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We narrowed to 5,937 results for: plasmid dna

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Showing: 5851 - 5900 of 5937 results

  1. AcrVA1_cpGR2(GGS5)-S103ΔELS_(GGS)2-(GGS)2

    Plasmid
    #246051
    Purpose
    Expression of AcrVA1-cpGR2 hybrid in mammalian cells; insertion before S103 with deletion of surrounding amino acids ELS and GGS2 linkers; enables chemogenetic control of MbCas12a
    Depositor
    Dominik Niopek
    Article
    Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk
    Insert
    AcrVA1_cpGR2-S103ΔELS
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Mutation
    ΔELS
    Available Since
    Dec. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    246051-plasmid-map-sequence-id-495343

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  3. Rescue myc-PCDHGC5 mRNA

    Plasmid
    #122229
    Purpose
    Rescue mRNA for Protocadherin gamma C5 knock down with sh1 shRNA (plasmid 122227). With five silent mutations at sh1 shRNA target site.
    Depositor
    Angel de Blas
    Article
    Li et al J Neurosci. 2012 Aug 22;32(34):11780-97.
    Insert
    PCDHGC5 (Pcdhgc5 Rat)
    Tags
    9E10 cMyc epitope (EQKLISEEDL) was inserted betwe…
    Expression
    Mammalian
    Mutation
    Silent mutations are in five consecutive codons …
    Available Since
    March 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    122229-plasmid-map-sequence-id-237759

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  9. pCR2.1_HBB_A

    Plasmid
    #87848
    Purpose
    pCR2.1-based plasmid holding a 328-bp fragment containing the exon-1-exon-2 splice border of aberrant HBB[IVSI-110(G>A)] human β-globin mRNA
    Depositor
    Marina Kleanthous
    Article
    Patsali et al Int J Mol Sci. 2020 Sep 11;21(18). pii:
    Insert
    Homo sapiens hemoglobin subunit beta (HBB), Aberrant cDNA fragment (Exon1/19nt mispliced Intron1 + Exon2) (HBB Human)
    Expression
    Bacterial
    Mutation
    HBB(IVSI-110) β-thalassaemia misplicing mutation …
    Available Since
    Oct. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    87848-plasmid-map-sequence-id-313713

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  10. MAP3K6-TAP

    Plasmid
    #69727
    Purpose
    Express tandem tagged MAP3K6 in cells
    Depositor
    Daniel Liebler
    Insert
    Apoptosis signal-regulating kinase 2 (MAP3K6 Human)
    Tags
    HA-V5 tag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    69727-plasmid-map-sequence-id-128182

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  11. MAP3K5-TAP

    Plasmid
    #69726
    Purpose
    Express tandem tagged MAP3K5 in cells
    Depositor
    Daniel Liebler
    Insert
    Apoptosis signal-regulating kinase 1 (MAP3K5 Human)
    Tags
    HA-FLAG tag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    69726-plasmid-map-sequence-id-123455

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  12. MAP3K15-TAP

    Plasmid
    #69728
    Purpose
    Express tandem tagged MAP3K15 in cells
    Depositor
    Daniel Liebler
    Insert
    Apoptosis signal-regulating kinase 3 (MAP3K15 Human)
    Tags
    HA-Myc tag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    69728-plasmid-map-sequence-id-128183

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  15. pARC8-LambdaRedBeta-EcSSB-EcMutLE32K

    Plasmid
    #162576
    Purpose
    Arabinose inducible recombineering plasmid encoding Lambda-Red Beta with EcSSB for use with dsDNA templates and enhanced recombination efficiency. Contains a dominant negative mismatch repair protein.
    Depositor
    George Church
    Article
    Filsinger et al Nat Chem Biol. 2021 Jan 18. pii: 10.1038
    Inserts
    Lambda Red-Beta
    E. coli SSB
    EcMutLE32K
    Expression
    Bacterial
    Mutation
    E32K mutated to give dominant negative phenotype
    Promoter
    pBAD
    Available Since
    May 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    162576-plasmid-map-sequence-id-326400

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  28. SpyTag-Alternate Quartet

