163,637 results

1. pMS31: pTwinStrep-SUMO-PmcTnsAB

   Plasmid

   #168164

   Purpose

   PmcTnsAB expression construct for protein purification.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Insert

   PmcCAST TnsAB
   

   Use

   Tags

   6X His, Twin Strep and SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBiFC-VN155(I152L)

   Plasmid

   #27097

   Depositor

   Chang-Deng Hu

   Article

   Kodama et al Biotechniques. 2010 Nov . 49(5):793-805.

   Insert

   VN155(I152L)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   I152L

   Promoter

   Available Since

   Jan. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTK229

   Plasmid

   #71256

   Purpose

   luciferase reposter with 229 bp of the HSV TK promoter

   Depositor

   Peter Cockerill

   Article

   Hawwari et al J Immunol. 2002 Aug 15;169(4):1876-86.

   Insert

   Thymidine Kinase promoter
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   HSV Thymidine Kinase

   Available Since

   Nov. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1(+)- e18

   Plasmid

   #131670

   Purpose

   Expresses an engineered variant of RAD18 ("e18") that stimulates CRISPR mediated HDR.

   Depositor

   Alberto Ciccia

   Article

   Nambiar et al Nat Commun. 2019 Jul 30;10(1):3395. doi:

   Insert

   e18 (engineered RAD18 with SAP domain deleted) (RAD18 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. SPLICSFAST-ER-MT-P2A

   Plasmid

   #214892

   Purpose

   Expresses equimolar Outer mitochondrial membrane- and Endoplasmic Reticulum- targeted split-FAST fragments for Short-range ER-Mito Contact sites

   Depositor

   Tito Calì

   Insert

   SPLICSFAST-ER-MT-P2A
   

   Use

   Tags

   none

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3-Flag-IDH1

   Plasmid

   #62906

   Purpose

   Expresses wildtype IDH1 in mammalian cells

   Depositor

   Yue Xiong

   Article

   Zhao et al Science. 2009 Apr 10;324(5924):261-5. do

   Insert

   isocitrate dehydrogenase 1 (NADP+), soluble (IDH1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJIT163-eAFID-3

   Plasmid

   #167360

   Purpose

   For inducing predictable deletions in protoplasts and wheat plants

   Depositor

   Caixia Gao

   Article

   Wang et al Nat Biotechnol. 2020 Dec;38(12):1460-146

   Insert

   A3Bctd-Cas9-UDG-T2A-AP lyase
   

   Use

   Tags

   Expression

   Plant

   Mutation

   WT

   Promoter

   Available Since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. TFORF2164

   Plasmid

   #142186

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   GRHL2 (GRHL2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AAV-hSyn-EGFP

   Plasmid

   #114213

   Purpose

   AAV vector (control) that targets expression of EGFP to neurons

   Depositor

   Xue Han

   Article

   Keaveney et al Cell Rep. 2018 Jul 10;24(2):294-303. doi

   Insert

   Enhanced Green Fluorescent Protein
   

   Use

   AAV and Mouse Targeting

   Tags

   None

   Expression

   Mammalian

   Mutation

   None

   Promoter

   human synapsin (hSyn)

   Available Since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLX304-GW-DCK*-IRES-GFP

    Plasmid

    #176291

    Purpose

    Gateway vector for use in generating POI-DCK* fusion reporter

    Depositor

    William Kaelin

    Article

    Koduri et al Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6

    Insert

    Mutant Deoxycytidine Kinase (DCK*, S74E R104M D133A) (DCK Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    S74E R104M D133A

    Promoter

    CMV

    Available Since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge