163,653 results

1. pAGM47523

   Plasmid

   #153221

   Purpose

   Level 0 zCas9i vector

   Depositor

   Sylvestre Marillonnet

   Article

   Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

   Insert

   SpCas9 with introns and 2 NLS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. ACBE-Q

   Plasmid

   #204608

   Purpose

   Mammalian expression of high-accuracy adenine-to-cytosine base editor ACBE-Q

   Depositor

   Dali Li

   Article

   Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103

   Insert

   bpNLS-N terminal of nSpCas9-linker-mAAG (R165E/Y179F)-TadA-8e (N108Q)-linker-C terminal of nSpCas9-bpNLS-P2A-GFP
   

   Use

   CRISPR

   Tags

   6*His

   Expression

   Mammalian

   Mutation

   TadA-8e (N108Q); mAAG (R165E/Y179F); S. pyogenes …

   Promoter

   Available Since

   Aug. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSPL

   Plasmid

   #110743

   Purpose

   Plasmid for the insect cell expression system, donor for plasmid fusions, two promoters, Multiplication Module M, LoxP site

   Depositor

   Tim Richmond

   Article

   Fitzgerald et al Nat Methods. 2006 Dec;3(12):1021-32. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin and p10 promoters

   Available Since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. SPLICS Mt-ER- Po Long P2A

   Plasmid

   #164117

   Purpose

   Detect the long Peroxisomes-mitochondria-Endoplasmic reticulum contact

   Depositor

   Marisa Brini, Tito Calì

   Article

   Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

   Insert

   Split GFP and YFP Mt-ER- Po Long P2A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMJA181

   Plasmid

   #128539

   Purpose

   Expresses anti-Prunus dulcis scFv Ab+BirA tag

   Depositor

   Marcos Alcocer

   Article

   de la Cruz et al J Biotechnol. 2016 Jun 10;227:112-9. doi

   Insert

   scFv Ab clone PD1F6 plus BirA tag
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   AOX1

   Available Since

   July 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. mCherry-Vimentin-N-18

   Plasmid

   #55158

   Purpose

   Localization: Intermediate Filaments, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   Vimentin (VIM Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-UbC

   Plasmid

   #62806

   Purpose

   To express genes at high levels in neuronal cells. This UbC promoter is more active in neurons than the promoter in CMV-based vectors.

   Depositor

   Jae Lee, Pantelis Tsoulfas

   Article

   Soderblom et al Eneuro. 2015 Mar-Apr;2(2). doi: 10.1523/

   Type

   Empty backbone

   Use

   AAV; Eucaryotic

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hUbC

   Available Since

   March 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmCherry-PH(Akt2)

   Plasmid

   #86596

   Purpose

   Expresses mCherry-tagged PH(WT) domain of human Akt2

   Depositor

   Thomas Leonard, Ivan Yudushkin

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt2 PH domain (AKT2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Amino acids 1 - 108

   Promoter

   Available Since

   April 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDESTmycIGF2BP3

   Plasmid

   #19879

   Depositor

   Thomas Tuschl

   Article

   Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

   Insert

   Insulin-like growth factor 2 mRNA binding protein 3 (IGF2BP3 Human)
   

   Use

   Tags

   MYC

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. AARS_C1_pNIC-Bio3

    Plasmid

    #153046

    Purpose

    Expression construct for producing Avi tagged Alanyl-tRNA synthetase

    Depositor

    Susanne Gräslund

    Insert

    AARS (AARS Human)
    

    Use

    Tags

    Avi and His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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