We narrowed to 2,462 results for: LARGE

1. pTol2-DEST-Hsp70-zCreI-mTagBFP2d-2xins (JDW 1002)

   Plasmid

   #229817

   Purpose

   A gateway compatible Tol2 destination vector containing the Hsp70 promoter driving Cre and TagBFP followed by 2 cHS4 insulator cores.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Type

   Empty backbone

   Use

   Tol2 destination vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDestTol2pA-2xIns-fli1ep-zCreI-BFP (JDW 1218)

   Plasmid

   #229819

   Purpose

   A gateway compatible Tol2 destination vector containing the fli1a promoter driving Cre and TagBFP in endothelial cells followed by 2 cHS4 insulator cores.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Type

   Empty backbone

   Use

   Tol2 destination vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAGEN-Luciferase-P2A-H2A-mCherry (JDW 1129)

   Plasmid

   #229823

   Purpose

   A CAGGS driven luciferase reporter followed by a P2A cleavage peptide and an H2A mCherry cassette for nuclear labeling.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Luciferase-P2A-H2A-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTol2-DEST-hsp70-zCreI-BFP (JDW 1184)

   Plasmid

   #229831

   Purpose

   A gateway compatible Tol2 destination vector containing the hsp70 promoter driving Cre and TagBFP in endothelial cells followed by 2 cHS4 insulator cores.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Type

   Empty backbone

   Use

   Tol2 destination vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pB-Tet-On-Luciferase-P2A-H2A-mCherry (JDW 945)

   Plasmid

   #229837

   Purpose

   A Piggybac compatible, tet-on, NEO selectable, vector for expressing luciferase followed by a P2A cleavage peptide and H2A fused mCherry.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Luciferase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTol2-Ubi-LexA-oMDC-pA-LexOp-nlsGFP-pA (JDW 1381)

   Plasmid

   #229838

   Purpose

   A Tol2 based expression vector for RU486 inducible expression of GFP in the nucleus with a destablized lex transactivator.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   LexA-mODC-LexAOp
   

   Use

   Tol2 based expression vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pB-Tet-On-Luciferase-P2A-H2A-mCherry (JDW 1154)

   Plasmid

   #229840

   Purpose

   A Piggybac compatible, tet-on, dual reporter encoding luciferase followed by a P2A cleavage peptide and then an H2A fused mCherry for nuclear labeling.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Luciferase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pME_Golgi-BFP_P2A_H2A_tdiRFP (JDW 1079)

   Plasmid

   #224491

   Purpose

   Gateway compatible middle entry clone containing GalT-mTagBFP-HA-P2A-H2A-tdiRFP (BFP golgi reporter and tandem infrared RFP nuclear reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Golgi-mTagBFP-P2A-H2A-tdiRFP
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pME-rox-nls-mCherry-V5-2xStop-rox (JDW 1167)

   Plasmid

   #224524

   Purpose

   A Gateway compatible middle entry clone containing a Rox flanked 3xNLS-mCherry-V5-2xStop cassette

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   rox-3xNLS-mCherry-V5-2xSTOP-rox
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p5E-EFS (EF1a core) (JDW 1319)

    Plasmid

    #224474

    Purpose

    Gateway compatible 5' entry clone with human alpha 2 globin pause site (to prevent transcription readthrough) and EF1a core promoter. Lacks downstream intron for a lower-activity, compact promoter.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    EFS promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pME-Luciferase-P2A-H2A-mCherry (JDW 926)

    Plasmid

    #224488

    Purpose

    Gateway compatible middle entry clone containing a Luciferase P2A H2A-mCherry dual reporter (Luciferase/nuclear red fluorescent reporter)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Luciferase-P2A-H2A-mCherry
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pME-LexA-mODC-LexOP-35SCaMV (JDW 1348)

    Plasmid

    #224505

    Purpose

    Gateway compatible middle entry clone containing a Destablized LexA and LexOperon with minimal CaMV 35S promoter as an all in one casette for RU486 driven gene expression

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    LexA-mODC-LexOP-35SCaMV
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pME-LexA-mODC-LexOP-c-Fos (JDW 1359)

    Plasmid

    #224506

    Purpose

    Gateway compatible middle entry clone containing a Destablized LexA and LexOperon with 3' minimal c-Fos promoter for an all in one RU486 driven gene expression construct

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    LexA-oMDC-LexOP-c-Fos
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pME TAEL2.0-5xC120-minpro (JDW 1109)

    Plasmid

    #224507

    Purpose

    Gateway compatible middle entry clone containing TAEL2.0 transcription factor and 5xC120 response element (All in one binary TAEL and effector domain clone)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    TAEL2.0-5xC120
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pME-3xHA-BirA-T2A-mCherry-caax (JDW 889)

    Plasmid

    #224512

    Purpose

    A Gateway compatible middle entry clone containing Biotin Ligase followed by T2A and mCherry membrane reporter (biotinylation and flourescent cell membrane reporter)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    3xHA-BirA-T2A-mCherry-Caax
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pME-lox-3xNLS-mCherry-V5-SV40pA-3xstop-lox (JDW 1232)

    Plasmid

    #224522

    Purpose

    A Gateway compatible middle entry clone containing loxP flanked 3xNLS-mCherry-V5-3xSTOP cassette

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    lox-3xNLS-mCherry-V5-SV40pA-3xstop-lox
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKTop::sptse5-CT (1169-1229)

    Plasmid

    #192948

    Purpose

    For membrane insertion study; expresses Tse5-CT (1169-1229)/PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus

    Depositor

    David Albesa-Jové

    Article

    Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

    Insert

    sptse5-CT (1169-1229)
    

    Use

    Tags

    PelB signal peptide

    Expression

    Bacterial

    Mutation

    encodes for tse5 (1169-1229)

    Promoter

    Available since

    Dec. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pKTop::tse5-CT (1169-1300)

    Plasmid

    #192956

    Purpose

    For membrane insertion study; Expresses tse5-CT (1169-1300) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus

    Depositor

    David Albesa-Jové

    Article

    Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

    Insert

    tse5-CT (1169-1300)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    encodes for tse5 (1169-1300)

    Promoter

    Available since

    Dec. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKTop::tse5-CT (1169-1317)

    Plasmid

    #192957

    Purpose

    For membrane insertion study; Expresses tse5-CT (1169-1317) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus

    Depositor

    David Albesa-Jové

    Article

    Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

    Insert

    tse5-CT (1169-1317)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    encodes for tse5 (1169-1317)

    Promoter

    Available since

    Dec. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKTop::tse5-CT (1169-1269)

    Plasmid

    #192954

    Purpose

    For membrane insertion study; Expresses tse5-CT (1169-1269) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus

    Depositor

    David Albesa-Jové

    Article

    Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

    Insert

    tse5-CT (1169-1269)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    encodes for tse5 (1169-1269)

    Promoter

    Available since

    Dec. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge