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pC-KIT6

Plasmid

#118988

Purpose

Mutational dissection of the G rich region of the c-kit promoter

Depositor

Bjorn Hogberg

Article

Ducani et al J Am Chem Soc. 2019 Jul 3;141(26):10205-

Insert

c-kit proto-oncogene {promoter} (KIT Human)

Expression

Mammalian

Mutation

mutK2-SP-mutK1

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 FYN

Plasmid

#67106

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

FYN (FYN Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pISH-Dlx3

Plasmid

#74342

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Dlx3. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

distal-less homeobox 3 (Dlx3 Mouse)

Available Since

April 8, 2016

Availability

Academic Institutions and Nonprofits only

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c-myc promoter (4x TBE1 - mut)

Plasmid

#16598

Depositor

Bert Vogelstein

Article

He et al Science. 1998 Sep 4. 281(5382):1509-12.

Insert

c-myc promoter (4xTBE1 mut) (MYC Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

mutant TBE1 sites

Available Since

March 24, 2008

Availability

Academic Institutions and Nonprofits only
pCLC3sFlag

Plasmid

#52422

Purpose

mammalian expression of flag tagged rat CLC3 short transcript

Depositor

Steven Weinman

Article

Shimada et al Am J Physiol Gastrointest Liver Physiol.

Insert

CLC3 (Clcn3 Rat)

Tags

flag

Expression

Mammalian

Promoter

CMV

Available Since

May 7, 2014

Availability

Academic Institutions and Nonprofits only

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kank2_pJet1.2

Plasmid

#121980

Purpose

for generating kank2 riboprobes for WISH in zebrafish

Depositor

GuangJun Zhang

Article

Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

Insert

kank2 (LOC571549 Zebrafish)

Use

Cloning vector

Promoter

T7

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pTX208

Plasmid

#89268

Purpose

Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsYSA gene, OsYSA-gRNA01 and OsYSA-gRNA02

Depositor

Daniel Voytas

Article

Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

Insert

OsYSA-gRNA01 and OsYSA-gRNA02

Use

CRISPR

Tags

SV40 NLS

Expression

Plant

Promoter

Maize Ubiquitin1 promoter

Available Since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-Hook3 aa 1-239

Plasmid

#74609

Purpose

Expresses Hook domain and first coiled coil of Hook3 in bacteria for purification

Depositor

Ron Vale

Article

Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

Insert

human Hook3 amino acids 1-239 (HOOK3 Human)

Tags

6x His, Strep II, and superfolder GFP

Expression

Bacterial

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pGSTag Flag Jnk3a1(2nd methione)

Plasmid

#15746

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk3a1-2nd M (MAPK10 Human)

Expression

Bacterial

Available Since

March 6, 2008

Availability

Academic Institutions and Nonprofits only
pRK-flag-USP19 494-1290

Plasmid

#78580

Purpose

Expression flag-USP19 494-1290 in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-USP19 494-1290 (USP19 Human)

Tags

flag

Expression

Mammalian

Mutation

deleted amino acid 1-493 and mutated 1291 residue…

Promoter

CMV

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only
pGEX4T-Mst1 SARAH

Plasmid

#84297

Purpose

Expresses Mst1 SARAH domain for interaction studies.

Depositor

Jonathan Chernoff

Article

Rawat et al Curr Biol. 2016 Jun 20;26(12):1556-63. d

Insert

Mst1 (STK4 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only
pISH-Meis2

Plasmid

#74349

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Meis2. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Meis homeobox 2 (Meis2 Mouse)

Available Since

May 9, 2016

Availability

Academic Institutions and Nonprofits only

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TU#1806_CRISPR_unc-73_exon21

Plasmid

#82360

Purpose

to create unc-73E null allele

Depositor

Martin Chalfie

Article

Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Cas9 and sgRNA against unc-73 exon21 (unc-73 Nematode)

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pInducer21 Flag-Sesn1b

Plasmid

#61865

Purpose

Dox inducible lentivirial expression of Flag-Sesn1b (mouse)

Depositor

Ming Li

Article

Peng et al Cell. 2014 Sep 25;159(1):122-33. doi: 10

Insert

Sesn1 (Sesn1 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

none

Available Since

Jan. 16, 2015

Availability

Academic Institutions and Nonprofits only
pGSTag Flag Jnk3a2(2nd methione)

Plasmid

#15747

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk3a2(2nd methione) (MAPK10 Human)

Expression

Bacterial

Available Since

March 6, 2008

Availability

Academic Institutions and Nonprofits only
pET28a-Hook3 aa 1-160

Plasmid

#74607

Purpose

Expresses Hook domain of Hook3 in bacteria for purification

Depositor

Ron Vale

Article

Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

Insert

human Hook3 amino acids 1-160 (HOOK3 Human)

Tags

6x His, Strep II, and superfolder GFP

Expression

Bacterial

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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HOXD13 (chicken) HIS-tag pET

Plasmid

#8555

Depositor

Corey Largman

Article

Shen et al J Biol Chem 1997 Mar 28;272(13):8198-206

Insert

HOXD13 (HOXD13 )

Tags

His and T7

Expression

Bacterial

Mutation

contains 10 nt 5' to ATG start; makes prote…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pISH-Meis1

Plasmid

#74348

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Meis1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Meis homeobox 1 (Meis1 Mouse)

Available Since

April 12, 2016

Availability

Academic Institutions and Nonprofits only

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myc-actin-NES-1

Plasmid

#60618

Purpose

Encodes MYC tagged beta-actin with a mutation in the NES

Depositor

Primal de Lanerolle

Article

Hofmann et al J Cell Biol. 2009 Jul 27;186(2):193-200.

Insert

Beta-actin (ACTB Human)

Tags

MYC

Expression

Bacterial and Mammalian

Mutation

I178A, L180A

Available Since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only
M71::Myc6-IRES-taulLacZ LNL TV

Plasmid

#15619

Depositor

Peter Mombaerts

Article

Feinstein et al Cell. 2004 Jun 11. 117(6):833-46.

Insert

M71::Myc6-IRES-taulLacZ LNL TV (Myc Mouse)

Use

High copy number

Available Since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only