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Showing: 4641 - 4680 of 8321 results

  1. RHPMP126 (mOrange2-alpha Tubulin)

    Plasmid
    #88835
    Purpose
    Localization by Super-Resolution microscopy of alpha-Tubulin fused to mOrange2
    Depositor
    Ricardo Henriques
    Insert
    alpha-tubulin (TUBA1B) (TUBA1B Human)
    Use
    Tags
    mOrange2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    88835-plasmid-map-sequence-id-177488

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  2. MAP3K5-TAP

    Plasmid
    #69726
    Purpose
    Express tandem tagged MAP3K5 in cells
    Depositor
    Daniel Liebler
    Insert
    Apoptosis signal-regulating kinase 1 (MAP3K5 Human)
    Use
    Tags
    HA-FLAG tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    69726-plasmid-map-sequence-id-123455

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  3. 4QQD

    Plasmid
    #61947
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    UHRF1:SDC130-H02:C39674 (UHRF1 Human)
    Use
    Tags
    6xHis and MHHHHHHSSGRENLYFQG
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    March 2, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  4. YWHAB

    Plasmid
    #39128
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    YWHAB (YWHAB Human)
    Use
    Tags
    Tev His6
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  5. CLYBL hOPM

    Plasmid
    #112499
    Purpose
    Homologous recombination vector for dox-inducible overexpression of OTX2, PAX6, and MITF in CLYBL locus
    Depositor
    Bruce Conklin
    Insert
    OTX2, PAX6, MITF (PAX6, MITF, OTX2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    112499-plasmid-map-sequence-id-223597

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  6. pCMV-T7-ABEmax-SpRY

    Plasmid
    #195279
    Purpose
    Allows for in vitro transcription of original pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025) without EGFP
    Depositor
    Carsten Lederer, Petros Patsali
    Insert
    human codon optimized ABEmax(7.10) SpCas9 variant named SpRY without EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    195279-plasmid-map-sequence-id-394869

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  7. CACNA2D1-TwinStrepII

    Plasmid
    #83192
    Purpose
    Expression of tagged protein in mammalian cells
    Depositor
    Peter Barr-Gillespie
    Insert
    CACNA2D1 (Cacna2d1 Rat)
    Use
    Tags
    Twin Strep Tag II
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    83192-plasmid-map-sequence-id-163487

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  8. pCLIPf-H2B Control Plasmid

    Plasmid
    #101134
    Purpose
    Expression of CLIP-tagged Histone H2B in mammalian cells
    Depositor
    Ana Egana, New England Biolabs
    Insert
    Histone H2B (H2BC11 Human)
    Use
    Tags
    CLIP-tag (CLIPf)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 27, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101134-plasmid-map-sequence-id-194159

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  9. hGli1 6x his

    Plasmid
    #84923
    Purpose
    mammalian expression of Gli1
    Depositor
    Martin Fernandez-Zapico
    Insert
    Gli1 (GLI1 Human)
    Use
    Tags
    6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 14, 2016
    Availability
    Academic Institutions and Nonprofits only

  10. pGEX 6P1 Cyclin A (555)

    Plasmid
    #61844
    Purpose
    bacterial expression of GST cyclin A
    Depositor
    Jonathon Pines
    Insert
    cyclin A (CCNA2 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    L2V
    Promoter
    tac
    Available since
    June 8, 2015
    Availability
    Academic Institutions and Nonprofits only

  11. pTF277 (pFA6a-TEV-6xGly-2xStrep-KanMX)

    Plasmid
    #44094
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    2xStrep tag, 6xGly linker, ADH1 transcription ter…
    Expression
    Mutation
    Promoter
    Available since
    April 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    44094-plasmid-map-sequence-id-61681

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  12. pTRIPZ (M)-HT-Cbx7

    Plasmid
    #82515
    Purpose
    Lentivirus vector. Expresses Halotag-Cbx7 fusion proteins in mammalian cells.
    Depositor
    Xiaojun Ren
    Insert
    Chromobox Homolog 7 (CBX7 Human)
    Use
    Lentiviral
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    cmv
    Available since
    Dec. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    82515-plasmid-map-sequence-id-156790

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  13. pAAV asyn S129D

    Plasmid
    #36069
    Depositor
    Patrick Aebischer, Hilal Lashuel
    Insert
    alpha-synuclein S129D (SNCA Human)
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    S129D
    Promoter
    CMV
    Available since
    Sept. 10, 2012
    Availability
    Academic Institutions and Nonprofits only

