163,740 results

1. GG-EBNA-OSK2M2L1-PP

   Plasmid

   #102902

   Purpose

   EBNA episome plasmid for U6 promoter-driven expression of 10 gRNAs targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters. Includes PGK-puro selection cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   OSK2M2L1-gRNAs-PGK-puro
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR223_SNAI1_WT

   Plasmid

   #82164

   Purpose

   Gateway Donor vector containing SNAI1 , part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, William Hahn, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   SNAI1 (SNAI1 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDH-EF1-IRES eGFP

   Plasmid

   #128059

   Purpose

   Lentiviral expression of an insert from EF1 promoter and co-expression of EGFP. See Depositor Comments for additional information regarding plasmid sequence.

   Depositor

   Oskar Laur

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. Murine CD3 WTdelta-F2A-gamma-T2A-epsilon-P2A-zeta pMIG II

   Plasmid

   #52092

   Purpose

   Retroviral expression of all four WT CD3 subunits, co-expressed with EGFP

   Depositor

   Dario Vignali

   Article

   Holst et al Nat Immunol. 2008 Jun;9(6):658-66. doi:

   Inserts

   CD3 delta (Cd3d Mouse)
   CDC gamma (Cd3g Mouse)
   CD3 epsilon (Cd3e Mouse)
   CD3 zeta (Cd247 Mouse)
   

   Use

   Retroviral

   Tags

   F2A, IRES-EGFP, P2A, and T2A

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-C1-MKLP1

   Plasmid

   #70145

   Purpose

   mammalian expression of MKLP1 with an eGFP fusion

   Depositor

   Masanori Mishima

   Article

   Douglas et al Curr Biol. 2010 May 25;20(10):927-33. do

   Insert

   MKLP1 (KIF23 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. GST-TACC3-His6

   Plasmid

   #69106

   Purpose

   For bacterial expression of recombinant human TACC3 tagged with GST and His.

   Depositor

   Stephen Royle

   Article

   Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

   Insert

   Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
   

   Use

   Tags

   GST and His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-BRCA1

   Plasmid

   #119007

   Purpose

   Mammalian expression of human BRCA1 and BFP

   Depositor

   Ralf Kuehn

   Article

   Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

   Insert

   BRCA1 (BRCA1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCW-LIC-N8C_Fab3a

   Plasmid

   #203689

   Purpose

   Expresses N8C_Fab3a

   Depositor

   Brenda Schulman

   Article

   Henneberg et al Nat Chem Biol. 2023 Dec;19(12):1513-1523

   Insert

   N8C_Fab3a
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. Circular-oc-CVB3-GFP

   Plasmid

   #226262

   Purpose

   Production of in vitro circularizable RNA via IVT

   Depositor

   Prashant Mali

   Article

   Tong et al Nat Biomed Eng. 2024 Aug 26. doi: 10.103

   Insert

   EGFP
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-SARS-Spike

    Plasmid

    #145031

    Purpose

    Express SARS-CoV spike protein with C9 tag at C-terminal in mammalian cells

    Depositor

    Fang Li

    Article

    Shang et al Nature. 2020 Mar 30. pii: 10.1038/s41586

    Insert

    SARS-CoV Spike
    

    Use

    Tags

    C9 and CD5 signal peptide

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 16, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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