We narrowed to 34,848 results for: CaS;

1. p426_Cas9_gRNA-ARS308a

   Plasmid

   #87384

   Purpose

   All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS308a sequence CACTTGTCAAACAGAATATA in yeast chromosome 3

   Depositor

   Aindrila Mukhopadhyay

   Article

   Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

   Insert

   Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS308a
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   ADH1, pTyrosine

   Available Since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV_EE-NG-nCas9

   Plasmid

   #163542

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   EE-NG-nCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…

   Promoter

   Available Since

   Dec. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. U6_ ATM_G101_sgRNA_CAG_St1Cas9_CNRZ1066_v2

   Plasmid

   #214814

   Purpose

   A single vector containing a CAG-driven Cas9 variant from S. thermophilus recognizing a consensus NNACAA PAM (St1Cas9 CNRZ1066 v2) and its U6-driven sgRNA targeting human ATM

   Depositor

   Yannick Doyon

   Insert

   St1Cas9 CNRZ1066 v2 targeting ATM (ATM Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Cas9 NGFR

   Plasmid

   #155279

   Purpose

   Lentiviral Cas9 expression with NGFR marker

   Depositor

   Martin Carroll

   Insert

   spCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. RCASBP-Y NHA

   Plasmid

   #11479

   Depositor

   William Pavan

   Article

   Loftus et al DNA Res. 2001 Oct 31. 8(5):221-6.

   Insert

   Gateway ccdB reading frame cassette
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available Since

   March 22, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAtCas9-D10A_2

   Plasmid

   #91160

   Purpose

   Cas9 only expression, Cas9 Type: AtCas9_D10A (nickase), Plant Selection: 2x35S:npt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   AtCas9_D10A (nickase)
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   D10A

   Promoter

   Available Since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. SpCas9 TadCBEb

   Plasmid

   #193832

   Purpose

   Express TadCBEb (with SpCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadA-CDb-SpCas9 D10A-2xUGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCas9CR4-VQR

   Plasmid

   #120224

   Purpose

   pCas9CR4 with VQR mutations for changing PAM site specificity to NGAN or NGNG from Kleinstiver et al. 2015

   Depositor

   Christopher Reisch

   Insert

   SpCas9-VQR
   

   Use

   Synthetic Biology

   Tags

   ssrA

   Expression

   Mutation

   Mutations D1135V/R1335V/T1337R

   Promoter

   pTet

   Available Since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPbuCas13b-gRNA-NT

   Plasmid

   #184568

   Purpose

   Constitutive expression of single-spacer CRISPR array with non-targeting spacer for PbuCas13b in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

   Insert

   Constitutive expression of single-spacer CRISPR array witha nontargeting spacer for PbuCas13b in bacteria.
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available Since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCasper4-QF#7-hsp70

    Plasmid

    #46135

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Insert

    QF#7
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Aug. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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