasmid

#184704

Purpose

Lentiviral transfer plasmid for expression of GFP ArgSen

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

GFP ArgSen

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pATLIC

Plasmid

#110287

Purpose

A backbone vector for cell surface display in E. coli by Ligation Independent Cloning (LIC)

Depositor

In-Geol Choi

Article

Ko et al Appl Environ Microbiol. 2012 May;78(9):3

Insert

yfaL

Expression

Bacterial

Promoter

araBAD promoter

Available Since

April 22, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag TSC2 (S939A)

Plasmid

#14132

Depositor

Brendan Manning

Article

Manning et al Mol Cell. 2002 Jul . 10(1):151-62.

Insert

TSC2 S939A (TSC2 Human)

Tags

Flag

Expression

Mammalian

Mutation

S939A.

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3 Flag TSC2 (T1462A)

Plasmid

#14130

Depositor

Brendan Manning

Article

Manning et al Mol Cell. 2002 Jul . 10(1):151-62.

Insert

TSC2 T1462A (TSC2 Human)

Tags

Flag

Expression

Mammalian

Mutation

T1462A

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pKPI-MtLAP1

Plasmid

#193521

Purpose

Anthocyanin pigmentation based visual marker for arbuscular mycorrhizal fungal colonization of Medicago truncatula roots

Depositor

Jeremy Murray

Article

Kumar et al New Phytol. 2022 Sep 20. doi: 10.1111/np

Inserts

MtLAP1
DsRed

Expression

Plant

Promoter

ATUBI10 and Medtr8g059790(KPI)

Available Since

June 2, 2023

Availability

Academic Institutions and Nonprofits only

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