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pME Lamin B Vhh mNeonGreen-pA (JDW 1312)

Plasmid

#224501

Purpose

Gateway compatible middle entry clone containing Lamin B nanobody fused to mNeonGreen with HA Tag (For visualizing the nuclear lamina, lamin chromobody)

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Lamin B Vhh mNeonGreen-pA

Use

Gateway Cloning

Available since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pCA12-pHR-SFFV-BFP-B23

Plasmid

#199443

Purpose

Expression of BFP-B23 in mammalian cells

Depositor

Baohui Chen

Publication

Fu et al J Cell Biol. 2023 Jan 2;222(1):e20220211

Insert

B23 (NPM1 Human)

Use

Lentiviral

Tags

TagBFP

Expression

Mammalian

Promoter

SFFV

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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14x PP7 with loxP-kanMX-loxP

Plasmid

#189939

Purpose

Integration of 14xPP7 loops in Saccharomyces cerevisiae. Selection by KanMX (flanked by LoxP sites for marker removal)

Depositor

Tineke Lenstra

Publication

Donovan et al EMBO J. 2019 Jun 17;38(12). pii: embj.20

Insert

14x PP7 with loxP-kanMX-loxP

Tags

kanMX

Expression

Bacterial

Available since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHR pUAS-dGFP pPGK-iRFP

Plasmid

#176530

Purpose

Lentiviral vector expressing a UAS-driven destabilized GFP, as well as a PGK promoter-driven iRFP marker.

Depositor

Jared Toettcher

Publication

Ravindran et al Cell Syst. 2022 Feb 16;13(2):131-142.e13

Inserts

dGFP
iRFP

Use

Lentiviral

Expression

Mammalian

Promoter

PGKp and UASp

Available since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-FLAG-APEX2-LGALS8

Plasmid

#175758

Purpose

Lentiviral vector expressing N-terminally tagged APEX2-FLAG-LGALS8

Depositor

Wade Harper

Publication

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

LGALS8 (LGALS8 Human)

Use

Lentiviral

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-hSyn-mRuby3-6xFLAG

Plasmid

#161613

Purpose

Cell morphology marker construct. Contains a red fluorescent protein mRuby3 and six FLAG tags.

Depositor

Edward Boyden

Publication

Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

Insert

mRuby3-6xFLAG

Use

AAV

Expression

Mammalian

Mutation

N/A

Promoter

Human synapsin 1 promoter

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-SYN_mMaroon-KIX-mMaroon

Plasmid

#137009

Purpose

AAV backbone, mMaroon-KIX acceptor for R-CREB

Depositor

Ryohei Yasuda

Publication

Laviv et al Neuron. 2019 Dec 17. pii: S0896-6273(19)

Insert

KIX domain (Crebbp Rat)

Use

AAV

Tags

mMaroon

Expression

Mammalian

Promoter

hSynapsin

Available since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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BAS288 C terminal mKO2 natR

Plasmid

#74083

Purpose

S.pombe recoded mKO2 in universal C-terminal tagging plasmid, nourseothricin resistance.

Depositor

Bassem Al-Sady

Publication

Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

Insert

mKO2

Available since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only
MXS_tdTomato

Plasmid

#62407

Purpose

MXS_chaining vector with tdTomato

Depositor

Pierre Neveu

Publication

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

tdTomato

Use

Synthetic Biology

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR223-MPP7

Plasmid

#23411

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MPP7 (MPP7 Human)

Use

Gateway Cloning

Mutation

2MM

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA5-FRT/T0-Flag-RECQL5

Plasmid

#222620

Purpose

Mammalian expression vector of flag-tagged RECQL5

Depositor

Daniel Durocher

Publication

Zhao et al Mol Cell. 2023 Aug 3;83(15):2792-2809.e9

Insert

RECQL5 (RECQL5 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

July 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pUC18T-mini-Tn7T-Gm-Pc-mNeonGreen

Plasmid

#205022

Purpose

mini-Tn7 vector for insertion of mNeonGreen (codon optimized for P. fluorescens) at bacterial attTn7 site

Depositor

Rosemarie Wilton

Publication

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

mNeonGreen

Expression

Bacterial

Mutation

mNeonGreen is codon optimized for expression in P…

Promoter

Pc

Available since

Sept. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-JEDI-1P-Kv-WPRE

Plasmid

#202608

Purpose

Double floxed soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector expressed under the mammalian promoter (EF1a)

Depositor

Francois St-Pierre

Publication

Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

Insert

JEDI-1P-Kv

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1a

Available since

June 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-JEDI-1P-WPRE

Plasmid

#202611

Purpose

Double floxed genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector expressed under the mammalian promoter (EF1a)

Depositor

Francois St-Pierre

Publication

Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

Insert

JEDI-1P

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1a

Available since

June 21, 2023

Availability

Academic Institutions and Nonprofits only

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N-WASP

Plasmid

#186132

Purpose

For expression of recombinant N-WASp in bacteria cells.

Depositor

Michael Rosen

Publication

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

WASL (WASL Rat)

Tags

His6

Expression

Bacterial

Mutation

Amino Acid residues 183-193 fused to 273-501

Promoter

T7

Available since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Gene Trap - 24XMS2 Lentiviral Vector

Plasmid

#174198

Purpose

Lentiviral expression vector coding for Blasticidin, iRFP and 24X MS2 hairpins. All inserts are cloned in on the -strand to allow for splicing in when targeted into an intron of transcribed genes.

Depositor

Daniel Larson

Publication

Wan et al Cell. 2021 May 27;184(11):2878-2895.e20.

Insert

iRFP

Use

Lentiviral

Tags

24X MS2 hairpins, Blasticidin, and T2A

Available since

Sept. 28, 2021

Availability

Academic Institutions and Nonprofits only

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R26-pCAG-Ceru-10unit-BGHpA

Plasmid

#158387

Purpose

10-unit intMEMOIR recording array for mammalian cells

Depositor

Michael Elowitz

Publication

Chow et al Science. 2021 Apr 9;372(6538). pii: 372/

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

intMEMOIR 10-unit array

Expression

Mammalian

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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MXS_PGK::PuroR-bGHpA

Plasmid

#62440

Purpose

MXS_chaining vector with PGK::PuroR-bGHpA

Depositor

Pierre Neveu

Publication

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

resistance cassette against Puromycin with PGK Promoter

Use

Synthetic Biology

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_PGK::ZeoR-bGHpA

Plasmid

#62442

Purpose

MXS_chaining vector with PGK::ZeoR-bGHpA

Depositor

Pierre Neveu

Publication

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

resistance cassette against Zeocin with PGK Promoter

Use

Synthetic Biology

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pSox2-bd::FP

Plasmid

#34703

Depositor

Hollis Cline

Publication

Bestman et al J Comp Neurol. 2012 Feb 1;520(2):401-33.

Insert

eGFP

Use

Xenopus expression

Tags

6x Sox2/Oct3-4 transcription factor binding domai…

Available since

Feb. 17, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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