  14. pScarlessHD-DsRed-w+

    Plasmid
    #80801
    Purpose
    Vector for generating dsDNA donors for HDR. Contains the visible marker 3xP3-DsRed flanked by piggyBac termini and GMR-white for identifying backbone incorporation.
    Depositor
    Kate O'Connor-Giles
    Inserts
    piggyBac-3xP3-DsRed
    GMR-white
    Use
    High copy, amp resistance
    Tags
    Expression
    Mutation
    Promoter
    3xP3 and GMR::hsp70
    Available since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    80801-plasmid-map-sequence-id-152801

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  15. pTF279 (pFA6a-TEV-6xGly-2xStrep-HphMX)

    Plasmid
    #44082
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    2xStrep tag, 6xGly linker, ADH1 transcription ter…
    Expression
    Mutation
    Promoter
    Available since
    April 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    44082-plasmid-map-sequence-id-61684

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  17. YWHAG

    Plasmid
    #36903
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    YWHAG (YWHAG Human)
    Use
    Tags
    6xHis with TEV Cleavage
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 21, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  19. pUltra-H2B-mTagBFP2

    Plasmid
    #161791
    Purpose
    3rd generation lentiviral multicistronic vector for mammalian expression of EGFP, H2B-mTagBFP2, and one gene of interest
    Depositor
    Jorge Luis Arias-Arias
    Insert
    H2B-mTagBFP2
    Use
    Lentiviral; Multicistronic
    Tags
    EGFP and mTagBFP2
    Expression
    Mammalian
    Mutation
    I174A in the mTagBFP2 protein according to PLoS O…
    Promoter
    UbC
    Available since
    Nov. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    161791-plasmid-map-sequence-id-316818

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  20. pAAV-CaMKIIa-eArchT3.0-P2A-EGFP-WPRE-hGHpA

    Plasmid
    #51110
    Purpose
    Forebrain principal neuron expression of eArchT3,0 with physically uncoupled EGFP fluorophore
    Depositor
    Jonathan Ting
    Insert
    eArchT3.0
    Use
    AAV
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CaMKIIa
    Available since
    April 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    51110-plasmid-map-sequence-id-79323

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  21. ZFYVE16

    Plasmid
    #39099
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ZFYVE16 (ZFYVE16 Human)
    Use
    Tags
    His6-Zb-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  22. Flag-hCEP55

    Plasmid
    #190429
    Purpose
    Expresses human CEP55 in mammalian cells
    Depositor
    Ken-Ichi Takemaru
    Insert
    CEP55 (CEP55 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    190429-plasmid-map-sequence-id-371157

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  23. pQCXIP-mCherry-TAZ

    Plasmid
    #128351
    Purpose
    Retrovirus vector to express mCherry-TAZ
    Depositor
    Yutaka Hata
    Insert
    TAZ (WWTR1 Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    128351-plasmid-map-sequence-id-254110

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  24. hC73S-pEGFP-N1

    Plasmid
    #111536
    Purpose
    Expresses human KCNB1 C73S in mammalian cells for imaging
    Depositor
    Federico Sesti
    Insert
    human C73S-Kv2.1 (KCNB1 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    C73S
    Promoter
    CMV
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    111536-plasmid-map-sequence-id-211633

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  25. BDD FVIII H4

    Plasmid
    #46774
    Purpose
    pcDNA4.BDD FVIII.H4(R484H)
    Depositor
    Robert Peters
    Insert
    B-domain deleted FVIII H4 (R484H) (F8 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    R484H
    Promoter
    CMV
    Available since
    Nov. 26, 2013
    Availability
    Academic Institutions and Nonprofits only
    46774-plasmid-map-sequence-id-66260

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  26. CBIG-RORb

    Plasmid
    #48709
    Purpose
    bicistronic expression of RORb and EGFP
    Depositor
    Jeffrey Macklis
    Insert
    RORb (Rorb Mouse)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV/beta-actin
    Available since
    Oct. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    48709-plasmid-map-sequence-id-70664

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  27. H11-CAG-loxP-FNF-bpA-3pA-loxP-tdTomato

    Plasmid
    #180156
    Purpose
    tdTomato cre reporter with Hipp11 recombineering homology arms
    Depositor
    Mario Capecchi
    Insert
    tdTomato
    Use
    Cre/Lox and Mouse Targeting
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    180156-plasmid-map-sequence-id-360161

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  28. T1-araC-Pbad-D10A/H840A cas9 ABCD-pACYC-BB

    Plasmid
    #110547
    Purpose
    CRISPR interference; works in conjunction with an sgRNA expression vector to silence genes in the E. coli chromosome.
    Depositor
    Ichiro Matsumura
    Insert
    dCas9
    Use
    Tags
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Promoter
    pBAD
    Available since
    July 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    110547-plasmid-map-sequence-id-210556

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  29. pE-SUMO CRYBB2

    Plasmid
    #80749
    Purpose
    Expression of human beta B2-crystallin in E. coli
    Depositor
    Larry David
    Insert
    CRYBB2 (CRYBB2 Human)
    Use
    Tags
    6xHIS and Smt3 SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    80749-plasmid-map-sequence-id-153512

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  30. KSP_K560GFP

    Plasmid
    #129770
    Purpose
    Expresses Xenopus Kinesin-5 KSP head and neck linker, dimerized through kinesin-1 neck-coil and coil-1 to 560 and GFP tagged
    Depositor
    William Hancock
    Insert
    KSP (kif11.L Frog)
    Use
    Tags
    GFP and His6
    Expression
    Bacterial
    Mutation
    Truncation at 367, fused to kinesin-1 starting Al…
    Promoter
    Available since
    Nov. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    129770-plasmid-map-sequence-id-263613

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  31. pTRIPZ (M)-HT-Cbx6

    Plasmid
    #82514
    Purpose
    Lentivirus vector. Expresses Halotag-Cbx6 fusion proteins in mammalian cells.
    Depositor
    Xiaojun Ren
    Insert
    Chromobox Homolog 6 (CBX6 Human)
    Use
    Lentiviral
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    cmv
    Available since
    Dec. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    82514-plasmid-map-sequence-id-156788

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  32. XBB.1.5+L455F+F456L

    Plasmid
    #208588
    Purpose
    Mammalian expression vector for expressing XBB.1.5+L455F+F456L SARS-CoV-2 Spike, with 19AA cytoplasmic tail truncation
    Depositor
    Ben Murrell
    Insert
    SARS-CoV-2 variant XBB.1.5+L455F+F456L spike (S )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    208588-plasmid-map-sequence-id-409581

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  33. BDD FVIII H3

    Plasmid
    #46775
    Purpose
    pcDNA4.BDD FVIII.H3(M2238V)
    Depositor
    Robert Peters
    Insert
    B-domain deleted FVIII H3 protein (M2238V) (F8 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    M2238V
    Promoter
    CMV
    Available since
    Aug. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    46775-plasmid-map-sequence-id-66268

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  34. pCDZ2FE_Rev3_1873-2025_4PA

    Plasmid
    #105673
    Purpose
    Expresses Flag-tagged fragment (amino acids 1873-2025) of human Rev3 protein with mutations abolishing Rev7 binding
    Depositor
    Thanos Halazonetis
    Insert
    Rev3L (REV3L Human)
    Use
    Tags
    2X Flag tag
    Expression
    Mammalian
    Mutation
    Mutations: P1880A, P1885A, P1996A, P2001A
    Promoter
    Available since
    March 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    105673-plasmid-map-sequence-id-203204

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  35. pcDNA3 H2B-mIFP T2A Mpro (C145A)

    Plasmid
    #163080
    Purpose
    Expresses inactive Mpro mutant and a near-infrared fluorescent protein mIFP fused to H2B
    Depositor
    Xiaokun Shu
    Insert
    Inactive Mpro mutant C145A and H2B-mIFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    163080-plasmid-map-sequence-id-320827

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  36. pCHA_OROV_NSs

    Plasmid
    #208939
    Purpose
    Expresses C-terminally HA-tagged Oropouche orthobunyavirus NSs protein from CMV promoter in mammalian cells
    Depositor
    Gerald Barry
    Insert
    NSs
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Deleted 66 bp from 5' end and deleted 415 b…
    Promoter
    CMV
    Available since
    Jan. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    208939-plasmid-map-sequence-id-419030

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  37. TBC1D7

    Plasmid
    #32047
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    TBC1D7 (TBC1D7 Human)
    Use
    Tags
    His (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 3, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  38. hsKIF5B(1-560)-HaloTag-FRB

    Plasmid
    #213899
    Purpose
    Imaging of KIF5B fused to HaloTag
    Depositor
    Lukas Kapitein
    Insert
    hsKIF5B(aa1-560)-HaloTag-FRB (KIF5B Human)
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    213899-plasmid-map-sequence-id-426209

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  39. isoform PKC Zeta 1 (JCE875)

    Plasmid
    #182027
    Purpose
    yeast expression of mouse PKC Zeta isoform 1 with yeast PKC1 promoter and ADH1 terminator
    Depositor
    Juan Carlos Igual
    Insert
    PKC Zeta isoform 1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    yeast PKC1 promoter (-600 to -1)
    Available since
    May 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    182027-plasmid-map-sequence-id-362001

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  40. Foxd3-pIRES2 eGFP

    Plasmid
    #37269
    Depositor
    Patricia Labosky
    Insert
    Foxd3 (Foxd3 Mouse)
    Use
    Tags
    IRES-GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 16, 2012
    Availability
    Academic Institutions and Nonprofits only
Showing: 4641 - 4680 of 8321 results