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Plasmid#160545PurposepYES2 derivative reporter plasmid encoding PREV1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.DepositorInsertUseTagsExpressionYeastMutationN/APromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only
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pCFEX1-A2aGH
Plasmid#160546PurposepYC2/CT derivative reporter plasmid encoding Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.DepositorInsertUseTagsExpressionYeastMutationN/APromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCFEX2-A2aGH
Plasmid#160547PurposepYC2/CT derivative reporter plasmid encoding PPGI1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.DepositorInsertUseTagsExpressionYeastMutationN/APromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCFEX3-A2aGH
Plasmid#160548PurposepYC2/CT derivative reporter plasmid encoding PREV1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.DepositorInsertUseTagsExpressionYeastMutationN/APromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
Donor_N_FH_Piwi
Plasmid#186651PurposeDonor plasmid to endogenously tag piwi gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.DepositorInsertOSS PIWI N-terminal 3xFlag-3xHA Tag Donor Plasmid (piwi Fly)
UseTags3xFlag-3xHAExpressionInsectMutationPromoterAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
Cas9_ANKRD1_sgRNA2
Plasmid#186669PurposePlasmid containing Cas9 and ANKRD1 sgRNA2 , sgRNA sequence: cggtcagcttatatagct.DepositorInsertANKRD1 KO sgRNA Plasmid (ANKRD1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET303C-hSOD1 A4V/C6S
Plasmid#139667PurposeExpression plasmids containing human A4V/C6S SOD1DepositorInsertSuperoxide dismutase-1 (SOD1 Human)
UseTagsExpressionBacterialMutationchange alanine 4 to valine, change cysteine 6 to …PromoterT7Available sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-HsGIGYF2_35-105-E46AE47FN91A-GB1_AF
Plasmid#148686PurposeBacterial Expression of HsGIGYF2_35-105-E46AE47FN91ADepositorInsertHsGIGYF2_35-105-E46AE47FN91A (GIGYF2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pYPQ288 (BoCas12a)
Plasmid#138125PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized BoCas12a without promoterDepositorInsertBoCas12a
UseCRISPR; Gateway compatible cas12a entry cloneTagsNLSExpressionPlantMutationPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ285 (Lb5Cas12a)
Plasmid#138120PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized Lb5Cas12a without promoterDepositorInsertLb5Cas12a
UseCRISPR; Gateway compatible cas12a entry cloneTagsNLSExpressionPlantMutationPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ286 (CMaCas12a)
Plasmid#138122PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized CMaCas12a without promoterDepositorInsertCMaCas12a
UseCRISPR; Gateway compatible cas12a entry cloneTagsNLSExpressionPlantMutationPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ233-STU
Plasmid#138111PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized, catalytically deactivated LbCas12a-SRDX repressor without promoter and terminatorDepositorInsertdLbCas12a-SRDX
UseCRISPR; Gateway compatible cas12a entry cloneTagsNLSExpressionPlantMutationPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ239-STU
Plasmid#138112PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized FnCas12a without promoter and terminatorDepositorInsertFnCas12a
UseCRISPR; Gateway compatible cas12a entry cloneTagsNLSExpressionPlantMutationPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Flag-PolĂŸ(TM-KTM)-IRES-neo
Plasmid#128675Purposemammalian expression of Flag-PolĂŸ(TM-KTM: L301R/V303R/V306R-K41R/K61R/K81R) protein with neomycin selectionDepositorInsertPolB (POLB Human)
UseLentiviralTagsFlagExpressionMammalianMutationL301R/V303R/V306R-K41R/K61R/K81RPromoterCMVAvailable sinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Flag-PolĂŸ(TM/K206A/K244A)-IRES-puro
Plasmid#128684Purposemammalian expression of Flag-PolĂŸ(TM/K206A/K244A: L301R/V303R/V306R/K206A/K244A)) protein with puromycin selectionDepositorInsertPolB (POLB Human)
UseLentiviralTagsFlag tagExpressionMammalianMutationL301R/V303R/V303R/K206A/K244APromoterCMVAvailable sinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-EBA175-Flag(E1418K/A1419R/S1421R/P1424Q/Y1426S)
Plasmid#155010PurposeExpresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 E1418K/A1419R/S1421R/P1424Q/Y1426S variant (residues 1284-1462) from pcDNA3.1DepositorInsertPfEBA175
UseTagsFlag, GFP, and signal peptide (residues 1 to 32) …ExpressionMammalianMutationE1418K/A1419R/S1421/P1424Q/Y1426S; insert codes f…PromoterCMVAvailable sinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Neo-NCOA7g3 (BB36)
Plasmid#139454PurposeLentiviral vector with gRNA targeting human NCOA7 short isoform; includes neomycin selectable markerDepositorInsertNCOA7 short isoform-targeting sgRNA inserted; resistance gene: neoR (NCOA7 Synthetic)
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1a / U6Available sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Neo-NCOA7g2 (BB35)
Plasmid#139453PurposeLentiviral vector with gRNA targeting human NCOA7 short isoform; includes neomycin selectable markerDepositorInsertNCOA7 short isoform-targeting sgRNA inserted; resistance gene: neoR (NCOA7 Synthetic)
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1a / U6Available sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-His6-K2P4.1A-mCherry-StreptagII
Plasmid#158743PurposeExpresses K2P4.1 isoform A with TEV protease cleavable N- and C-terminal affinity tags.DepositorInsertK2P4.1-A (KCNK4 Human)
UseTagsHis6 and mCherry with Streptag IIExpressionYeastMutationResidues 1-264 with N-linked glycosylation sites …PromoterAOX1Available sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTetDBl-CLIP-CPAP
Plasmid#136865PurposeMammalian expression of the centrosomal protein CENPJ N-terminally fused to CLIP-tagDepositorInsertCLIP-CENPJ (CENPJ Human, Synthetic)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTetDBl-CLIP-Cep290
Plasmid#136854PurposeMammalian expression of the centrosomal protein Cep290 N-terminally fused to CLIP-tagDepositorInsertCLIP-Cep290 (CEP290 Human, Synthetic)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTetBl-CLIP-Cep290
Plasmid#136853PurposeMammalian expression of the centrosomal protein Cep290 N-terminally fused to CLIP-tagDepositorInsertCLIP-Cep290 (CEP290 Human, Synthetic)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTetD-SNAP-CPAP
Plasmid#136816PurposeMammalian expression of the centrosomal protein CENPJ N-terminally fused to SNAP-tagDepositorInsertSNAP-CENPJ (CENPJ Human, Synthetic)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJET1.2_Bod1l_Anti-Sense
Plasmid#124444PurposePlasmid for anti-sense in situ probe in vitro transcriptionDepositorInsertBiorientation of chromosomes in cell division 1-like (Bod1l Mouse)
UseIn situ probeTagsExpressionMutationPromoterT7Available sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLentiCMV-Blast mLST8-Flag Mut1-2
Plasmid#124918PurposeFor stable expression sgRNA resistant Flag tagging mLST8 Q44E/N46L and W272F/W274F mutantDepositorInsertMLST8 (MLST8 Human)
UseLentiviralTagsFlagExpressionMammalianMutationInsert ORF was sirently mutated to be resistent t…PromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJET1.2_Gli3_Sense
Plasmid#124439PurposePlasmid for sense in situ probe in vitro transcriptionDepositorInsertGLI-Kruppel family member GLI3 (Gli3 Mouse)
UseIn situ probeTagsExpressionMutationPromoterT7Available sinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
ORAI1-YFP V107M-L273D-L276D
Plasmid#114183PurposeORAI1 channel with C-terminal YFP, carrying the constitutively activating mutation V107M from TAM, and the mutations L273D-L276D leading to a deficiency in STIM1-mediated gating.DepositorInsertORAI1-YFP V107M-L273D-L276D (ORAI1 Human)
UseTagsYFPExpressionMammalianMutationV107M/L273D/L276DPromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
ORAI1-YFP T184M-L273D-L276D
Plasmid#114184PurposeORAI1 channel with C-terminal YFP, carrying the activating mutation T184M from TAM, and the mutations L273D-L276D leading to a deficiency in STIM1-mediated gating.DepositorInsertORAI1-YFP T184M-L273D-L276D (ORAI1 Human)
UseTagsYFPExpressionMammalianMutationT184M/L273D/L276DPromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCOG40-HRS (257-276) Hs
Plasmid#83236PurposepGST-HRS (257-276) Hs, Expresses the GST-HRS in E.coliDepositorInsertHRS (257-276) Hs (HGS Human)
UseTagsExpressionBacterialMutation258-277PromoterAvailable sinceNov. 22, 2016AvailabilityAcademic Institutions and Nonprofits only