Addgene

We narrowed to 114,439 results for: CIL

Showing: 2341 - 2360 of 114439 results

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pQFT-mNGsacB

Plasmid

#203676

Purpose

pQFT with mNeonGreen and sacB under control of PQJ

Depositor

Urs Jenal

Article

Klotz et al Appl Environ Microbiol. 2023 May 18:e002

Inserts

mNeonGreen
sacB

Use

Tags

Expression

Bacterial

Mutation

Has synonymous (silent) mutations to remove BsrGI…

Promoter

PQJ

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPR2_IHFdist_rm

Plasmid

#162318

Purpose

P. aeruginosa PA14 CRISPR2 locus, with the IHFdistal binding site removed

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Use

Tags

Expression

Bacterial

Mutation

IHF distal site is removed

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-6-Integrase13-9

Plasmid

#197728

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

Integrase13 (tomato optimized)

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pMpGWB302-er-mCherry

Plasmid

#186287

Purpose

Binary vector for the expression of er-mCherry (SP-mCherry-HDEL) in plants (for Agrobacterium-mediated genetic transformation and visualization of endoplasmic reticulum)

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

Insert

er-mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pR

Plasmid

#193746

Purpose

Expresses LuxR under the control of BBa_J23102 constitutive promoter, ColE1 origin of replication, Ampicillin selection

Depositor

Sangram Bagh

Article

Sarkar et al ACS Synth Biol. 2021 Oct 15;10(10):2456-

Insert

LuxR

Use

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23102

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pTA2EGFP

Plasmid

#193756

Purpose

Expresses EGFP under the control of PLTetO-1 promoter, ColE1 origin of replication, Ampicillin selection

Depositor

Sangram Bagh

Article

Sarkar et al Chem Sci. 2021 Nov 9;12(48):15821-15832.

Insert

EGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

PLTetO-1

Available since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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pJA2LUXR(F)

Plasmid

#193755

Purpose

Expresses LuxR in forward direction under the control of BBa_J23102 constitutive promoter, ColE1 origin of replication, Ampicillin selection

Depositor

Sangram Bagh

Article

Sarkar et al Chem Sci. 2021 Nov 9;12(48):15821-15832.

Insert

LuxR

Use

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23102

Available since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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pMpGE_En03-sgRNA-Target1 (MpRTN1)

Plasmid

#186291

Purpose

Gateway entry vector for sgRNA (target 1: MpRTN1). Transient expression of sgRNA (Target 1: MpRTN1) for MpRTN1 in plant cells

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

Insert

sgRNA-Target1

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pcloDF-amp

Plasmid

#184844

Purpose

Backbone that confers Ampicillin resistance, contains cloDF ori

Depositor

Chase Beisel

Article

Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pMpGE010-sgRNA-Target1 (MpRTN1)

Plasmid

#186295

Purpose

Binary vector for CRISPR/Cas9 (target 1: MpRTN1) in plants (for Agrobacterium-mediated genetic transformation)

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

Insert

sgRNA-Target1

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pET28b-bhaA

Plasmid

#167810

Purpose

Expresses His6-tagged BhaA in E. coli

Depositor

Wilfred van der Donk

Article

Daniels et al Nat Chem. 2022 Jan;14(1):71-77. doi: 10.

Insert

BhaA

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pET28b-bhaB1

Plasmid

#167811

Purpose

Expresses His6-tagged BhaB1 in E. coli

Depositor

Wilfred van der Donk

Article

Daniels et al Nat Chem. 2022 Jan;14(1):71-77. doi: 10.

Insert

BhaB1

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pET28b-bhaB7

Plasmid

#167813

Purpose

Expresses His6-tagged BhaB7 in E. coli

Depositor

Wilfred van der Donk

Article

Daniels et al Nat Chem. 2022 Jan;14(1):71-77. doi: 10.

Insert

BhaB7

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pFE-dabA2

Plasmid

#133001

Purpose

pFE carrying a dabA2 gene (Uniprot: D0KWS7)

Depositor

David Savage

Article

Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

Insert

dabA2

Use

Tags

Expression

Bacterial

Mutation

Promoter

tetR

Available since

Nov. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pFE-dabB2

Plasmid

#133000

Purpose

pFE carrying a dabB2 gene (Uniprot: D0KWS8)

Depositor

David Savage

Article

Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

Insert

dabA2

Use

Tags

Expression

Bacterial

Mutation

Promoter

tetR

Available since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pCCG 306

Plasmid

#131167

Purpose

PCR template for genomic C-terminal Cub-R-URA3 tag, URA3 complements the uracil auxotrophy of S. cerevisiae ura mutant

Depositor

Nils Johnsson

Article

Dunkler et al Methods Mol Biol. 2015;1346:151-68. doi:

Type

Empty backbone

Use

Tags

CCG

Expression

Yeast

Mutation

Promoter

Available since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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S11_EcoRV_CAMKIIA_AscI

Plasmid

#128475

Purpose

CAMKII1 promoter

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

CAMKII1 promoter

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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S10_EcoRV_hcSYN_AscI

Plasmid

#128474

Purpose

hcSYN promoter

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

hcSYN promoter

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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p005-RFP-weak

Plasmid

#108316

Purpose

Synthetic RFP based on Corynactis spp under weak synthetic constitutive promoter pFAB4024; kanamycin resistant.

Depositor

Jaroslaw Bryk

Article

Siddall et al bioRxiv 2021.06.20.449138

Insert

RFP under controlf of weak synthetic constitutive promoter pFAB4024

Use

Tags

Expression

Bacterial

Mutation

Promoter

pFAB4024

Available since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pET21a_Msm_RbpA

Plasmid

#87928

Purpose

Mycobacterium smegmatis RpbA in pET21a (ampR) used to co-express with Msm SigA for crystallization purposes

Depositor

Seth Darst

Article

Hubin et al Elife. 2017 Jan 9;6. pii: e22520. doi: 1

Insert

RbpA

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7P

Available since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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