    Plasmid
    #214728
    Purpose
    In mammalian cells expresses a Quartet of receptor-binding domains (RBDs) from the sarbecoviruses pang17, RmYN02, Rf1, and WIV1 each separated by GS-linkers with an N-terminal SpyTag fusion
    Depositor
    Mark Howarth
    Article
    Hills et al Nat Nanotechnol. 2024 May 6. doi: 10.103
    Insert
    SpyTag-Alternate Quartet
    Tags
    SpyTag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    214728-plasmid-map-sequence-id-435533

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  29. SpyTag-Quartet [SARS1]

    Plasmid
    #214729
    Purpose
    In mammalian cells expresses a Quartet of receptor-binding domains (RBDs) from the sarbecoviruses SHC014, Rs4081, RaTG13, and SARS-CoV each separated by GS-linkers with an N-terminal SpyTag fusion
    Depositor
    Mark Howarth
    Article
    Hills et al Nat Nanotechnol. 2024 May 6. doi: 10.103
    Insert
    SpyTag-Quartet [SARS1] (S Synthetic)
    Tags
    SpyTag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    214729-plasmid-map-sequence-id-435534

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  30. pLenti-nSpCas9-APOBEC1

    Plasmid
    #209039
    Purpose
    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where APOBEC1 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    APOBEC1-nSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG-HA
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    Tight TRE promoter
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209039-plasmid-map-sequence-id-413619

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  31. pLenti-nSpCas9-evoCDA1

    Plasmid
    #209042
    Purpose
    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where evoCDA1 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    evoCDA1-nSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG-HA
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    Tight TRE promoter
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209042-plasmid-map-sequence-id-413615

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  32. pLenti-nSpCas9-APOBEC3A

    Plasmid
    #209040
    Purpose
    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where APOBEC3A is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    APOBEC3A-nSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG-HA
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    Tight TRE promoter
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209040-plasmid-map-sequence-id-413592

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  33. pLenti-nSpCas9-BEACON2

    Plasmid
    #209041
    Purpose
    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where BEACON2 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    BEACON2-nSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG-HA
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    Tight TRE promoter
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209041-plasmid-map-sequence-id-419488

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  38. GluN2B/NR2B glutamate receptor scFv [N59/20]

    Plasmid
    #182092
    Purpose
    Mammalian Expression of GluN2B/NR2B glutamate receptor scFv. Derived from hybridoma N59/20.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    recombinant mouse scFv targeting GluN2B/NR2B glutamate receptor (Rattus norvegicus) (Grin2b Mouse)
    Tags
    Sortase, His
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    182092-plasmid-map-sequence-id-357627

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  48. anti-NPY/Neuropeptide Y scFv [L115/13] with sortase tag

    Plasmid
    #204426
    Purpose
    Mammalian Expression of anti-Neuropeptide Y scFv with a sortase tag for direct dye conjugation. Derived from hybridoma L115/13.
    Depositor
    Jeff Lichtman
    Article
    Han et al Nat Commun. 2024 Aug 5;15(1):6648. doi:
    Insert
    Recombinant mouse scFv targeting NPY/Neuropeptide Y (Homo sapiens)
    Tags
    6xHis tag and Sortase tag
    Expression
    Mammalian
    Available Since
    Sept. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204426-plasmid-map-sequence-id-406621

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  49. lucMAPT-30D

    Plasmid
    #214672
    Purpose
    MAPT Exon 10 reporter containing the MS2 hairpin 30 base pairs downstream of the 5′ splice site
    Depositor
    Eugene Yeo
    Article
    Schmok et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038
    Insert
    MAPT (MAPT Human)
    Tags
    Firefly Luciferase, Renilla Luciferase, and V5
    Expression
    Mammalian
    Mutation
    Contains Exon 9, Exon 10 with a mutation to intro…
    Promoter
    CMV
    Available Since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    214672-plasmid-map-sequence-id-424908

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  50. lucMAPT-30U

    Plasmid
    #214673
    Purpose
    MAPT Exon 10 reporter containing the MS2 hairpin 30 base pairs upstream of the 3′ splice site
    Depositor
    Eugene Yeo
    Article
    Schmok et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038
    Insert
    MAPT (MAPT Human)
    Tags
    Firefly Luciferase, Renilla Luciferase, and V5
    Expression
    Mammalian
    Mutation
    Contains Exon 9, Exon 10 with a mutation to intro…
    Promoter
    CMV
    Available Since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    214673-plasmid-map-sequence-id-424933

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Showing: 5851 - 5900 of 5937 results